Journal of Comparative Physiology A

, Volume 196, Issue 2, pp 147–154 | Cite as

Effects of capture stress on free-ranging, reproductively active male Weddell seals

  • Robert Geoffrey Harcourt
  • Emma Turner
  • Ailsa Hall
  • Joseph R. Waas
  • Mark Hindell
Original Paper


Physiological stress responses to capture may be an indicator of welfare challenges induced by animal handling. Simultaneously, blood chemistry changes induced by stress responses may confound experimental design by interacting with the biological parameters being measured. Cortisol elevation is a common indicator of stress responses in mammals and reproductive condition can profoundly influence endocrine response. We measured changes in blood cortisol and testosterone induced by handling reproductively active male Weddell seals (Leptonychotes weddellii) early and late in the breeding season. Weddell seals have the highest resting cortisol levels of all mammals yet showed a clear, prolonged elevation in cortisol in response to capture. Responses were similar when first caught and when caught a second time, later in the breeding season. Baseline testosterone levels declined over the breeding season but were not altered by capture. Administering a light dose of diazepam significantly ameliorated the cortisol response of handled animals without affecting testosterone levels. This may be an effective way of reducing acute capture stress responses. Male breeding success in years males were handled was no different to the years they were not, despite the acute capture response, suggesting no long-term impact of handling on male reproductive output.


Marine mammals Leptonychotes weddellii Handling stress Cortisol Antarctica 



We would like to thank the staff of Scott Base and Antarctica New Zealand who provided excellent field support for 4 years of this study. We thank Dudley Bell, Tony Dorr, and Sarah Winter for field assistance. Randy Davis, Terrie Williams, Tom Gelatt and Mike Cameron provided invaluable support at various times. Two anonymous reviewers provided comments which improved the manuscript. The study was supported by the Seaworld Research and Rescue Foundation, the Australian Research Council, the Graduate School of the Environment, Macquarie University, the Department of Biological Sciences, University of Waikato and the Antarctic Scientific Advisory Committee. Permission to conduct the study was obtained from the Environmental Assessment and Review Panel of Antarctica New Zealand, the Department of Conservation, New Zealand and the Animal Ethics Committee of the University of Waikato.


