Journal of Comparative Physiology B

, Volume 176, Issue 1, pp 1–15 | Cite as

Insect chitin synthases: a review

Review

Abstract

Chitin is the most widespread amino polysaccharide in nature. The annual global amount of chitin is believed to be only one order of magnitude less than that of cellulose. It is a linear polymer composed of N-acetylglucosamines that are joined in a reaction catalyzed by the membrane-integral enzyme chitin synthase, a member of the family 2 of glycosyltransferases. The polymerization requires UDP–N-acetylglucosamines as a substrate and divalent cations as co-factors. Chitin formation can be divided into three distinct steps. In the first step, the enzymes‘ catalytic domain facing the cytoplasmic site forms the polymer. The second step involves the translocation of the nascent polymer across the membrane and its release into the extracellular space. The third step completes the process as single polymers spontaneously assemble to form crystalline microfibrils. In subsequent reactions the microfibrils combine with other sugars, proteins, glycoproteins and proteoglycans to form fungal septa and cell walls as well as arthropod cuticles and peritrophic matrices, notably in crustaceans and insects. In spite of the good effort by a hardy few, our present knowledge of the structure, topology and catalytic mechanism of chitin synthases is rather limited. Gaps remain in understanding chitin synthase biosynthesis, enzyme trafficking, regulation of enzyme activity, translocation of chitin chains across cell membranes, fibrillogenesis and the interaction of microfibrils with other components of the extracellular matrix. However, cumulating genomic data on chitin synthase genes and new experimental approaches allow increasingly clearer views of chitin synthase function and its regulation, and consequently chitin biosynthesis. In the present review, I will summarize recent advances in elucidating the structure, regulation and function of insect chitin synthases as they relate to what is known about fungal chitin synthases and other glycosyltransferases.

Keywords

Chitin Chitin synthesis Chitin synthase Cuticle Glycosyltransferase Insect Peritrophic matrix Manduca sexta 

Notes

Acknowledgements

The author is grateful to Drs. Klaus Beyenbach and Helmut Wieczorek for critically reading the manuscript and also likes to thank Lars Zimoch, Ulla Mädler and Margret Düvel for the excellent cooperation. This work was supported by the Deutsche Forschungsgemeinschaft (grants Me2029/1-2 and SFB 431).

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Biology/Chemistry, Division of Animal PhysiologyUniversity of OsnabrückOsnabruckGermany

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