Journal of Comparative Physiology A

, Volume 198, Issue 6, pp 427–449 | Cite as

Adult neurogenesis in the brain of the Mozambique tilapia, Oreochromis mossambicus

  • Magda C. Teles
  • Ruxandra F. Sîrbulescu
  • Ursula M. Wellbrock
  • Rui F. Oliveira
  • Günther K. H. Zupanc
Original Paper

Abstract

Although the generation of new neurons in the adult nervous system (‘adult neurogenesis’) has been studied intensively in recent years, little is known about this phenomenon in non-mammalian vertebrates. Here, we examined the generation, migration, and differentiation of new neurons and glial cells in the Mozambique tilapia (Oreochromis mossambicus), a representative of one of the largest vertebrate taxonomic orders, the perciform fish. The vast majority of new cells in the brain are born in specific proliferation zones of the olfactory bulb; the dorsal and ventral telencephalon; the periventricular nucleus of the posterior tuberculum, optic tectum, and nucleus recessi lateralis of the diencephalon; and the valvula cerebelli, corpus cerebelli, and lobus caudalis of the cerebellum. As shown in the olfactory bulb and the lateral part of the valvula cerebelli, some of the young cells migrate from their site of origin to specific target areas. Labeling of mitotic cells with the thymidine analog 5-bromo-2′-deoxyuridine, combined with immunostaining against the neuron-specific marker protein Hu or against the astroglial marker glial fibrillary acidic protein demonstrated differentiation of the adult-born cells into both neurons and glia. Taken together, the present investigation supports the hypothesis that adult neurogenesis is an evolutionarily conserved vertebrate trait.

