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Journal of Comparative Physiology A

, Volume 204, Issue 9–10, pp 801–810 | Cite as

Effects of pars intercerebralis removal on circatidal rhythm in the mangrove cricket, Apteronemobius asahinai

  • Hiroki Takekata
  • Hideharu Numata
  • Sakiko Shiga
Original Paper

Abstract

The circatidal rhythm is an endogenous rhythm corresponding to the tidal cycles, and its neural mechanism remains unknown. The mangrove cricket, Apteronemobius asahinai, possesses both circatidal and circadian clocks, and simultaneously exhibits circatidal and circadian rhythms in its locomotor activity. In a previous study, we showed that surgical removal of the optic lobes, the principal circadian clock locus in crickets, disrupted their circadian rhythm, but not their circatidal rhythm. In this study, we focused on the pars intercerebralis (PI) because surgical removal of the PI disrupts the circadian rhythm and causes arrhythmic activity in some cricket species. After surgical removal of the PI, the proportion of crickets displaying circatidal rhythm decreased, and more than half of the crickets exhibited arrhythmic activity. Surgical removal of the regions around the PI also caused a similar effect on locomotor activity. Our results indicate that the PI and/or its surrounding regions are important not only for circadian but also for circatidal rhythm. This suggests the presence of a neural or hormonal pathway in the PI and/or its surrounding regions that is common to the circatidal and circadian rhythms.

Keywords

Circatidal clock Circadian clock Pars intercerebralis Neurosecretory cells Locomotor activity rhythm 

Abbreviations

Dorsal

Bilateral dorsal perikarya excluding the PI

PAF

Paraldehyde-fuchsin

Medial

Mediodorsal perikarya including the PI

Lateral

Bilateral neuropil regions just lateral to the PI

PI

Pars intercerebralis

PI-NSCs

Neurosecretory cells located in the pars intercerebralis

NSCs

Neurosecretory cells

Notes

Acknowledgements

This study was supported by a Grant-in-Aid for JSPS Research Fellows to HT (Grant numbers 13J04819 and 17J03649). All procedures performed in studies involving animal care and collection were in accordance with laws, ordinances, and the ethical standards of our institution and government.

Supplementary material

359_2018_1281_MOESM1_ESM.docx (114 kb)
Supplementary material 1 (DOCX 114 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Graduate School of ScienceOsaka City UniversityOsakaJapan
  2. 2.Research Fellow for Young ScientistsJapan Society for the Promotion of ScienceTokyoJapan
  3. 3.Graduate School of ScienceKyoto UniversityKyotoJapan
  4. 4.Faculty of ScienceUniversity of the RyukyusNishiharaJapan
  5. 5.Graduate School of ScienceOsaka UniversityToyonakaJapan

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