  1. Anderson SS, Fedak MA (1985) Grey seal males; energetic and behavioural links between size and sexual success. Anim Behav 33:829–838CrossRefGoogle Scholar
  2. Anderson SS, Fedak MA (1987) Grey seal, Halichoerus grypus, energetics: females invest more in male offspring. J Zool (Lond) 211:667–679CrossRefGoogle Scholar
  3. Arnbom T, Fedak MA, Boyd IL (1997) Factors affecting maternal expenditure in southern elephant seals during lactation. Ecology 78:471–483Google Scholar
  4. Barrell GK, Montgomery GW (1989) Absence of circadian patterns of secretion of melatonin or cortisol in Weddell seals under continuous natural daylight. J Endocr 122:445–449CrossRefPubMedGoogle Scholar
  5. Bartsh SS, Johnston SD, Siniff DB (1992) Territorial behavior and breeding frequency of male Weddell seals (Leptonychotes weddelli) in relation to age, size, and concentrations of serum testosterone and cortisol. Can J Zool 70:680–692CrossRefGoogle Scholar
  6. Boyd IL, Duck CD (1991) Mass changes and metabolism in territorial male Antarctic fur seals (Arctocephalus gazella). Physiol Zool 64:375–392Google Scholar
  7. Cameron MF, Siniff DB (2004) Age-specific survival, abundance, and immigration of a Weddell seal (Leptonychotes weddellii) population in McMurdo Sound, Antarctica. Can J Zool 82:601–615CrossRefGoogle Scholar
  8. Davis AK, Maney DL, Maerz JC (2008) The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Funct Ecol 26:760–772CrossRefGoogle Scholar
  9. De Villiers MS, Van Jaarsveld AS, Meltzer DGA, Richardson PRK (1997) Social dynamics and the cortisol response to immobilization stress of the African wild dog, Lycaon pictus. Horm Behav 31:3–14CrossRefPubMedGoogle Scholar
  10. Engelhard GH, van den Hoff J, Broekman M, Baarspul ANJ, Field I, Slip DJ, Burton HR, Reijnders PJH (2001) Mass of weaned elephant seal pups in areas of low and high human presence. Polar Biol 24:244–251CrossRefGoogle Scholar
  11. Engelhard GH, Brasseur SMJM, Hall AJ, Burton H, Reijinders PJH (2002a) Adrenocortical responsiveness in southern elephant seal mothers and pups during lactation and the effect of scientific handling. J Comp Physiol B Biochem Syst Environ Phys 172:315–328CrossRefGoogle Scholar
  12. Engelhard GH, Hall AJ, Brasseur SMJM, Reijinders PJH (2002b) Blood chemistry in southern elephant seal mothers and pups during lactation reveals no effect of handling. Comp Biochem Physiol Part A Mol Int Physiol 133A:367–378CrossRefGoogle Scholar
  13. Erickson AW, Bester MN (1993) Immobilization and capture. In: Laws RM (ed) Antarctic seals: research methods and techniques. Cambridge University Press, Cambridge, pp 46–88Google Scholar
  14. Fedak MA, Anderson SS (1982) The energetics of lactation: accurate measurements from a large wild mammal, the grey seal (Halichoerus grypus). J Zool (Lond) 200:298–300CrossRefGoogle Scholar
  15. Fraser D, Ritchie JSD, Fraser AH (1975) The term stress in a veterinary context. Brit Vet J 131:653–662Google Scholar
  16. Gardiner KJ, Hall AJ (1997) Diel and annual variation in plasma cortisol concentrations among wild and captive harbor seals (Phoca vitulina). Can J Zool 75:1773–1780CrossRefGoogle Scholar
  17. Harcourt RG, Hindell MA, Waas JR (1998) Under-ice movements and territory use in free-ranging Weddell seals during the breeding season. N Z Nat Sci 23:72–73Google Scholar
  18. Harcourt RG, Hindell MA, Bell DG, Waas JR (2000) Three-dimensional dive profiles of free-ranging Weddell seals. Polar Biol 23:479–487CrossRefGoogle Scholar
  19. Harcourt RG, Kingston JJ, Cameron M, Waas JR, Hindell MA (2007) Paternity analysis shows experience, not age, enhances mating success in an aquatically mating pinniped the Weddell seal (Leptonychotes weddellii). Behav Ecol Sociobiol 61:643–652CrossRefGoogle Scholar
