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Overexpression of isoform B of Dgp-1 gene enhances locomotor activity in senescent Drosophila males and under heat stress

  • Sergey A. FedotovEmail author
  • Natalia G. Besedina
  • Julia V. Bragina
  • Larisa V. Danilenkova
  • Elena A. Kamysheva
  • Nikolai G. Kamyshev
Original Paper

Abstract

Here, we describe the longevity and locomotor behavior of senescent Drosophila males with altered expression of Dgp-1 gene. In comparison with the wild-type Canton-S (CS) males, six characteristics of the phenotype of Dgp-1[843k] mutant were found: (1) low expression of isoform A; (2) augmented expression of isoform B; (3) reduction in the mean lifespan; (4) decrease in the running speed in 3-day-old flies; (5) maintenance of a high run frequency in senescent flies; and (6) resistance to heat stress manifested as maintenance of a high run frequency at 29 °C. After cessation of “cantonization” process, mean lifespan of the mutant males drifted from low to high values finally exceeding that for CS. In contrast, behavioral phenotype of the mutant was robust. Using the GAL4/UAS system, we showed that neurospecific overexpression of isoform B resulted in a slight decrease of longevity and a high level of run frequency in the senescent flies, similar to that in Dgp-1[843K] mutant. In addition, a decreased level of reactive oxygen species was found in Dgp-1[843K] mutant males maintained under stress conditions. The elevated resistance to oxidative stress probably explains the two distinctive features of the mutation: resistance to aging processes and thermal stress displayed at behavioral level.

Keywords

Locomotion Heat stress Senescence Oxidative stress Drosophila Dgp-1 mutant 

Abbreviations

ARLI

Age-related locomotor impairments

CI

Confidence intervals

CS

Canton-S

GWAS

Genome-wide association study

IIS

Insulin/insulin-like growth factor signaling

ROS

Reactive oxygen species

Notes

Acknowledgements

We thank the Bloomington Drosophila Stock Center at Indiana University (USA) and the Vienna Drosophila Resource Center at Campus Science Support Facilities (Austria) for providing us with transgenic GAL4 and RNAi fly stocks. We are grateful to Dr. Konstantin G. Iliadi (The Hospital for Sick Children, Toronto, ON, Canada) for propagation, maintenance, and transcontinental shipment of these stocks to our laboratory. In addition, our gratitude is to Center “Biocollection” at Pavlov Institute of Physiology for help in the maintenance of Drosophila lines. Many thanks to anonymous reviewer who prompted us to consider sleep as a possible target of Dgp-1[843K] mutation and corrected our English language in the first version of the manuscript.

Funding

This work was supported by the project Mega_SPbU_2013—6 from St. Petersburg State University, the Postdoctoral fellowship program (ID = 34799261) of personnel support for research conducted under the guidance of leading scientists of St. Petersburg State University, the Russian Program of fundamental researches (2013–2020, GP-14, section 63). The funding sources had no involvement in any aspects of the study.

Compliance with ethical standards

Conflict of interest

Sergey A. Fedotov, Natalia G. Besedina, Julia V. Bragina, Larisa V. Danilenkova, Elena A. Kamysheva, and Nikolai G. Kamyshev declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or vertebrates performed by any of the authors.

Informed consent

For this type of study, formal consent is not required.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Amyloid BiologySt. Petersburg State UniversitySaint PetersburgRussia
  2. 2.Laboratory of Comparative Behavioral GeneticsPavlov Institute of Physiology of the Russian Academy of SciencesSaint PetersburgRussia

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