Geo-Marine Letters

, Volume 30, Issue 3–4, pp 411–425 | Cite as

Geochemical and microbiological characteristics of sediments near the Malenky mud volcano (Lake Baikal, Russia), with evidence of Archaea intermediate between the marine anaerobic methanotrophs ANME-2 and ANME-3

  • Tamara I. Zemskaya
  • Tatiayna V. Pogodaeva
  • Olga V. Shubenkova
  • Svetlana M. Сhernitsina
  • Olga P. Dagurova
  • Savelii P. Buryukhaev
  • Bair B. Namsaraev
  • Oleg M. Khlystov
  • Aleksandr V. Egorov
  • Aleksei A. Krylov
  • Gennadii V. Kalmychkov
Original
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Abstract

Detailed lithological, biogeochemical and molecular biological analyses of core sediments collected in 2002–2006 from the vicinity of the Malenky mud volcano, Lake Baikal, reveal considerable spatial variations in pore water chemical composition, with total concentrations of dissolved salts varying from 0.1 to 1.8‰. Values of methane δ13С in the sediments suggest a biogenic origin (δ13Сmin. −61.3‰, δ13Сmax. −72.9‰). Rates of sulphate reduction varied from 0.001 to 0.7 nmol cm−3 day−1, of autotrophic methanogenesis from 0.01 to 2.98 nmol CH4 cm−3 day−1, and of anaerobic oxidation of methane from 0 to 12.3 nmol cm−3 day−1. These results indicate that methanogenic processes dominate in gas hydrate-bearing sediments of Lake Baikal. Based on clone libraries of 16S rRNA genes amplified with Bacteria- and Archaea-specific primers, investigation of microbial diversity in gas hydrate-bearing sediments revealed bacterial 16S rRNA clones classified as Deltaproteobacteria, Gammaproteobacteria, Chloroflexi and OP11. Archaeal clone sequences are related to the Crenarchaeota and Euryarchaeota. Baikal sequences of Archaea form a distinct cluster occupying an intermediate position between the marine groups ANME-2 and ANME-3 of anaerobic methanotrophs.

Keywords

Pore Water Archaea Methanosaeta Sulphate Reduction Rate Methane Hydrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

This work was supported by the Presidium of the Russian Academy of Sciences, Program 17 (Project 17.9), and the Russian Foundation for Basic Research Projects Nos. 08-05-00709-а, Integration Project No. 27.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Tamara I. Zemskaya
    • 1
  • Tatiayna V. Pogodaeva
    • 1
  • Olga V. Shubenkova
    • 1
  • Svetlana M. Сhernitsina
    • 1
  • Olga P. Dagurova
    • 2
  • Savelii P. Buryukhaev
    • 2
  • Bair B. Namsaraev
    • 2
  • Oleg M. Khlystov
    • 1
  • Aleksandr V. Egorov
    • 3
  • Aleksei A. Krylov
    • 4
  • Gennadii V. Kalmychkov
    • 5
  1. 1.Limnological InstituteSiberian Branch of Russian Academy of SciencesIrkutskRussia
  2. 2.Institute of General and Experimental BiologySiberian Branch of Russian Academy of SciencesUlan-UdeRussia
  3. 3.Institute of Oceanology, Russian Academy of SciencesMoscowRussia
  4. 4.VNIIOkeangeologiaSt. PetersburgRussia
  5. 5.Vinogradov Institute of GeochemistrySiberian Branch of Russian Academy of SciencesIrkutskRussia

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