Experiments in Fluids

, Volume 44, Issue 6, pp 851–863 | Cite as

Planar laser induced fluorescence in aqueous flows

Review Article


Planar laser-induced fluorescence (PLIF) is a non-intrusive technique for measuring scalar concentrations in fluid flows. A fluorescent dye is used as a scalar proxy, and local fluorescence caused by excitation from a thin laser sheet can be related to dye concentration. This review covers quantitative PLIF in aqueous flows, with discussions of fluorescence theory, experimental methods and equipment, image processing and calibration, and applications of the technique.


  1. Ai JJ, Law AWK, Yu SCM (2006) On boussinesq and non-boussinesq starting forced plumes. J Fluid Mech 558:357–386MATHGoogle Scholar
  2. Arcoumanis C, McGuirk JJ, Palma J (1990) On the use of fluorescent dyes for concentration measurements in water flows. Exp Fluids 10(2–3):177–180Google Scholar
  3. Arratia PE, Muzzio FJ (2004) Planar laser-induced fluorescence method for analysis of mixing in laminar flows. Ind Eng Chem Res 43(20):6557–6568Google Scholar
  4. Atsavapranee P, Gharib M (1997) Structures in stratified plane mixing layers and the effects of cross-shear. J Fluid Mech 342:53–86MathSciNetGoogle Scholar
  5. Axelrod D, Koppel DE, Schlessinger J, Elson E, Webb WW (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys J 16(9):1055–1069Google Scholar
  6. Barrett TK, Van Atta CW (1991) Experiments on the inhibition of mixing in stably stratified decaying turbulence using laser doppler anemometry and laser-induced fluorescence. Phys Fluids A Fluid Dyn 3(5):1321–1332Google Scholar
  7. Batchelor G (1959) Small-scale variation of convected quantities like temperature in turbulent fluid. J Fluid Mech 5:113–133MATHMathSciNetGoogle Scholar
  8. Bhat GS, Narasimha R (1996) A volumetrically heated jet: large-eddy structure and entrainment characteristics. J Fluid Mech 325:303–330Google Scholar
  9. Borg A, Bolinder J, Fuchs L (2001) Simultaneous velocity and concentration measurements in the near field of a turbulent low-pressure jet by digital particle image velocimetry-planar laser-induced fluorescence. Expe Fluids 31(2):140–152Google Scholar
  10. Bruchhausen M, Guillard F, Lemoine F (2005) Instantaneous measurement of two-dimensional temperature distributions by means of two-color planar laser induced fluorescence (PLIF). Exp Fluids 38:123–131Google Scholar
  11. Campbell JE, Coppom RW, Guilkey JE, Klewicki JC, McMurtry PA (2004) Time resolved concentration measurements in an axial flow mixer. J Fluids Eng Trans ASME 126(6):981–989Google Scholar
  12. Catrakis HJ, Dimotakis PE (1996) Mixing in turbulent jets: scalar measures and isosurface geometry. J Fluid Mech 317:369–406Google Scholar
  13. Cetegen BM, Mohamad N (1993) Experiments on liquid-mixing and reaction in a vortex. J Fluid Mech 249:391–414Google Scholar
  14. Chang KA, Cowen EA (2002) Turbulent prandtl number in neutrally buoyant turbulent round jet. J Eng Mech ASCE 128(10):1082–1087Google Scholar
  15. Chen DY, Chen CQ, Tang FE, Stansby P, Li M (2007) Boundary layer structure of oscillatory open-channel shallow flows over smooth and rough beds. Exp Fluids 42(5):719–736Google Scholar
  16. Coolen MCJ, Kieft RN, Rindt CCM, van Steenhoven AA (1999) Application of 2-D LIF temperature measurements in water using a Nd:YAG laser. Exp Fluids 27(5):420–426Google Scholar
  17. Coppeta J, Rogers C (1998) Dual emission laser induced fluorescence for direct planar scalar behavior measurements. Exp Fluids 25(1):1–15Google Scholar
  18. Cowen EA, Chang KA, Liao Q (2001) A single-camera coupled PTV-LIF technique. Exp Fluids 31(1):63–73Google Scholar
