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Journal of Comparative Physiology A

, Volume 203, Issue 8, pp 635–647 | Cite as

Effects of juvenile hormone analogue (methoprene) and 20-hydroxyecdysone on reproduction in Polygonia c-aureum (Lepidoptera: Nymphalidae) in relation to adult diapause

  • Satoshi Hiroyoshi
  • Gadi V. P. Reddy
  • Jun Mitsuhashi
Original Paper

Abstract

We investigated the effects of juvenile hormone analogue (methoprene) and 20-hydroxyecdysone on female and male reproduction in a nymphalid butterfly, Polygonia c-aureum. This butterfly has a facultative adult diapause controlled by the corpora allata and brain. Methoprene seems to terminate reproductive diapause, although transplantation experiments indicate that the activity of the corpora allata does not affect male mating behavior Endo (Dev Growth Differ 15:1–10, 1973a), suggesting that the brain may be involved in diapause. We found that exposure to methoprene promoted the development of ovaries and of the male accessory glands and simplex. On the other hand, exposure to 20-hydroxyecdysone did not promote the development of female and male reproductive organs and eupyrene sperm movement from the testis to the duplex in the adult stage. Ecdysteroid titer in both sexes was consistently low in adults. These results suggest that imaginal diapause is largely regulated by juvenile hormone in this butterfly.

Keywords

Accessory gland Corpora allata Ecdysteroid Testis Simplex 

Abbreviations

CA

Corpus allatum

JH

Juvenile hormone

MAG

Male accessory gland

AGPs

Accessory gland products

JHA

Juvenile hormone analogue

PTTH

Prothoracicotropic hormone

LD

Long daylength

SD

Short daylength

RIA

Radioimmunoassay

20E

20-hydroxyecdysone

NPF

Neuropeptide F

Notes

Acknowledgements

We would like to thank Dr. N. Agui, Dr. T. Shimizu and Mr. M. Wani for their help. Thanks are also due to Dr. S. Moriya, Dr. K. Tateishi, and Mr. K. Takasino for sending the materials.

Compliance with ethical standards

Human and animal rights

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Ethical standards

We have not submitted our manuscript to other journal at present.

Conflict of interest

No competing interests declared.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Satoshi Hiroyoshi
    • 1
    • 2
  • Gadi V. P. Reddy
    • 3
  • Jun Mitsuhashi
    • 1
    • 4
  1. 1.Laboratory of Applied Entomology, Faculty of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.ItomanJapan
  3. 3.Western Triangle Ag Research CenterMontana State UniversityConradUSA
  4. 4.TokyoJapan

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