Journal of Comparative Physiology B

, Volume 175, Issue 2, pp 133–145

Dynamics of blood chylomicron fatty acids in a marine carnivore: implications for lipid metabolism and quantitative estimation of predator diets

  • Margaret H. Cooper
  • Sara J. Iverson
  • Horacio Heras
Article

Abstract

Blubber fatty acid(s) (FA) signatures can provide accurate estimates of predator diets using quantitative FA signature analysis, provided that aspects of predator FA metabolism are taken into account. Because the intestinal absorption of dietary FA and their incorporation into chylomicrons (the primary transport lipoproteins for dietary FA in the blood) may influence the relationship between FA composition in the diet and adipose tissue, we investigated the metabolism of individual FA at these early stages of assimilation. We also investigated the capacity of chylomicron signatures to provide quantitative estimates of prey composition of an experimental meal. Six captive juvenile grey seals (Halichoerus grypus) were fed either 2.3 kg (n=3) or 4.6 kg (n=3) of Atlantic herring (Clupea harengus). Although chylomicron FA signatures resembled diet signatures at all samplings, absolute differences were smallest at 3-h post-feeding, when chylomicrons were likely largest and had the greatest ratio of triacylglycerol to phospholipid FA. Specific FA that differed significantly between diet and chylomicron signatures reflected either input from endogenous sources or loss through peroxisomal β-oxidation. When these aspects of metabolism were accounted for, the quantitative predictions of diet composition generated using chylomicron signatures were extremely accurate, even when tested against 28 other prey items.

Keywords

Fatty acids Chylomicrons Pinnipeds Diet 

Abbreviations

FA

Fatty acid(s)

FAME

Fatty acid methyl ester(s)

K-L

Kulback-Leibler

MUFA

Monounsaturated fatty acid(s)

PL

Phospholipid(s)

PUFA

Polyunsaturated fatty acid(s)

QFASA

Quantitative fatty acid signature analysis

SFA

Saturated fatty acid(s)

TAG

Triacylglycerol(s)

VLDL

Very low-density lipoproteins

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Margaret H. Cooper
    • 1
  • Sara J. Iverson
    • 1
  • Horacio Heras
    • 2
  1. 1.Department of BiologyDalhousie UniversityHalifaxCanada
  2. 2.Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONCET-UNLP, Fac. MedicinaUniversidad Nacional de La PlataLa PlataArgentina

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