  19. Crimaldi JP (1997) The effect of photobleaching and velocity fluctuations on single-point LIF measurements. Exp Fluids 23(4):325–330Google Scholar
  20. Crimaldi JP, Koseff JR (2001) High-resolution measurements of the spatial and temporal scalar structure of a turbulent plume. Exp Fluids 31(1):90–102Google Scholar
  21. Crimaldi J, Koehl M, Koseff J (2002a) Effects of the resolution and kinematics of olfactory appendages on the interception of chemical signals in a turbulent odor plume. Environ Fluid Mech 2:35–63Google Scholar
  22. Crimaldi JP, Wiley MB, Koseff JR (2002b) The relationship between mean and instantaneous structure in turbulent passive scalar plumes. J Turbul 3 (014):1–24MathSciNetGoogle Scholar
  23. Dahm WJA, Dimotakis PE (1987) Measurements of entrainment and mixing in turbulent jets. AIAA J 25(9):1216–1223CrossRefGoogle Scholar
  24. Dahm WJA, Dimotakis PE (1990) Mixing at large schmidt number in the self-similar far field of turbulent jets. J Fluid Mech 217:299–330Google Scholar
  25. Dahm WJA, Southerland KB, Buch KA (1991) Direct, high-resolution, 4-dimensional measurements of the fine scale structure of Sc > 1 molecular mixing in turbulent flows. Phys Fluids A Fluid Dyn 3(5):1115–1127Google Scholar
  26. Dahm WJA, Su LK, Southerland KB (1992) A scalar imaging velocimetry technique for fully resolved 4-dimensional vector velocity-field measurements in turbulent flows. Phys Fluids A Fluid Dyn 4(10):2191–2206Google Scholar
  27. Davidson MJ, Pun KL (1999) Weakly advected jets in cross-flow. J Hydraul Eng ASCE 125(1):47–58Google Scholar
  28. Daviero GJ, Roberts PJW, Maile K (2001) Refractive index matching in large-scale stratified experiments. Exp Fluids 31(2):119–126Google Scholar
  29. Delo CJ, Kelso RM, Smits AJ (2004) Three-dimensional structure of a low-reynolds-number turbulent boundary layer. J Fluid Mech 512:47–83MATHGoogle Scholar
  30. Deusch S, Dracos T (2001) Time resolved 3D passive scalar concentration-field imaging by laser induced fluorescence (LIF) in moving liquids. Meas Sci Technol 12(2):188–200Google Scholar
  31. Dewey C (1976) Qualitative and quantitative flow field visualization utilizing laser-induced fluorescence. In: Proceedings of the AGARD conference of non-intrusive instrumentation in fluid flow research, AGARD-CP-193Google Scholar
  32. Diez F, Bernal LP, Faeth GM (2005) PLIF and PIV measurements of the self-preserving structure of steady round buoyant turbulent plumes in crossflow. Int J Heat Fluid Flow 26:873–882Google Scholar
  33. Dimotakis PE, Miakelye RC, Papantoniou DA (1983) Structure and dynamics of round turbulent jets. Phys Fluids 26(11):3185–3192Google Scholar
  34. Ferrier AJ, Funk DR, Roberts PJW (1993) Application of optical techniques to the study of plumes in stratified fluids. Dyn Atmos Oceans 20(1–2):155–183Google Scholar
  35. Guilkey JE, Gee KR, McMurtry PA, Klewicki JC (1996) Use of caged fluorescent dyes for the study of turbulent passive scalar mixing. Exp Fluids 21(4):237–242Google Scholar
  36. Guillard F, Fritzon R, Revstedt J, Tragardh C, Alden M, Fuchs L (1998) Mixing in a confined turbulent impinging jet using planar laser-induced fluorescence. Exp Fluids 25(2):143–150Google Scholar
  37. Hannoun IA, List EJ (1988) Turbulent mixing at a shear-free density interface. J Fluid Mech 189:211–234Google Scholar
  38. Hansen L, Guilkey JE, McMurtry PA, Klewicki JC (2000) The use of photoactivatable fluorophores in the study of turbulent pipe mixing: effects of inlet geometry. Meas Sci Technol 11(9):1235–1250Google Scholar
