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Journal of Comparative Physiology B

, Volume 174, Issue 1, pp 1–12 | Cite as

Physiological adaptations of the raccoon dog (Nyctereutes procyonoides) to seasonal fasting-fat and nitrogen metabolism and influence of continuous melatonin treatment

  • A.-M. Mustonen
  • P. Nieminen
  • M. Puukka
  • J. Asikainen
  • S. Saarela
  • S.-L. Karonen
  • J. V. K. Kukkonen
  • H. Hyvärinen
Original Paper

Abstract

The raccoon dog (Nyctereutes procyonoides) is a middle-sized canid with profound autumnal fattening followed by winter sleep. This study investigated the effects of prolonged fasting-induced winter sleep on the fat and nitrogen metabolism of the species. Half of the animals were treated with continuous-release melatonin implants to induce artificial short photoperiod. Autumnal accumulation of fat was characterized by low plasma free fatty acid (FFA), diacylglycerol (DG), and triacylglycerol (TG) levels. After transition to winter catabolism, the circulating lipid levels increased due to enhanced lipolysis. Two months of fasting resulted in a steady 3.1 kg weight loss (28% of body mass, 0.47% day−1). Storage fat was mobilized during the winter sleep reflected by the elevated FFA and DG concentrations. The lowered insulin levels could be a stimulator for TG hydrolysis. The plasma total amino acid concentrations, urea levels, and urea-creatinine ratios decreased due to fasting, whereas ammonia and total protein concentrations remained stable. The effects of melatonin on energy metabolism were modest. The results indicate that the raccoon dog is well adapted to long-term wintertime fasting utilizing fat as the principal metabolic fuel. The species can maintain its protein catabolism constant for at least 60 days. Decreased cortisol and thyroid hormone concentrations may contribute to protein sparing.

Key words

Fasting Lipids Nitrogen metabolism Nyctereutes procyonoides Raccoon dog 

Abbreviations

AA

amino acids

α-AB

α-aminobutyrate

Ala

alanine

ANOVA

analysis of variance

Arg

arginine

BM

body mass

BMI

body mass index

Chol

cholesterol

Cit

citrulline

DG

diacylglycerols

FFA

free fatty acids

GH

growth hormone

Gln

glutamine

Gly

glycine

3-MH

3-methylhistidine

MR

metabolic rate

NH3

ammonia

Orn

ornithine

PC

phosphatidylcholine

Phe

phenylalanine

PL

phospholipids

Pro

proline

Ser

serine

SM

sphingomyelin

T3

triiodothyronine

T4

thyroxine

Ta

ambient temperature

TAA

total amino acids

Tb

body temperature

TG

triacylglycerols

TL

total lipids

TP

total protein

U/C

urea–creatinine

WAT

white adipose tissue

Notes

Acknowledgements

We thank Mrs. Anita Kervinen for laboratory analyses and Mr. Kasper Heikkilä for technical assistance. Financial support was provided by the Helve Foundation and the Faculty of Science of the University of Joensuu. This experiment complies with the current laws of Finland.

Supplementary material

Supplement 1 + 2

supplements1-2.pdf (30 kb)
(PDF 30 KB)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • A.-M. Mustonen
    • 1
  • P. Nieminen
    • 1
  • M. Puukka
    • 2
  • J. Asikainen
    • 1
  • S. Saarela
    • 3
  • S.-L. Karonen
    • 4
  • J. V. K. Kukkonen
    • 1
  • H. Hyvärinen
    • 1
  1. 1.Department of BiologyUniversity of JoensuuJoensuuFinland
  2. 2.Department of Clinical ChemistryUniversity of OuluOuluFinland
  3. 3.Department of BiologyUniversity of OuluOuluFinland
  4. 4.Department of Clinical ChemistryBiomedicum HelsinkiHelsinkiFinland

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