Biology and Fertility of Soils

, Volume 44, Issue 5, pp 797–803 | Cite as

Impact of irrigation water quality on soil nitrifying and total bacterial communities

  • Ndèye Yacine Badiane Ndour
  • Ezékiel Baudoin
  • Aliou Guissé
  • Mountakha Seck
  • Mamadou Khouma
  • Alain Brauman
Short Communication

Abstract

Disturbance induced by two contrasting irrigation regimes (groundwater versus urban wastewater) was evaluated on a sandy agricultural soil through chemical and microbial analyses. Contrary to wastewater, groundwater displayed very high nitrate contents but small amounts of ammonium and organic matter. Despite these strong compositional shifts, soil organic carbon and nitrogen, nitrate and ammonium contents were not significantly different in both types of irrigated plot. Moreover, neither microbial biomass nor its activity, determined as fluorescein diacetate hydrolysis activity, was influenced by irrigation regimes. Bacterial community structure, assessed by denaturing gradient gel electrophoresis (DGGE) of 16S ribosomal DNA fragments, was also weakly impacted as molecular fingerprints shared an overall similarity of 85%. Ammonia-oxidizing bacterial community (AOB) was monitored by DGGE of the functional molecular marker amoA gene (alpha subunit of the ammonia monooxygenase). Surprisingly, no amoA signals were obtained from plots irrigated with groundwater, whereas signal intensities were high in all plots under wastewater. Among the last, compositional shifts of the AOB community were weak. Overall, impact of irrigation water quality on soil chemistry could not be evidenced, whereas effects were low on the total bacterial compartment but marked on the AOB community.

Keywords

Irrigation Wastewater Nitrogen Ammonia-oxydizing bacteria (AOB) Tropical sandy soil 

Notes

Acknowledgements

This work was funded by the IRD-Département Soutien Formation. Chemical analyses were performed by iso 9001 LAMA Laboratory (Dakar, US Imago, IRD). The authors thank L. Dieng for useful technical advice about the DGGE fingerprinting method, P. Diagne at the head of PROVANIA (association of peri-urban farmers in Dakar), as well as all farmers implied in this study.

