Biology and Fertility of Soils

, Volume 44, Issue 5, pp 791–796 | Cite as

Soil engineering ants increase grass root arbuscular mycorrhizal colonization

  • Jens Dauber
  • Robin Niechoj
  • Helmut Baltruschat
  • Volkmar Wolters
Short Communication


The role of edaphic factors in driving the relationship between plant community structure and ecosystem processes is a key issue of the current debate on functional implications of biodiversity. In this study, we draw a direct link between aboveground/belowground relationships, vegetation structure, and aboveground management. We used ground nesting ants and arbuscular mycorrhizal fungi (AMF) as an example for quantifying the role of biotic interactions in soil. Although both groups are known to have a major impact on grasslands, the interactive effect of these taxa on vegetation structure and its sensitivity to grassland management is poorly understood. We show that the ant Lasius flavus increases the root arbuscular mycorrhizal colonization (AMC) of grasses by modifying biotic and abiotic soil properties. As a possible consequence, the shoot length of grass growing on ant mounds was shorter and shoot N and P concentrations were higher than in grass growing off of the mounds. In addition, management affected ant nest architecture and soil and, in turn, AMC. These results emphasize the need to consider the interactions between plants, soil microorganisms, soil fauna, and aboveground management to increase the understanding of the drivers of biodiversity and ecosystem functioning in grasslands both aboveground and belowground.


Belowground species interactions Ecosystem engineering Ecosystem functioning Formicidae Soil biota 



This work was supported by the Deutsche Forschungsgemeinschaft (DFG) in the framework of the Collaborative Research Centre (SFB) 299. We thank S. Hepp for helping with the fieldwork and in the laboratory and W.E. Kunin for making valuable comments on the manuscript.