  39. Hishida K, Sakakibara J (2000) Combined planar laser-induced fluorescence-particle image velocimetry technique for velocity and temperature fields. Exp Fluids 29:S129–S140Google Scholar
  40. Horner-Devine AR (2006) Velocity, density and transport measurements in rotating, stratified flows. Exp Fluids 41(4):559–571Google Scholar
  41. Houcine I, Vivier H, Plasari E, David R, Villermaux J (1996) Planar laser induced fluorescence technique for measurements of concentration fields in continuous stirred tank reactors. Exp Fluids 22(2):95–102Google Scholar
  42. Johari H (1992) Mixing in thermals with and without buoyancy reversal. J Atmos Sci 49(16):1412–1426Google Scholar
  43. Karasso PS, Mungal MG (1996) Scalar mixing and reaction in plane liquid shear layers. J Fluid Mech 323:23–63Google Scholar
  44. Karasso PS, Mungal MG (1997) PLIF measurements in aqueous flows using the Nd:YAG laser. Exp Fluids 23(5):382–387Google Scholar
  45. Koehl MAR, Koseff JR, Crimaldi JP, McCay MG, Cooper T, Wiley MB, Moore PA (2001) Lobster sniffing: antennule design and hydrodynamic filtering of information in an odor plume. Science 294:1948–1951Google Scholar
  46. Koga DJ, Abrahamson SD, Eaton JK (1987) Development of a portable laser sheet. Exp Fluids 5(3):215–216Google Scholar
  47. Komori S, Nagata K, Kanzaki T, Murakami Y (1993) Measurements of mass flux in a turbulent liquid flow with a chemical-reaction. AICHE J 39(10):1611–1620Google Scholar
  48. Koochesfahani MM, Dimotakis PE (1985) Laser-induced fluorescence measurements of mixed fluid concentration in a liquid plane shear-layer. AIAA J 23(11):1700–1707Google Scholar
  49. Koochesfahani MM, Dimotakis PE (1986) Mixing and chemical-reactions in a turbulent liquid-mixing layer. J Fluid Mech 170:83–112Google Scholar
  50. Koochesfahani MM, Mackinnon CG (1991) Influence of forcing on the composition of mixed fluid in a 2-stream shear-layer. Phys Fluids A Fluid Dyn 3(5):1135–1142Google Scholar
  51. Koochesfahani MM, Dimotakis PE, Broadwell JE (1985) A ‘flip’ experiment in a chemically reacting turbulent mixing layer. AIAA J 23(8):1191–1194Google Scholar
  52. Koochesfahani M, Cohn R, McKinnon C (2000) Simultaneous whole-field measurements of velocity and concentration fields using a combination of MTV and LIF. Meas Sci Technol 11(9):1289–1300Google Scholar
  53. Larsen LG, Crimaldi JP (2006) The effect of photobleaching on PLIF. Exp Fluids 41(5):803–812Google Scholar
  54. Law AWK, Wang HW (2000) Measurement of mixing processes with combined digital particle image velocimetry and planar laser induced fluorescence. Exp Therm Fluid Sci 22(3–4):213–229Google Scholar
  55. Lemoine F, Wolff M, Lebouche M (1996) Simultaneous concentration and velocity measurements using combined laser-induced fluorescence and laser doppler velocimetry: application to turbulent transport. Exp Fluids 20(5):319–327Google Scholar
  56. Lemoine F, Antoine Y, Wolff M, Lebouche M (1999) Simultaneous temperature and 2D velocity measurements in a turbulent heated jet using combined laser-induced fluorescence and LDA. Exp Fluids 26(4):315–323Google Scholar
  57. Lempert WR, Magee K, Ronney P, Gee KR, Haugland RP (1995) Flow tagging velocimetry in incompressible-flow using photo-activated nonintrusive tracking of molecular-motion (PHANTOMM). Exp Fluids 18(4):249–257Google Scholar
  58. Liu HT, Lin JT, Delisi DP, Robben FA (1977) Application of a fluorescence technique to dye-concentration measurements in a turbulent jet. In: Proceedings of the symposium on flow measurement in open channels and closed conduits. NBS Special Publication 484, pp 423–446Google Scholar