Keywords

Teleosts Perciformes Olfactory bulb Hippocampus Cerebellum 

Abbreviations

A

Anterior thalamic nucleus

AC

Anterior commissure

BO

Olfactory bulb

BOgl

Glomerular layer of the olfactory bulb

BOgra

Granular layer of the olfactory bulb

bv

Blood vessel

CC

Crista cerebellaris

CCe

Corpus cerebelli

CCegra

Granular layer of corpus cerebelli

CCemol

Molecular layer of corpus cerebelli

CE

Central nucleus of the inferior lobe

ch

Horizontal commissure

CM

Corpus mamillare

CP

Central posterior thalamic nucleus

Cven

Ventral rhombencephalic commissure

D

Dorsal telencephalon

DA

Anterior part of the dorsal telencephalon

DC

Dorsocentral telencephalon

DCa

Anterior subdivision of the dorsal central telencephalon

DCad

Dorsal part of the anterior subdivision of the dorsocentral telencephalon

DCm

Medial subdivision of the dorsocentral telencephalon

DD

Dorsal division of the dorsal telencephalon

DFl

Nucleus diffusus lateralis of the inferior lobe

DFld

Dorsal subdivision of nucleus diffusus lateralis of the inferior lobe

DFlv

Ventral subdivision of nucleus diffusus lateralis of the inferior lobe

DFm

Nucleus diffusus medialis of the inferior lobe

DL

Dorsolateral telencephalon

DLa

Anterior subdivision of the dorsolateral telencephalon

DLd

Dorsal subdivision of the dosolateral telencephalon

DLp

Posterior subdivision of the dorsolateral telencephalon

DLv

Ventral subdivision of the dorsolateral telencephalon

DM

Dorsomedial telencephalon

DMa

Anterior subdivision of the dorsomedial telencephalon

DMdd

Dorsal part of the dorsal subdivision of the dorsomedial telencephalon

DMdv

Ventral part of the dorsal subdivision of the dorsomedial telencephalon

DMv

Ventral subdivision of the dorsomedial telencephalon

DMvd

Dorsal part of the ventral subdivision of the dorsomedial telencephalon

DMvv

Ventral part of the ventral subdivision of the dorsomedial telencephalon

DP

Dorsal posterior thalamic nucleus

Dp

Posterior part of dorsal telencephalon

EG

Eminentia granularis

G

Glomerular nucleus

GC

Central gray

H

Habenula

Hv

Ventral zone of the periventricular hypothalamus

IL

Inferior lobe of the hypothalamus

ILdl

Dorsolateral subdivision of the inferior lobe of the hypothalamus

ILvm

Ventromedial subdivision of the inferior lobe of the hypothalamus

IPn

Interpeduncular nucleus

LCe

Lobus caudalis

LCegra

Granular layer of the lobus caudalis

LH

Lateral hypothalamic nucleus

LVII

Lobus facialis

LX

Lobus vagi

MLF

Medial longitudinal fascicle

mol

Molecular layer

nIII

Nucleus oculomotorius

nRL

Nucleus recessi lateralis

nRLl

Lateral part of the nucleus recessi lateralis

nRLm

Medial part of the nucleus recessi lateralis

nRP

Nucleus recessi posterioris

nVd

Descending trigeminal nucleus

nX

Nucleus X (unidentified nucleus located between tOv and DFld)

P

Preoptic region

PGm

Medial part of the preglomerular nucleus

PP

Periventricular preoptic nucleus

PPa

Anterior part of the periventricular preoptic nucleus

PPm

Medial part of the periventricular preoptic nucleus

PPp

Posterior part of the periventricular preoptic nucleus

RL

Recessus lateralis

RP

Recessus posterioris

RV

Rhombencephalic ventricle

Sc

Suprachiasmatic nucleus

TA

Nucleus anterior tuberis

TeO

Optic tectum

TGN

Tertiary gustatory nucleus

TGNl

Lateral part of the tertiary gustatory nucleus

TGNm

Medial part of the tertiary gustatory nucleus

TL

Torus longitudinalis

TLA

Torus lateralis

TLAi

Inferior subdivision of the torus lateralis

tO

Optic tract

tOd

Tractus opticus dorsalis

tolfm

Medial part of the olfactory tract

tOv

Tractus opticus ventralis

TP

Nucleus of the posterior tuberculum

TPP

Periventricular nucleus of the posterior tuberculum

TS

Torus semicircularis

tTB

Tractus tecto-bulbaris

V

Ventral telencephalon

v

Ventricle

Val

Lateral part of valvula cerebelli

Valgra

Granular layer of the lateral part of valvula cerebelli

Valmol

Molecular layer of the lateral part of the valvula cerebelli

Vam

Medial part of valvula cerebelli

Vamgra

Granular layer of the medial part of the valvula cerebelli

Vammol

Molecular layer of the medial part of the valvula cerebelli

Vd

Dorsal part of the ventral telencephalon

VLR

Ventrolateral rhombencephalon

VM

Ventromedial thalamic nucleus

Vs

Supracommissural part of the ventral telencephalon

VVm

Medial part of the ventral subdivision of the ventral telencephalon

Notes

Acknowledgments

We thank Iulian Ilieş for helpful comments on a previous version of the manuscript. This study was funded by Tönjes-Vagt-Stiftung and Deutscher Akademischer Austauschdienst (to G.K.H.Z.); Gabinete de Relações Internacionais da Ciência e do Ensino Superior German-Portuguese exchange grant and Fundação Portuguesa para a Ciência e a Tecnologia research grant # PTDC/PSI/71811/2006 (to R.F.O.); and Ph.D.fellowship # SFRH/BD/44848/2008 (to M.C.T.). All experiments were carried out in accordance with the relevant law, the Deutsches Tierschutzgesetz of 1998.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Magda C. Teles
    • 1
    • 2
    • 3
  • Ruxandra F. Sîrbulescu
    • 3
    • 4
  • Ursula M. Wellbrock
    • 3
  • Rui F. Oliveira
    • 1
    • 2
  • Günther K. H. Zupanc
    • 3
    • 4
  1. 1.Unidade de Investigação em Eco-EtologiaInstituto Superior de Psicologia AplicadaLisbonPortugal
  2. 2.Champalimaud Neuroscience ProgrammeInstituto Gulbenkian de CiênciaOeirasPortugal
  3. 3.School of Engineering and ScienceJacobs University BremenBremenGermany
  4. 4.Department of BiologyNortheastern UniversityBostonUSA

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