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Journal of Comparative Physiology A

, Volume 190, Issue 4, pp 301–309 | Cite as

Neuromodulation for behavior in the locust frontal ganglion

  • Y. Zilberstein
  • E. Fuchs
  • L. Hershtik
  • A. Ayali
Original Paper

Abstract

Neuromodulators orchestrate complex behavioral routines by their multiple and combined effects on the nervous system. In the desert locust, Schistocerca gregaria, frontal ganglion neurons innervate foregut dilator muscles and play a key role in the control of foregut motor patterns. To further investigate the role of the frontal ganglion in locust behavior, we currently focus on the frontal ganglion central pattern generator as a target for neuromodulation. Application of octopamine, a well-studied insect neuromodulator, generated reversible disruption of frontal ganglion rhythmic activity. The threshold for the modulatory effects of octopamine was 10−6 mol l−1, and 10−4 mol l−1 always abolished the ongoing rhythm. In contrast to this straightforward modulation, allatostatin, previously reported to be a myoinhibitor of insect gut muscles, showed complex, tri-modal, dose-dependent effects on frontal ganglion rhythmic pattern. Using a novel cross-correlation analysis technique, we show that different allatostatin concentrations have very different effects not only on cycle period but also on temporal characteristics of the rhythmic bursts of action potentials. Allatostatin also altered the frontal ganglion rhythm in vivo. The analysis technique we introduce may be instrumental in the study of not fully characterized neural circuits and their modulation. The physiological significance of our results and the role of the modulators in locust behavior are discussed.

Keywords

Allatostatin Cross-correlation Neuromodulation Octopamine Schistocerca gregaria 

Abbreviation

CPG

central pattern generator

FG

frontal ganglion

JH

juvenile hormone

STNS

stomatogastric nervous system

Notes

Acknowledgements

Support for this work came from the Israel Science Foundation 410/02 and the USA-Israel Binational Science Foundation (BSF) 9800456. Y.Z. carried out most of the experimental work, E.F. contributed to the data analysis, L.H. participated in early stages of the work, A.A. conceived the experiments and carried out part of them.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Y. Zilberstein
    • 1
  • E. Fuchs
    • 1
  • L. Hershtik
    • 1
  • A. Ayali
    • 1
  1. 1.Department of Zoology, Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael

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