  59. Matsumoto R, Zadeh HF, Ehrhard P (2005) Quantitative measurement of depth-averaged concentration fields in microchannels by means of a fluorescence intensity method. Exp Fluids 39(4):722–729Google Scholar
  60. Mead K, Wiley M, Koehl M, Koseff J (2003) Fine-scale patterns of odor encounter by the antennules of mantis shrimp tracking turbulent plumes in wave-affected and unidirectional flow. J Exp Biol 206(1):181–193Google Scholar
  61. Melton LA, Lipp CW (2003) Criteria for quantitative PLIF experiments using high-power lasers. Exp Fluids 35(4):310–316Google Scholar
  62. Miller PL, Dimotakis PE (1991) Reynolds-number dependence of scalar fluctuations in a high schmidt number turbulent jet. Phys Fluids A Fluid Dyn 3(5):1156–1163Google Scholar
  63. Moghaddas JS, Tragardh C, Kovacs T, Ostergren K (2002) A new method for measuring concentration of a fluorescent tracer in bubbly gas-liquid flows. Exp Fluids 32(6):728–729Google Scholar
  64. Monismith SG, Koseff JR, Thompson JK, Oriordan CA, Nepf HM (1990) A study of model bivalve siphonal currents. Limnol Oceanogr 35(3):680–696CrossRefGoogle Scholar
  65. Munsterer T, Jahne B (1998) LIF measurements of concentration profiles in the aqueous mass boundary layer. Exp Fluids 25(3):190–196Google Scholar
  66. Nash JD, Jirka GH, Chen D (1995) Large-scale planar laser-induced fluorescence in turbulent density-stratified flows. Exp Fluids 19(5):297–304Google Scholar
  67. Niederhaus CE, Champagne FH, Jacobs JW (1997) Scalar transport in a swirling transverse jet. AIAA J 35(11):1697–1704Google Scholar
  68. Onishi R, Komori S (2006) Thermally stratified liquid turbulence with a chemical reaction. AICHE J 52(2):456–468Google Scholar
  69. Owen F (1976) Simultaneous laser measurement of instantaneous velocity and concentrations in turbulent mixing flows. In: Proceedings of the AGARD conference of non-intrusive instrumentation in fluid flow research, AGARD-CP-193Google Scholar
  70. Pan G, Meng H (2001) Experimental study of turbulent mixing in a tee mixer using PIV and PLIF. AICHE J 47(12):2653–2665Google Scholar
  71. Panchuk-Voloshina N, Haugland RP, BishopñStewart J, Bhalgat MK, Millard PJ, Mao F, Leung WY, Haugland RP (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 47(9):1179–1188Google Scholar
  72. Papanicolaou PN, List EJ (1988) Investigations of round vertical turbulent buoyant jets. J Fluid Mech 195:341–391Google Scholar
  73. Papantoniou D, List EJ (1989) Large-scale structure in the far field of buoyant jets. J Fluid Mech 209:151–190Google Scholar
  74. Parsons JD, Garcia MH (1998) Similarity of gravity current fronts. Phys Fluids 10(12):3209–3213Google Scholar
  75. Patsayeva SV, Yuzhakov VI, Varlamov V (1999) Laser-induced fluorescence saturation for binary mixtures of organic luminophores. In: ICONO ’98: laser spectroscopy and optical diagnostics: novel trends and applications in laser chemistry, biophysics, and biomedicine, SPIE, Moscow, Russia, vol 3732, pp 147–156Google Scholar
  76. Penzkofer A, Leupacher W (1987) Fluorescence behavior of highly concentrated rhodamine 6g solutions. J Lumin 37(2):61–72Google Scholar
  77. Prasad RR, Sreenivasan KR (1990) Quantitative 3-dimensional imaging and the structure of passive scalar fields in fully turbulent flows. J Fluid Mech 216:1–34Google Scholar
  78. Rani SA, Pitts B, Stewart PS (2005) Rapid diffusion of fluorescent tracers into staphylococcus epidermidis biofilms visualized by time lapse microscopy. Antimicrob Agents Chemother 49(2):728–732Google Scholar
  79. Rashidi M, Peurrung L, Tompson AFB, Kulp TJ (1996) Experimental analysis of pore-scale flow and transport in porous media. Adv Water Resour 19(3):163–180Google Scholar
