Biology and Fertility of Soils

, Volume 42, Issue 4, pp 279–285 | Cite as

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

  • Y. Bashan
  • J. J. Bustillos
  • L. A. Leyva
  • J.-P. Hernandez
  • M. Bacilio
Original Paper

Abstract

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.

Keywords

Antheroxanthin Azospirillum Bacterial inoculants β-carotene Lutein Neoxanthin Plant growth-promoting bacteria PGPR Plant pigments Photosynthesis Violaxanthin Xanthophyll Zeaxanthin 

Notes

Acknowledgements

Yoav Bashan participated in this study in memory of the late Messrs. Avner and Uzi Bashan from Israel. We thank Francisco Hernandez of CIBNOR for HPLC analysis of pigments and the staff editor for improving the English text. This study was partially supported by The National Council of Science and Technology of Mexico (CONACYT contract U39520-Z) and The Bashan Foundation, USA.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Y. Bashan
    • 1
  • J. J. Bustillos
    • 1
  • L. A. Leyva
    • 1
  • J.-P. Hernandez
    • 1
  • M. Bacilio
    • 1
  1. 1.Environmental Microbiology GroupCenter for Biological Research of the Northwest (CIBNOR)La PazMexico

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