References

  1. Adam G, Duncan H (2001) Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soil. Soil Biol Biochem 33:943–951CrossRefGoogle Scholar
  2. Amato M, Ladd JN (1988) Assay for microbial biomass based on ninhydrin-reactive nitrogen in extracts of fumigated soils. Soil Biol Biochem 20:107–114CrossRefGoogle Scholar
  3. Assigbetse K, Gueye M, Thioulouse J, Duponnois R (2005) Ectomycorrhizal fungus are not root-growth dependent. Microb Ecol 50:350–359PubMedCrossRefGoogle Scholar
  4. Avrahami S, Liesack W, Conrad R (2003) Effects of temperature and fertilizer on activity and community structure of soil ammonia oxidizers. Environ Microbiol 5:691–705PubMedCrossRefGoogle Scholar
  5. Ba-Diao M (2004) Situation et contraintes des systèmes urbains et périurbains de production horticole et animale dans la région de Dakar. Cahiers Agricultures 13:39–49Google Scholar
  6. Bremner JM (1965) Inorganic forms of nitrogen. In: Black CA, Evans DD, White JL, Endminger LE, Clark FE (eds) Methods in soil analysis. Part 2 agronomy monograph 9. ASA and SSSA, Madison, WI, pp 1179–1237Google Scholar
  7. Brzezinska M, Tiwari SC, Stepniewska Z, Nosalewicz M, Bennicelli RP, Samborska A (2006) Variation of enzyme activities, CO2 evolution and redox potential in an Eutric Histosol irrigated with wastewater and tap water. Biol Fertil Soils 43:131–135CrossRefGoogle Scholar
  8. Cantera JJL, Jordan FL, Stein LY (2006) Effects of irrigation sources on ammonia-oxidizing bacterial communities in a managed turf-covered aridisol. Biol Fertil Soils 43:247–255CrossRefGoogle Scholar
  9. Carr RM, Blumenthal UJ, Mara DD (2004) Guidelines for the safe use of wastewater in agriculture: revisiting WHO guidelines. Water Sci Technol 50:31–38PubMedGoogle Scholar
  10. Cavigellli MA, Robertson GP (2000) The functional significance of denitrifier community composition in a terrestrial ecosystem. Ecology 81:229–241CrossRefGoogle Scholar
  11. Cavigellli MA, Robertson GP (2001) Role of denitrifier diversity in rates of nitrous oxide consumption in a terrestrial ecosystem. Soil Biol Biochem 33:297–310CrossRefGoogle Scholar
  12. Cho JC, Kim SJ (2000) Increase in bacterial community diversity in subsurface aquifers receiving livestock wastewater input. Appl Environ Microbiol 66:956–965PubMedCrossRefGoogle Scholar
  13. Chu H, Fujii T, Morimoto S, Lin X, Yagi K, Hu J, Zhang J (2007) Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil. Appl Environ Microbiol 73:485–491PubMedCrossRefGoogle Scholar
  14. Clark IM, Hirsch PR (2008) Survival of bacterial DNA and culturable bacteria in archived soils from the Rothamsted Broadbalk experiment. Soil Biol Biochem (in press). DOI 10.1016/j.soilbio.2007.11.021
  15. FAO (1998) World reference base for the soil resources (World Soil Resources Report-84) Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  16. Faruqui N, Niang S, Redwood M (2002) Untreated wastewater reuse in market gardens: a case-study of Dakar, Senegal. International Water Management Institute Workshop on Wastewater Use in irrigated agriculture: confronting the livelihood and environmental realities. Hyderabad, IndiaGoogle Scholar
  17. Gaye M, Niang S (2002) Epuration des Eaux Usées et Agriculture Urbaine. Enda, Etudes et Recherche, DakarGoogle Scholar
  18. Geets J, Boon N, Verstraete W (2006) Strategies of aerobic ammonia-oxidizing bacteria for coping with nutrient and oxygen fluctuations. FEMS Microbiol Ecol 58:1–13PubMedCrossRefGoogle Scholar
  19. Gelsomino A, Badalucco L, Ambrosoli R, Crecchio C, Puglisi E, Meli SM (2006) Changes in chemical and biological soil properties as induced by anthropogenic disturbance: a case study of an agricultural soil under recurrent flooding by wastewaters. Soil Biol Biochem 38:2069–2080CrossRefGoogle Scholar
  20. Girvan MS, Campbell CD, Killham K, Prosser JI, Glover LA (2005) Bacterial diversity promotes community stability and functional resilience after perturbation. Environ Microbiol 7:301–313PubMedCrossRefGoogle Scholar
  21. Hastings RC, Ceccherini MT, Miclaus N, Saunders JR, Bazzicalupo M, McCarthy AJ (1997) Direct molecular biological analysis of ammonia oxidising bacteria populations in cultivated soil plots treated with swine manure. FEMS Microbio Ecol 23:45–54CrossRefGoogle Scholar
  22. Heidarpour M, Mostafazadeh-Fard B, Koupai JA, Malekian R (2007) The effects of treated wastewater on soil chemical properties using subsurface and surface irrigation methods. Agric Water Manag 90:87–94CrossRefGoogle Scholar
