Biology and Fertility of Soils

, Volume 41, Issue 5, pp 371–378 | Cite as

Changes in acetylene reduction activities and effects of inoculated rhizosphere nitrogen-fixing bacteria on rice

  • Zhe PiaoEmail author
  • Zongjun Cui
  • Bin Yin
  • Jian Hu
  • Chunhong Zhou
  • Guanghui Xie
  • Baolin Su
  • Shixue Yin
Short Communication


Acetylene reduction activities (ARAs) of soils and rice plants during rice-growing season were monitored in temperate region in northeast China. This activity was significantly higher in rhizosphere soil than that in inter-row soil after rice seedlings were transplanted. The ARA was high for most of growing season, suggesting that the native N2-fixing bacteria responded to rice roots very quickly. Sixteen strains of free-living N2-fixing bacteria were isolated from three different soils. The ARAs of these strains were correlated with the averaged soil ARAs, suggesting that the isolated strains were likely the active flora responsive to rice roots. The strains were inoculated by soaking seedling roots into the liquid culture for 2 h, and the seedlings were transplanted into pots. Most strains tested did not show any growth-promoting effects except Azotobacter armeniacus and Azotobacter nigricans, which showed growth-promoting effects only at late rice growth stage and only when inoculated in combination but not separately. Present data indicated the promising future applications of these two strains in combination in the region, but further research is needed to understand the underlying mechanisms.


N2-fixing bacteria Plant growth-promoting effects Rhizosphere Acetylene reduction activities Temperate climate region 



Financial supports from National Natural Science Foundation of China (40471072 and 30470060) and the National High Technology Research and Development Program of China (2002AA601012) are acknowledged. Author Zhe Piao is indebted to Prof. M.Y. Cai, Institute of Microbiology, Chinese Academy of Sciences, Beijing, for her identification of the strains and for her helpful discussions on biological nitrogen fixation, Mr. Z.X. Jin and Prof. C.H. Cui (Northeast Agricultural College), Ms. Y.H. Guo (Shenyang Agricultural College), Mr. Y.Z. Sun and Mr. T.Y. Shi (Yanbian Agricultural College) for their help in sampling.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Zhe Piao
    • 1
    • 2
    Email author
  • Zongjun Cui
    • 3
  • Bin Yin
    • 1
  • Jian Hu
    • 2
  • Chunhong Zhou
    • 2
  • Guanghui Xie
    • 3
  • Baolin Su
    • 3
  • Shixue Yin
    • 1
    • 2
  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingPeople’s Republic of China
  2. 2.Microbiology Section, College of Environmental Science and EngineeringYangzhou UniversityYangzhouPeople’s Republic of China
  3. 3.College of Agriculture and BiotechnologyChina Agricultural UniversityBeijingPeople’s Republic of China

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