  20. Harcourt RG, Kingston JJ, Waas JR, Hindell MA (2008) Foraging while breeding: alternative mating strategies by male Weddell seals? Aquat Cons 17:S68–S78CrossRefGoogle Scholar
  21. Hewison AJM, Andersen R, Gaillard JM, Linnell JDC, Delorme D (1999) Contradictory findings in studies of sex ratio variation in roe deer (Capreolus capreolus). Behav Ecol Sociobiol 45:339–348CrossRefGoogle Scholar
  22. Hill S (1987) Reproductive ecology of Weddell seals (Leptnychotes weddellii) in McMurdo Sound, Antarctica. PhD thesis, University of Minnesota, MinnesotaGoogle Scholar
  23. Hindell MA, Harcourt RG, Waas JR, Thompson D (2002) Fine-scale, three dimensional spatial use of diving lactating female Weddell seals, Leptonychotes weddellii. Mar Ecol Prog Ser 272:275–284CrossRefGoogle Scholar
  24. Kaufman GW, Siniff DB, Reichle R (1975) Colony behavior of Weddell seals (Leptonychotes weddellii), at Hutton Cliffs, Antarctica. Rapports et Proces-verbaux des Reunions 169:228–246Google Scholar
  25. Kelly BP (1996) Live capture of ringed seals in ice-covered waters. J Wildl Manag 60:678–684CrossRefGoogle Scholar
  26. Le Maho Y, Karmann H, Briot D, Handrich Y, Robin J, Mioskowski E, Cherel Y, Farni J (1992) Stress in birds due to routine handling and a technique to avoid it. Am J Physiol 263:R775–R781PubMedGoogle Scholar
  27. Lidgard DC, Boness DJ, Bowen WD (2001) A novel mobile approach to investigating mating tactics in male grey seals (Halichoerus grypus). J Zool 255:313–320CrossRefGoogle Scholar
  28. Liggins GC, France JT, Knox BS, Zapol WM (1979) High corticosteroid levels in plasma of adult and fetal Weddell seals Leptonychotes weddelli. Acta Endocr 90:718–726PubMedGoogle Scholar
  29. Liggins GC, France JT, Schneider RC, Knox BS, Zapol WM (1993) Concentrations, metabolic clearance rates, production rates and plasma binding of cortisol in Antarctic phocid seals. Acta Endocr 129:356–359PubMedGoogle Scholar
  30. McMahon C, van den Hoff J, Burton H (2005) Handling intensity and the short- and long-term survival of elephant seals: addressing and quantifying research effects on wild animals. Ambio 34:426–429PubMedGoogle Scholar
  31. McMahon CR, Field IC, Bradshaw CJA, White GC, Hindell MA (2008) Tracking and data-logging devices attached to elephant seals do not affect individual mass gain or survival. J Exp Mar Biol Ecol 360:71–77CrossRefGoogle Scholar
  32. Morton DJ, Anderson E, Foggin CM, Kock MD, Tiran EP (1995) Plasma cortisol as an indicator of stress due to capture and translocation in wildlife species. Vet Rec 136:60–63PubMedGoogle Scholar
  33. Ninnes CE, Waas JR, Ling N, Nakagawa S, Banks JC, Bell DG, Bright A, Carey PW, Chandler J, Hudson QJ, Ingram JR, Lyall K, Morgan DK, Stevens MI, Wallace J, Möstl E (2009) Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae. J Comp Physiol B. doi: 10.1007/s00360-009-0390-0
  34. O’Reilly KM, Wingfield JC (2001) Ecological factors underlying the adrenocortical response to capture stress in arctic-breeding shorebirds. Gen Comp Endocr 124:1–11CrossRefPubMedGoogle Scholar
  35. Omsjoe EH, Stien A, Irvine J, Albon SD, Dahl E, Thoresen SI, Rustad E, Ropstad E (2009) Evaluating capture stress and its effects on reproductive success in Svalbard reindeer. Can J Zool 87:73–85CrossRefGoogle Scholar
  36. Ortiz RM, Worthy GAJ (2000) Effects of capture on adrenal steroid and vasopressin concentrations in free-ranging bottlenose dolphins (Tursiops truncatus). Comp Biochem Physiol Part A 125:317–324CrossRefGoogle Scholar
  37. Pomeroy P, Fedak MA, Rothery P, Anderson S (1999) Consequences of maternal size for reproductive expenditure and pupping success of grey seals at North Rona, Scotland. J Anim Ecol 68:235–253CrossRefGoogle Scholar
