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Journal of Comparative Physiology A

, Volume 194, Issue 9, pp 829–839 | Cite as

Changes in food intake and glucosensing function of hypothalamus and hindbrain in rainbow trout subjected to hyperglycemic or hypoglycemic conditions

  • Sergio Polakof
  • Jesús M. Míguez
  • José L. SoengasEmail author
Original Paper

Abstract

To evaluate the possible role of glucose in the control of food intake (FI) in fish and the involvement of glucosensing system in that role, we have subjected rainbow trout (via intraperitoneal injections) to control, hyperglycemic (500 mg kg−1 glucose body mass) or hypoglycemic (4 mg kg−1 bovine insulin) conditions for 10 days. The experimental design was appropriate since hypoglycemia and hyperglycemia were observed the first 5 days after treatment and changes observed in metabolic parameters in liver were similar to those of fish literature. Hyperglycemic conditions elicited small changes in FI accompanied by increased glucose and glycogen levels, glucokinase (GK) activity and glycolytic potential in hypothalamus and hindbrain. In contrast, hypoglycemic conditions elicited a marked increase in FI accompanied by decreased glucose and glycogen levels and GK activity in the same brain regions whereas both regions displayed different responses in glycolytic potential. These results allow us to hypothesize that, despite the relative intolerance to glucose of carnivorous fish, changes in plasma glucose levels in rainbow trout detected by glucosensing areas in brain regions (hypothalamus and hindbrain) are integrated in those or near areas eliciting a response in FI, which was more important under hypoglycemic than under hyperglycemic conditions.

Keywords

Food intake Rainbow trout Glucosensing Hypothalamus Hindbrain 

Abbreviations

BB

Brockmann bodies

CART

Cocaine- and amphetamine-regulated transcript

CCK

Cholecystokinin

HK

Low Km hexokinase (EC. 2.7.1.11.)

FBPase

Fructose 1,6-bisphosphatase (EC. 3.1.3.11.)

G6Pase

Glucose 6-phosphatase (EC. 3.1.3.9.)

GK

Hexokinase IV or glucokinase (EC. 2.7.1.2.)

GLUT-2

Glucose facilitative transporter type 2

GSase

Glycogen synthase (EC. 2.4.1.11.)

NPY

Neuropeptide Y

PK

Pyruvate kinase (EC. 2.7.1.40.)

Notes

Acknowledgments

This study was supported by research grants from Ministerio de Educación y Ciencia and European Fund for Regional Development (AGL2007-65744-C03-01/ACU), and Universidade de Vigo (Contrato-Programa para grupos de investigación consolidados). Sergio Polakof was recipient of a predoctoral fellowship from the Xunta de Galicia (Program Maria Barbeito).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sergio Polakof
    • 1
  • Jesús M. Míguez
    • 1
  • José L. Soengas
    • 1
    Email author
  1. 1.Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de BioloxíaUniversidade de VigoVigoSpain

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