Does diet influence salivary enzyme activities in elephant species?
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Asian elephants (Elephas maximus) and African elephants (Loxodonta africana) are herbivore generalists; however, Asian elephants might ingest a higher proportion of grasses than Africans. Although some studies have investigated nutrition-specific morphological adaptations of the two species, broader studies on salivary enzymes in both elephant species are lacking. This study focuses on the comparison of salivary enzymes activity profiles in the two elephant species; these enzymes are relevant for protective and digestive functions in humans. We aimed to determine whether salivary amylase (sAA), lysozyme (sLYS), and peroxidase (sPOD) activities have changed in a species-specific pattern during evolutionary separation of the elephant genera. Saliva samples of 14 Asian and eight African elephants were collected in three German zoos. Results show that sAA and sLYS are salivary components of both elephant species in an active conformation. In contrast, little to no sPOD activity was determined in any elephant sample. Furthermore, sAA activity was significantly higher in Asian compared with African elephants. sLYS and sPOD showed no species-specific differences. The time of food provision until sample collection affected only sAA activity. In summary, the results suggest several possible factors modulating the activity of the mammal-typical enzymes, such as sAA, sLYS, and sPOD, e.g., nutrition and sampling procedure, which have to be considered when analyzing differences in saliva composition of animal species.
KeywordsElephant Salivary enzyme activity Amylase Nutrition Evolution
For the acquisition of saliva samples from Zoo Dresden GmbH, we would like to thank Ronny Moche and David Lück, also Thomas Brockmann, Dr. Dimitri Widmer, and Dr. Eva Ziemssen for their help and support. We would like to thank Sandra Reichler, Dr. Melanie Oesterwind, and the animal caretaker Stefan Geretschläger and his team from Zoo Heidelberg GmbH. From Tierpark Berlin-Friedrichsfelde GmbH, we would like to acknowledge Dr. Günther Strauss and Mario Hammerschmidt and his team for their collaboration. For reviewing and giving helpful annotations and suggestions, we would like to thank Prof. Dr. Marcus Clauss and one anonymous reviewer. We would like to acknowledge Pamela Heidi Douglas for improving the language of this manuscript.
Compliance with ethical standards
This article does not include studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The animals did not experience pain or suffering, and were not distressed by the saliva collection procedure. Therefore, the saliva collection is not an animal experiment according to German law.
Conflict of interest
The authors declare that they have no conflict of interest.
- Barabash RD, Gukevich EK, Berezovskaia ZV et al (1979) Role of peroxidase in the pathogenesis of parodontosis. Vopr meditsinskoi Khimii 25:333–342Google Scholar
- Blanc JJ, Barnes RFW, Craig CG, et al (2007) African elephant status report 2007: an update from the African elephant database. no. 33. Occas. Pap. IUCN Species Surviv. Comm. IUCNSSC Afr. Elephant Spec. GroupGoogle Scholar
- Green JL (1995) The use of lysozyme in winemaking: the interaction of lysozyme with wine and efficacy in preventing malolactic fermentation in Oregon Pinot noir and Chardonnay. Master Thesis. Oregon State UniversityGoogle Scholar
- Guy PR (1976) The feeding behaviour of elephant (Loxodonta africana) in the Sengwa area, Rhodesia. South Afr J Wildl Res 6:55–63Google Scholar
- IUCN (2008) Elephas maximus: Choudhury A, Lahiri Choudhury DK, Desai A, Duckworth JW, Easa PS, Johnsingh AJT, Fernando P, Hedges S, Gunawardena M, Kurt F, Karanth U, Lister A, Menon V, Riddle H, Rübel A; Wikramanayake E (IUCN SSC Asian Elephant Specialist Group): The IUCN Red List of Threatened Species 2008: e.T7140A12828813Google Scholar
- Joshi R, Singh R (2008) Feeding behaviour of wild Asian elephants (Elephas maximus) in the Rajaji National Park. J Am Sci 4:34–48Google Scholar
- Mason G, Rushen J (eds) (2006) Stereotypic animal behaviour: fundamentals and applications to welfare, 2nd edn. CABI Pub, Wallingford, UK, CambridgeGoogle Scholar
- Mau M, Kaiser TM, Südekum K-H (2013) Pilot study on binding of bovine salivary proteins to grit silicates and plant phytoliths. Zool Res 34:87–92Google Scholar
- Mohapatra KK, Patra AK, Paramanik DS (2013) Food and feeding behaviour of Asiatic elephant (Elephas maximus Linn.) in Kuldiha Wild Life Sanctuary, Odisha, India. J Environ Biol Acad Environ Biol India 34:87–92Google Scholar
- Oberg SG, Izutsu KT, Truelove EL (1982) Human parotid saliva protein composition: dependence on physiological factors. Am J Physiol-Gastrointest Liver Physiol 242:G231–G236Google Scholar
- Robbins CT (1983) Wildlife feeding and nutrition. Academic Press, New York, p 235Google Scholar
- Rodiek A (2010) Optimizing different hay types for horses: what have we learned? Proceedings, 2010 California Alfalfa & Forage Symposium and Corn/Cereal Silage Mini-Symposium. Visalia, CAGoogle Scholar
- Shipley LA (1999) Grazers and browsers: how digestive morphology affects diet selection. In: Launchbaugh KL, Sanders KD, Mosley JC (eds) Grazing behavior of livestock and wildlife. Presented in: “Grazing Behavior of Livestock and Wildlife.” 1999. Idaho Forest, Wildlife & Range Exp. Sta. Bull. 70, Univ. of Idaho, Moscow, ID, pp 20–27Google Scholar
- Sukumar R (1989) The Asian elephant: ecology and management. Cambridge studies in applied ecology and resource management. Cambridge University Press, CambridgeGoogle Scholar
- Ullrey DE, Crissey SD, Hintz HF (1997) Elephants: nutrition and dietary husbandry. Nutrition Advisory Group. Michigan State University. http://wildpro.twycrosszoo.org/000ADOBES/Elephants/D297nutrdietEle_NAG.pdf. Accessed 23 July 2015
- van Sonsbeek GR, van der Kolk JH, van Leeuwen JPTM et al (2013) Effect of calcium and cholecalciferol supplementation on several parameters of calcium status in plasma and urine of captive Asian (Elephas maximus) and African elephants (Loxodonta africana). J Zoo Wildl Med Off Publ Am Assoc Zoo Vet 44:529–540. doi: 10.1638/2010-0123R4.1 Google Scholar
- Wing LD, Buss IO (1970) Elephants and forests. Wildl Monogr 19:3–92Google Scholar