  38. Powell LA, Condroy MJ, Hines JE, Nichols JD, Krementz DG (2000) Simultaneous use of mark-recapture and radiotelemetry to estimate survival, movement and capture rates. J Wildl Manag 64:302–313CrossRefGoogle Scholar
  39. Ramsey MA, Stirling I (1988) Reproductive biology and ecology of female polar bears (Ursus maritimus). J Zool (Lond) 214:601–634CrossRefGoogle Scholar
  40. Sapolsky RM (1985) Stress-induced suppression of testicular function in the wild baboon Papio anubis role of glucocorticoids. Endocrinology 116:2273–2278CrossRefPubMedGoogle Scholar
  41. Siniff DB, Demaster DP, Hofman RJ, Eberhardt LL (1977) An analysis of the dynamics of a Weddell seal population. Ecol Monographs 47:319–335CrossRefGoogle Scholar
  42. Stirling I (1966) A technique for handling live seals. J Mammal 47:543–544CrossRefGoogle Scholar
  43. Stirling I (1969) Ecology of the Weddell seal in McMurdo Sound, Antarctica. Ecology 50:574–585CrossRefGoogle Scholar
  44. Testa JW, Siniff DB (1987) Population dynamics of Weddell seals (Leptonychotes weddelli) in McMurdo Sound Antarctica. Ecol Monogr 57:149–165CrossRefGoogle Scholar
  45. Thomson CA, Geraci JR (1986) Cortisol aldosterone and leukocytes in the stress response of bottlenose dolphins Tursiops truncatus. Can J Fish Aquat Sci 43:1010–1016CrossRefGoogle Scholar
  46. van Polanen Petel TD, Giese MA, Wotherspoon S, Hindell MA (2007) The behavioural response of lactating Weddell seals (Leptonychotes weddellii) to over-snow vehicles: a case study. Can J Zool 85:488–496CrossRefGoogle Scholar
  47. Vleck CM, Vertalino N, Vleck D, Bucher TL (2000) Stress, corticosterone, and heterophil to lymphocyte ratios in free-living Adélie penguins. Condor 102:392–400CrossRefGoogle Scholar
  48. Wheatley KE, Bradshaw CJA, Davis LS, Harcourt RG, Hindell MA (2006) Influence of maternal mass and condition on energy transfer in Weddell seals. J Anim Ecol 75:724–733CrossRefPubMedGoogle Scholar
  49. Wheatley KE, Nichols PD, Hindell MA, Harcourt RG, Bradshaw CJA (2007) Temporal variation in the vertical stratification of blubber fatty acids alters diet predictions for lactating Weddell seals. J Exp Mar Biol Ecol 352:103–113CrossRefGoogle Scholar
  50. Wheatley KE, Bradshaw CJA, Harcourt RG, Hindell MA (2008a) Feast or famine: evidence for mixed capital-income breeding strategies in the Weddell seal. Oecologia 155:11–20CrossRefPubMedGoogle Scholar
  51. Wheatley KE, Nichols PD, Hindell MA, Harcourt RG, Bradshaw CJA (2008b) Differential mobilization of blubber fatty acids in lactating Weddell seals: Evidence for selective use. Physiol Biochem Zool 81:651–662CrossRefPubMedGoogle Scholar
  52. Wilson RP, McMahon CR (2006) Measuring devices on wild animals: what constitutes acceptable practice? Front Ecol Environ 4:147–154CrossRefGoogle Scholar
  53. Wingfield JC (1994) Modulation of the adrenocortical response to stress in birds. In: Davey KG, Peter RE, Tobe SS (eds) Perspectives in comparative endocrinology. National Research Council of Canada, Ottawa, pp 520–528Google Scholar
  54. Wingfield JC, Sapolsky RM (2003) Reproduction and resistance to stress: when and how. J Neuroendocr 15:711–724Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Robert Geoffrey Harcourt
    • 1
  • Emma Turner
    • 1
  • Ailsa Hall
    • 2
  • Joseph R. Waas
    • 3
  • Mark Hindell
    • 4
  1. 1.Marine Mammal Research Group, Graduate School of the EnvironmentMacquarie UniversitySydneyAustralia
  2. 2.Sea Mammal Research Unit, Scottish Oceans InstituteSt Andrews UniversitySt Andrews, FifeUK
  3. 3.Department of Biological SciencesUniversity of WaikatoHamiltonNew Zealand
  4. 4.Antarctic Wildlife Research Unit, Department of ZoologyUniversity of TasmaniaHobartAustralia

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