Journal of Comparative Physiology A

, Volume 199, Issue 8, pp 681–691 | Cite as

Social signals increase monoamine levels in the tegmentum of juvenile Mexican spadefoot toads (Spea multiplicata)

  • Verónica G. Rodriguez Moncalvo
  • Sabrina S. Burmeister
  • Karin S. Pfennig
Original Paper

Abstract

Monoamines are important neuromodulators that respond to social cues and that can, in turn, modify social responses. Yet we know very little about the ontogeny of monoaminergic systems and whether they contribute to the development of social behavior. Anurans are an excellent model for studying the development of social behavior because one of its primary components, phonotaxis, is expressed early in life. To examine the effect of social signals on monoamines early in ontogeny, we presented juvenile Mexican spadefoot toads (Spea multiplicata) with a male mating call or no sound and measured norepinephrine, epinephrine, dopamine, serotonin, and a serotonin metabolite, across the brain using high-pressure liquid chromatography. Our results demonstrate that adult-like monoaminergic systems are in place shortly after metamorphosis. Perhaps more interestingly, we found that mating calls increased the level of monoamines in the juvenile tegmentum, a midbrain region involved in sensory-motor integration and that contributes to brain arousal and attention. We saw no such increase in the auditory midbrain or in forebrain regions. We suggest that changes in monoamine levels in the juvenile tegmentum may reflect the effects of social signals on arousal state and could contribute to context-dependent modulation of social behavior.

Keywords

Monoamines Neuromodulator Anuran Acoustic communication HPLC 

Abbreviations

5-HIAA

5-Hydroxyindoleacetic acid

5-HT

5-Hydroxytryptamine (serotonin)

A-D di

Anterior-dorsal diencephalon

DA

Dopamine

DOPAC

3,4-Dihydroxyphenylacetic acid

E

Epinephrine

HVA

Homovanillic acid

ISO

Isoproterenol

MHPG

3-Methoxy-4-hydroxyphenylglycol

NE

Norepinephrine

P

Pallium

P-D di

Posterior-dorsal diencephalon

PoA

Preoptic area

P-V di

Posterior-ventral diencephalon

SC

Suprachiasmatic nucleus

SubP

Subpallium

Teg

Tegmentum

TS

Torus semicircularis

Notes

Acknowledgments

We thank Keith W. Sockman for use of his HPLC system and S. B. Southerland for his expertise in measuring monoamines. This work was supported by a New Innovator Award from the Office of the Director, National Institutes of Health (1 DP2 OD004436-01) to K. S. Pfennig. The University of North Carolina Institutional Animal Care and Use Committee approved all animal procedures.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Verónica G. Rodriguez Moncalvo
    • 1
    • 3
  • Sabrina S. Burmeister
    • 1
    • 2
  • Karin S. Pfennig
    • 1
  1. 1.Department of BiologyUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Curriculum in NeurobiologyUniversity of North Carolina at Chapel HillChapel HillUSA
  3. 3.Department of Biomedical SciencesUniversity of GuelphGuelphCanada

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