Journal of Comparative Physiology B

, Volume 184, Issue 5, pp 589–599 | Cite as

Development and partial characterisation of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis

  • Erin L. Damsteegt
  • Hiroko Mizuta
  • Yuichi Ozaki
  • Naoshi Hiramatsu
  • Takashi Todo
  • Akihiko Hara
  • Shigeho Ijiri
  • Shinji Adachi
  • P. Mark Lokman
Original Paper


Despite its key role in transportation of triacylglycerides in blood, the distribution, localisation and molecular weight variants of apolipoprotein B (Apob) in teleost fish have essentially escaped study. To address this, a specific short-finned eel (Anguilla australis) Apob antiserum was produced by an immunised rabbit, purified and partially characterised. Localisation of Apob at both the mRNA (in situ hybridisation) and protein (immunohistochemistry) levels mirrored that of mammals; thus immunostaining was confined to the interstitial spaces of the liver and the vascular core of the intestinal villi. Immunostaining of proteins by Western blotting, followed by high-resolution LC–MS, indicated that peptide sequence coverage of Apob in low-density lipoproteins spanned the full-length protein. We conclude that only full-length Apob is produced by eels and that both liver and intestine are key sites for its synthesis.


Apolipoprotein B Antiserum Anguilla australis In situ hybridisation Immunohistochemistry 





Apolipoprotein B


5-Bromo-4-chloro-3-indolyl phosphate


Coomassie brilliant blue






Eel elongation factor one alpha


Isopropyl β-d-1-thiogalactopyranoside


Liquid chromatography–mass spectrometry


Low-density lipoprotein


4-Nitro blue tetrazolium chloride


Phosphate-buffered saline




Polyvinylidene fluoride


Quantitative polymerase chain reaction


Sodium dodecyl sulphate polyacrylamide gel electrophoresis




Very low-density lipoprotein


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Erin L. Damsteegt
    • 1
  • Hiroko Mizuta
    • 2
  • Yuichi Ozaki
    • 1
  • Naoshi Hiramatsu
    • 2
  • Takashi Todo
    • 2
  • Akihiko Hara
    • 2
  • Shigeho Ijiri
    • 2
  • Shinji Adachi
    • 2
  • P. Mark Lokman
    • 1
  1. 1.Department of ZoologyUniversity of OtagoDunedinNew Zealand
  2. 2.Faculty of Fisheries SciencesHokkaido UniversityHakodateJapan

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