Journal of Comparative Physiology B

, Volume 179, Issue 7, pp 897–902 | Cite as

Simultaneous freeze tolerance and avoidance in individual fungus gnats, Exechia nugatoria

  • Todd Sformo
  • F. Kohl
  • J. McIntyre
  • P. Kerr
  • J. G. Duman
  • B. M. Barnes
Original Paper

Abstract

Freeze tolerance and freeze avoidance are typically described as mutually exclusive strategies for overwintering in animals. Here we show an insect species that combines both strategies. Individual fungus gnats, collected in Fairbanks, Alaska, display two freezing events when experimentally cooled and different rates of survival after each event (mean ± SEM: −31.5 ± 0.2°C, 70% survival and −50.7 ± 0.4°C, 0% survival). To determine which body compartments froze at each event, we dissected the abdomen from the head/thorax and cooled each part separately. There was a significant difference between temperature levels of abdominal freezing (−30.1 ± 1.1°C) and head/thorax freezing (−48.7 ± 1.3°C). We suggest that freezing is initially restricted to one body compartment by regional dehydration in the head/thorax that prevents inoculative freezing between the freeze-tolerant abdomen (71.0 ± 0.8% water) and the supercooled, freeze-sensitive head/thorax (46.6 ± 0.8% water).

Keywords

Mycetophilidae Exechia nugatoria Supercooling Exotherm 

Abbreviations

SCP1

Supercooling point 1

SCP2

Supercooling point 2

RH

Relative humidity

WC

Water content

Notes

Acknowledgments

This study was supported by National Science Foundation grants IOB06-18436 to BMB and IOB06-18342 to JGD. We wish to thank B. Tudor, K. Walters (Notre Dame), D. Wagner (UAF) for use of scales, G. Juday (UAF) and R. Lee (Miami University, Ohio) for critical discussions, and D. Sikes (UAF Museum) for initial insect identification.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Todd Sformo
    • 1
  • F. Kohl
    • 1
  • J. McIntyre
    • 2
  • P. Kerr
    • 3
  • J. G. Duman
    • 4
  • B. M. Barnes
    • 1
  1. 1.Institute of Arctic BiologyUniversity of Alaska FairbanksFairbanksUSA
  2. 2.Department of Mathematics and StatisticsUniversity of Alaska FairbanksFairbanksUSA
  3. 3.Plant Pest Diagnostics Branch, California State Collection of ArthropodsCalifornia Department of Food and AgricultureSacramentoUSA
  4. 4.Department of Biological SciencesUniversity of Notre DameNotre DameINUSA

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