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Journal of Comparative Physiology B

, Volume 180, Issue 1, pp 105–116 | Cite as

Identification of genes differentially expressed by calorie restriction in the rotifer (Brachionus plicatilis)

  • Aung Kyaw Swar Oo
  • Gen Kaneko
  • Makoto Hirayama
  • Shigeharu Kinoshita
  • Shugo WatabeEmail author
Original Paper

Abstract

A monogonont rotifer Brachionus plicatilis has been widely used as a model organism for physiological, ecological studies and for ecotoxicology. Because of the availability of parthenogenetic mode of reproduction as well as its versatility to be used as live food in aquaculture, the population dynamic studies using the rotifer have become more important and acquired the priority over those using other species. Although many studies have been conducted to identify environmental factors that influence rotifer populations, the molecular mechanisms involved still remain to be elucidated. In this study, gene(s) differentially expressed by calorie restriction in the rotifer was analyzed, where a calorie-restricted group was fed 3 h day−1 and a well-fed group fed ad libitum. A subtracted cDNA library from the calorie-restricted rotifer was constructed using suppression subtractive hybridization (SSH). One hundred sixty-three expressed sequence tags (ESTs) were identified, which included 109 putative genes with a high identity to known genes in the publicly available database as well as 54 unknown ESTs. After assembling, a total of 38 different genes were obtained among 109 ESTs. Further validation of expression by semi-quantitative reverse transcription-PCR showed that 29 out of the 38 genes obtained by SSH were up regulated by calorie restriction.

Keywords

Monogonont rotifer Brachionus plicatilis Calorie restriction Suppression subtractive hybridization Gene expression 

Abbreviations

14-3-3

Multifunctional 14-3-3 family chaperone

16S rRNA

Mitochondrial 16S rRNA

BCCIPβ

BRCA2 and CDKN1A-interacting protein, isoform BCCIP beta

CaM64B

Calmodulin (synthetic construct)

CDT1

CDT1 protein

CHP

Conserved hypothetical protein

Dnahc3

Dynein heavy chain domain 3

EBF

EBF protein

EF-1α

Elongation factor-1α

Gale

Galactose-4-epimerase, UDP

Glb

β-galactosidase

Glase

Glycogen phosphorylase

IPS2

2-Isopropylmalate synthase

Irp

Iron regulatory protein

Lis1

Lissencephaly-1

Msh6p

Mismatch repair protein in mitosis and meiosis

NSUN2

NOL1/NOP2/Sun domain family 2 protein

Phm

Peptidylglycine-hydroxylating monooxygenase

PK-TRP

Serine/threonine protein kinase with TRP repeats

Pole

DNA polymerase epsilon

Pols

DNA polymerase sigma

SDHD

Succinate dehydrogenase complex subunit D

Ser

Serine protease

stom

Stom protein

STPP

Serine/threonine phosphatase

TFPI

Tissue factor pathway inhibitor

Tsase

Transposase

tub

Beta 2 tubulin

Znf

Zinc finger protein

Notes

Acknowledgments

Brachionus plicatilis Ishikawa strain used in this study was kindly provided by Professor Atsushi Hagiwara, Faculty of Fisheries, Nagasaki University, Japan. This work was funded in part by Grant-in-Aids for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan and from the Japan Society for Promotion of Science.

Supplementary material

360_2009_389_MOESM1_ESM.pdf (100 kb)
Supplementary Table 1 (PDF 100 kb)
360_2009_389_MOESM2_ESM.pdf (89 kb)
Supplementary Table 2 (PDF 89 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Aung Kyaw Swar Oo
    • 1
  • Gen Kaneko
    • 1
  • Makoto Hirayama
    • 1
    • 2
  • Shigeharu Kinoshita
    • 1
  • Shugo Watabe
    • 1
    Email author
  1. 1.Department of Aquatic Bioscience, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyoJapan
  2. 2.Graduate School of Biosphere ScienceHiroshima UniversityHigashi-HiroshimaJapan

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