Journal of Comparative Physiology B

, Volume 187, Issue 5–6, pp 869–879 | Cite as

A dramatic blood plasticity in hibernating and 14-day hindlimb unloading Daurian ground squirrels (Spermophilus dauricus)

  • Huan-Xin Hu
  • Fang-Ying Du
  • Wei-Wei Fu
  • Shan-Feng Jiang
  • Jin Cao
  • Shen-Hui Xu
  • Hui-Ping Wang
  • Hui Chang
  • Nandu Goswami
  • Yun-Fang Gao
Original Paper

Abstract

We compared the effects of hibernation inactivity and 14-day hindlimb unloading in non-hibernating period on biochemical, rheological, and hematological parameters of blood in Daurian ground squirrels (Spermophilus dauricus). Twenty-four squirrels were randomly divided into four groups: control (CON), hibernation (HIB), post-hibernation (POST), and 14-day hindlimb unloading (HU). The results showed that serum enzymes (l-lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase) activities decreased in HIB, POST, and HU squirrels compared with CON. Total protein (including albumin and globulin) maintained in HIB but decreased in HU compared with CON. Total cholesterol and high-density lipoprotein–cholesterol increased in HIB but maintained in HU and POST compared with CON. Meanwhile, serum creatinine decreased and urea increased in HU compared with CON. All blood ions concentrations were unchanged in HIB, POST, and HU squirrels compared with CON except calcium which increased in HIB compared with CON, and phosphorus which increased in HIB and POST compared with CON. Most of detected serum biochemical analytes in POST recovered to the CON level. Blood viscosity, which was unchanged in all shear rates in HU, increased in HIB and recovered in POST in lower shear rates compared with CON. Erythrocyte and corpuscular volume decreased in HIB and HU but maintained in POST compared with CON. All the routine hematological parameters recovered in POST as compared with CON except platelet, which decreased in HIB and POST but maintained in HU compared with CON. In conclusion, our results suggested a remarkable ability to maintain blood homeostasis in hibernating squirrels.

Keywords

Hibernation Disuse Blood biochemical analytes Rheology Routine hematological parameters 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Huan-Xin Hu
    • 1
  • Fang-Ying Du
    • 1
    • 2
  • Wei-Wei Fu
    • 1
  • Shan-Feng Jiang
    • 1
  • Jin Cao
    • 1
  • Shen-Hui Xu
    • 1
  • Hui-Ping Wang
    • 1
  • Hui Chang
    • 1
  • Nandu Goswami
    • 3
  • Yun-Fang Gao
    • 1
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University)Ministry of EducationXi’anChina
  2. 2.Department of Genetics and Molecular Biology, School of MedicineXi’an Jiaotong UniversityXi’anChina
  3. 3.Gravitational Physiology and Medicine Research Unit, Institute of Physiology, Center of Physiological MedicineMedical University GrazGrazAustria

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