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Journal of Comparative Physiology A

, Volume 200, Issue 3, pp 183–195 | Cite as

Effect of GABAergic inhibition on odorant concentration coding in mushroom body intrinsic neurons of the honeybee

  • Anja Froese
  • Paul Szyszka
  • Randolf Menzel
Original Paper

Abstract

Kenyon cells, the intrinsic neurons of the insect mushroom body, have the intriguing property of responding in a sparse way to odorants. Sparse neuronal codes are often invariant to changes in stimulus intensity and duration, and sparse coding often depends on global inhibition. We tested if this is the case for honeybees’ Kenyon cells, too, and used in vivo Ca2+ imaging to record their responses to different odorant concentrations. Kenyon cells responded not only to the onset of odorant stimuli (ON responses), but also to their termination (OFF responses). Both, ON and OFF responses increased with increasing odorant concentration. ON responses were phasic and invariant to the duration of odorant stimuli, while OFF responses increased with increasing odorant duration. Pharmacological blocking of GABA receptors in the brain revealed that ionotropic GABAA and metabotropic GABAB receptors attenuate Kenyon cells’ ON responses without changing their OFF responses. Ionotropic GABAA receptors attenuated Kenyon cell ON responses more strongly than metabotropic GABAB receptors. However, the response dynamic, temporal resolution and paired-pulse depression did not depend on GABAA transmission. These data are discussed in the context of mechanisms leading to sparse coding in Kenyon cells.

Keywords

Kenyon cells Ca2+ imaging Olfactory coding GABA receptors OFF responses 

Abbreviations

GABA

γ-Aminobutyric acid

cKC

Clawed Kenyon cell

MB

Mushroom body

PCT

Protocerebral-calycal tract

BMI

Bicuculline methiodide

PTX

Picrotoxin

CGP

CGP54626

ROI

Region of interest

Notes

Acknowledgments

We are grateful to Drs. Silke Sachse, Melanie Haehnel and Nobuhiro Yamagata for their help and advice during the experiments. The work was supported by a grant of the Deutsche Forschungsgemeinschaft (Me 365/31-1).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Institut für NeurobiologieFreie Universität BerlinBerlinGermany
  2. 2.Department of Biology, NeurobiologyUniversity of KonstanzConstanceGermany

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