  1. Baker TJ, Gowen SR (1996) Staining nematodes and arbuscular mycorrhizae in the same root sample. Fundam Appl Nematol 19:607–608Google Scholar
  2. Bardgett RD, Bowman WD, Kaufmann R, Schmidt SK (2005) A temporal approach to linking aboveground and belowground ecology. Trends Ecol Evol 20:634–641PubMedCrossRefGoogle Scholar
  3. Blomqvist MM, Olff H, Blaauw MB, Bongers T, van der Putten WH (2000) Interactions between above- and belowground biota: importance for small-scale vegetation mosaics in a grassland ecosystem. Oikos 90:582–598CrossRefGoogle Scholar
  4. Boulton AM, Amberman KD (2006) How ant nests increase soil biota richness and abundance. Biodivers Conserv 15:69–82CrossRefGoogle Scholar
  5. Dauber J, Wolters V (2000) Microbial activity and functional diversity in the mounds of three different ant species. Soil Biol Biochem 32:93–99CrossRefGoogle Scholar
  6. De Deyn GB, Raaijmakers CE, Zoomer HR, Berg MP, de Ruiter PC, Verhoef HA, Bezemer TM, van der Putten WH (2003) Soil invertebrate fauna enhances grassland succession and diversity. Nature 422:711–713PubMedCrossRefGoogle Scholar
  7. Dostal P (2007) Population dynamics of annuals in perennial grassland controlled by ants and environmental stochasticity. J Veg Sci 18:91–102CrossRefGoogle Scholar
  8. Dostal P, Breznova M, Kozlickova V, Herben T, Kovar P (2005) Ant-induced soil modification and its effect on plant below-ground biomass. Pedobiologia 49:127–137CrossRefGoogle Scholar
  9. Elmes GW (1991) Ant colonies and environmental disturbance. Symp Zool Soc Lond 63:15–32Google Scholar
  10. Endlweber K, Scheu S (2007) Interactions between mycorrhizal fungi and Collembola: effects on root structure of competing plant species. Biol Fertil Soils 43:741–749CrossRefGoogle Scholar
  11. Ettema CH, Wardle DA (2002) Spatial soil ecology. Trends Ecol Evol 17:177–183CrossRefGoogle Scholar
  12. Folgarait PJ (1998) Ant biodiversity and its relationship to ecosystem functioning: a review. Biodivers Conserv 7:1221–1244CrossRefGoogle Scholar
  13. Friese CF, Allen MF (1993) The interaction of harvester ants and vesicular–arbuscular mycorrhizal fungi in a patchy semi-arid environment: the effects of mound structure on fungal dispersion and establishment. Funct Ecol 7:13–20CrossRefGoogle Scholar
  14. Gange AC (1993) Translocation of mycorrhizal fungi by earthworms during early succession. Soil Biol Biochem 25:1021–1026CrossRefGoogle Scholar
  15. Gange A (2000) Arbuscular mycorrhizal fungi, Collembola and plant growth. Trends Ecol Evol 15:369–372PubMedCrossRefGoogle Scholar
  16. Gerike S, Kurmies B (1952) Die kolorimetrische Phosphorsäurebestimmung mit Ammonium-Vanadat-Molybdat und ihre Anwendung in der Pflanzenanalyse. J Plant Nutr Soil Sci 104:235–247Google Scholar
  17. Gormsen D, Olsson PA, Hedlund K (2004) The influence of collembolans and earthworms on AM fungal mycelium. Appl Soil Ecol 27:211–220CrossRefGoogle Scholar
  18. Graham MH (2003) Confronting multicollinearity in ecological multiple regression. Ecology 84:2809–2815CrossRefGoogle Scholar
  19. Harinikumar KM, Bagyaraj DJ (1994) Potential of earthworms, ants, millipedes, and termites for dissemination of vesicular–arbuscular mycorrhizal fungi in soil. Biol Fertil Soils 18:115–118CrossRefGoogle Scholar
  20. Heinemeyer A, Fitter AH (2004) Impact of temperature on the arbuscular mycorrhizal (AM) symbiosis: growth responses of the host plant and its AM fungal partner. J Exp Bot 55:525–534PubMedCrossRefGoogle Scholar
  21. Helgason T, Daniell J, Husband R, Fitter AH, Young JPW (1998) Ploughing up the wood-wide web? Nature 394:431PubMedCrossRefGoogle Scholar
  22. Johnson NC, Zak DR, Tilman D, Pfleger FL (1991) Dynamics of vesicular–arbuscular mycorrhizae during old field succession. Oecologia 86:349–358CrossRefGoogle Scholar
  23. Jouquet P, Dauber J, Lagerlöf J, Lavelle P, Lepage M (2006) Soil invertebrates as ecosystem engineers: intended and accidental effects on soil and feedback loops. Appl Soil Ecol 32:153–164CrossRefGoogle Scholar
  24. Kaspar TC, Ewing RP (1997) ROOTEDGE: software for measuring root length from desktop scanner images. Agron J 89:932–940Google Scholar
  25. King TJ (1981) Ant-hill vegetation in acidic grasslands in the Gower Peninsula, South Wales. New Phytol 88:559–571CrossRefGoogle Scholar
  26. Kovar P, Kovarova M, Dostal P, Herben T (2001) Vegetation of ant-hills in a mountain grassland: effects of mound history and of dominant ant species. Plant Ecol 156:215–227CrossRefGoogle Scholar
  27. Lavelle P, Bignell D, Lepage M, Wolters V, Roger P, Ineson P, Heal OW, Dhillion S (1997) Soil function in a changing world: the role of invertebrate ecosystem engineers. Eur J Soil Biol 33:159–193Google Scholar
  28. Newsham KK, Fitter AH, Watkinson AK (1995) Multi-functionality and biodiversity in arbuscular mycorrhizas. Trends Ecol Evol 10:407–411CrossRefGoogle Scholar
  29. Rillig MC (2004) Arbuscular mycorrhizae and terrestrial ecosystem processes. Ecol Lett 7:740–754CrossRefGoogle Scholar
  30. Rothanzl J, Kotoučová M, Hrabinová I, Plačková I, Herben T (2006) Genetic differentiation of Agrostis capillaris in a grassland system with stable heterogeneity due to terricolous ants. J Ecol 95:197–207CrossRefGoogle Scholar
  31. Schlichting E, Blume H-P, Stahr E (1995) Bodenkundliches Praktikum: eine Einführung in Pedologisches Arbeiten für Ökologen, Insbesondere Land- und Forstwirte und für Geowissenschaftler. Blackwell, BerlinGoogle Scholar
  32. van der Heijden MGA (2004) Arbuscular mycorrhizal fungi as support systems for seedling establishment in grassland. Ecol Lett 7:293–303CrossRefGoogle Scholar
  33. Wardle DA, Bardgett RD, Klironomos JN, Setälä H, van der Putten WH, Wall DH (2004) Ecological linkages between aboveground and belowground biota. Science 304:1629–1633PubMedCrossRefGoogle Scholar
  34. Wright JP, Jones CG (2006) The concept of organisms as ecosystem engineers ten years on: progress, limitations, and challenges. BioScience 56:203–209CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jens Dauber
    • 1
    • 2
  • Robin Niechoj
    • 1
    • 3
  • Helmut Baltruschat
    • 4
  • Volkmar Wolters
    • 1
  1. 1.Department of Animal Ecology IFZUniversity of GiessenGiessenGermany
  2. 2.Institute of Integrative and Comparative BiologyUniversity of LeedsLeedsUK
  3. 3.Department of Life SciencesUniversity of LimerickLimerickIreland
  4. 4.Department of Phytopathology and Applied Zoology IFZUniversity of GiessenGiessenGermany

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