Biology and Fertility of Soils

, Volume 35, Issue 5, pp 387–389 | Cite as

Efficacy of three 15N labelling techniques for estimating below-ground N in Sesbania rostrata

  •  P. Chalk
  •  J. Ladha
  •  A. Padre
Short Communication

Abstract.

Three 15N labelling strategies for estimating below-ground N in Sesbania rostrata grown in pots under flooded conditions were examined. The estimated proportions of total plant N resident below-ground were 13% (stem injection), 42% (leaf immersion) and 56% (adventitious root feeding). The average estimate based on leaf and root immersion (49%) was considered to be realistic on the basis of published literature with other foliar labelled legumes, while root and root-derived N appeared to be underestimated by stem injection.

Adventitious root labelling Foliar labelling 15N Stem injection 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  P. Chalk
    • 1
  •  J. Ladha
    • 1
  •  A. Padre
    • 1
  1. 1.Crop, Soil and Water Sciences Division, International Rice Research Institute, DAPO Box 7777, Metro-Manila, Philippines

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