  23. Jordan FL, Cantera JJL, Fenn ME, Stein LY (2005) Autotrophic ammonia-oxidizing bacteria contribute minimally to nitrification in a nitrogen-impacted forested ecosystem. Appl Environ Microbiol 71:197–206PubMedCrossRefGoogle Scholar
  24. Mbaye A (1999) Production des légumes à Dakar: importance, contraintes et potentialités. In: Smith O (ed) Agriculture urbaine en Afrique de l’Ouest. International Development Research Center, Ohawa, pp 56–66Google Scholar
  25. Meli S, Porto M, Belligno A, Bufo SA, Mazzatura A, Scopa A (2002) Influence of irrigation with lagooned urban wastewater on chemical and microbiological soil parameters in a citrus orchard under Mediterranean condition. Sci Total Environ 285:69–77PubMedCrossRefGoogle Scholar
  26. Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by DGGE analysis of PCR-amplified genes coding for 16S ribosomal RNA. Appl Environ Microbiol 59:695–700PubMedGoogle Scholar
  27. Okano Y, Hristova KR, Leutenegger CM, Jackson LE, Denison RF, Gebreyesus B, Lebauer D, Scow KM (2004) Application of real-time PCR to study effects of ammonium on population size of ammonia-oxidizing bacteria in soil. Appl Environ Microbiol 70:1008–1016PubMedCrossRefGoogle Scholar
  28. Oved T, Shaviv A, Goldrath T, Mandelbaum RT, Minz D (2001) Influence of effluent irrigation on community composition and function of ammonia-oxydizing bacteria in soil. Appl Environ Microbiol 67:3426–3433PubMedCrossRefGoogle Scholar
  29. Pietramellara G, Dal Canto L, Vettori C, Gallori E, Nannipieri P (1997) Effects of air-drying and wetting cycles on the transforming ability of DNA bound on clay minerals. Soil Biol Biochem 29:55–61CrossRefGoogle Scholar
  30. Porteous LA, Seidler RJ, Watrud LS (1997) An improved method for purifying DNA from soil for polymerase chain reaction amplification and molecular ecology applications. Mol Ecol 6:787–791CrossRefGoogle Scholar
  31. Princic A, Mahne I, Megusar F, Paul EA, Tiedje JM (1998) Effects of pH and oxygen and ammonium concentrations on the community structure of nitrifying bacteria from wastewater. Appl Environ Microbiol 64:3584–3590PubMedGoogle Scholar
  32. Prosser JI, Embley TM (2002) Cultivation-based and molecular approaches to characterisation of terrestrial and aquatic nitrifiers. Anton van Leeuwenhoek 81:165–179CrossRefGoogle Scholar
  33. Ramirez-Fuentes E, Lucho-Constantino C, Escamilla-Silva E, Dendooven L (2002) Characteristics, and carbon and nitrogen dynamics in soil irrigated with wastewater for different lengths of time. Bioresource Technol 85:179–187CrossRefGoogle Scholar
  34. Ranjard L, Lejon DPH, Mougel C, Schehrer L, Merdinoglu D, Chaussod R (2003) Sampling strategy in molecular microbial ecology: influence of soil sample size on DNA fingerprinting analysis of fungal and bacterial communities. Environ Microbiol 5:1111–1120PubMedCrossRefGoogle Scholar
  35. Rao MA, Sannino F, Nocerino G, Puglisi E, Gianfreda L (2003) Effect of air-drying treatment on enzymatic activities of soils affected by anthropogenic activities. Biol Fertil Soils 38:327–332CrossRefGoogle Scholar
  36. Rotthauwe JH, Witzel KP, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol 63:4704–4712PubMedGoogle Scholar
  37. Sikora LJ, Stott DE (1996) Soil organic carbon and nitrogen. In: Doran JW, Jones AJ (eds) Methods for assessing soil quality. SSSA special publication No. 49 SSSA, Madison, WI, pp 157–167Google Scholar
  38. Stephen JR, Chang YJ, MacNaughton SJ, Kowalchuk GA, Leung KT, Flemming CA, White DC (1999) Effect of toxic metals on indigenous soil b-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria. Appl Environ Microbiol 65:95–101PubMedGoogle Scholar
  39. Verhagen FJM, Duyts H, Laanbroek HJ (1992) Competition for ammonium between nitrifying and heterotrophic bacteria in continuously percolated soil columns. Appl Environ Microbiol 58:3303–3311PubMedGoogle Scholar
  40. Whiteley AS, Griffiths RI, Bailey MJ (2003) Analysis of the microbial functional diversity within water-stressed soil communities by flow cytometric analysis and CTC+ cell sorting. J Microbiol Methods 54:257–267PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Ndèye Yacine Badiane Ndour
    • 1
    • 2
  • Ezékiel Baudoin
    • 2
    • 4
  • Aliou Guissé
    • 2
    • 3
  • Mountakha Seck
    • 2
    • 3
  • Mamadou Khouma
    • 1
  • Alain Brauman
    • 4
  1. 1.Institut Sénégalais de Recherche AgricoleLaboratoire National de Recherche sur les Productions VégétalesDakarSénégal
  2. 2.IRD-Seqbio, LEMSATDakarSénégal
  3. 3.Département de Biologie VégétaleUniversité Cheikh Anta DiopDakarSénégal
  4. 4.IRD-Seqbio, Montpellier SupAgroMontpellier cedex 1France

Personalised recommendations