  80. Reynolds O (1883) An experimental investigation of the circumstances which determine whether the motion of water in parallel channels shall be direct or sinuous and of the law of resistance in parallel channels. Philos Trans R Soc 174:935–982Google Scholar
  81. Sakakibara J, Adrian R (1999) Whole field measurement of temperature in water using two-color laser-indiced fluorescence. Exp Fluids 26:7–15Google Scholar
  82. Sakakibara J, Hishida K, Maeda M (1993) Measurements of thermally stratified pipe-flow using image-processing techniques. Exp Fluids 16(2):82–96Google Scholar
  83. Sakakibara J, Hishida K, Maeda M (1997) Vortex structure and heat transfer in the stagnation region of an impinging plane jet (simultaneous measurements of velocity and temperature fields by digital particle image velocimetry and laser-induced fluorescence). Int J Heat Mass Transfer 40(13):3163–3176Google Scholar
  84. Samothrakis P, Cotel AJ (2006) Propagation of a gravity current in a two-layer stratified environment. J Geophys Res Oceans 111(C1):1–17Google Scholar
  85. Saylor J (1995) Photobleaching of disodium fluorescein in water. Exp Fluids 18:445–447Google Scholar
  86. Shan JW, Lang DB, Dimotakis PE (2004) Scalar concentration measurements in liquid-phase flows with pulsed lasers. Exp Fluids 36(2):268–273Google Scholar
  87. Shiono K, Feng T (2003) Turbulence measurements of dye concentration and effects of secondary flow on distribution in open channel flows. J Hydraul Eng ASCE 129(5):373–384Google Scholar
  88. Shlien DJ (1988) Instantaneous concentration field measurement technique from flow visualization photographs. Exp Fluids 6(8):541–546Google Scholar
  89. Shy SS, Breidenthal RE (1991) Turbulent stratified interfaces. Phys Fluids A Fluid Dyn 3(5):1278–1285Google Scholar
  90. Simoens S, Ayrault M (1994) Concentration flux measurements of a scalar quantity in turbulent flows. Exp Fluids 16(3-4):273–281Google Scholar
  91. Smart P, Laidlaw I (1977) An evaluation of some fluorescent dyes for water tracing. Water Resour Res 13:15–33Google Scholar
  92. Sreenivasan KR, Prasad RR (1989) New results on the fractal and multifractal structure of the large schmidt number passive scalars in fully turbulent flows. Physica D 38(1–3):322–329Google Scholar
  93. Stapountzis H, Westerweel J, Bessem JM, Westendorp A, Nieuwstadt FTM (1992) Measurement of product concentration of 2 parallel reactive jets using digital image-processing. Appl Sci Res 49(3):245–259Google Scholar
  94. Stohr M, Roth K, Jahne B (2003) Measurement of 3D pore-scale flow in index-matched porous media. Exp Fluids 35(2):159–166Google Scholar
  95. Su LK, Dahm WJA (1996) Scalar imaging velocimetry measurements of the velocity gradient tensor field in turbulent flows. 2. Experimental results. Phys Fluids 8(7):1883–1906Google Scholar
  96. Tian XD, Roberts PJW (2003) A 3D LIF system for turbulent buoyant jet flows. Exp Fluids 35(6):636–647Google Scholar
  97. Tokumaru PT, Dimotakis PE (1995) Image correlation velocimetry. Exp Fluids 19(1):1–15Google Scholar
  98. Troy C, Koseff JR (2005a) The generation and quantitative visualization of breaking internal waves. Exp Fluids 38:549–562Google Scholar
  99. Troy CD, Koseff JR (2005b) The instability and breaking of long internal waves. J Fluid Mech 543:107–136MATHGoogle Scholar
  100. Unger DR, Muzzio FJ (1999) Laser-induced fluorescence technique for the quantification of mixing in impinging jets. AICHE J 45(12):2477–2486Google Scholar
  101. Van Cruyningen I, Lozano A, Hanson RK (1990) Quantitative imaging of concentration by planar laser-induced fluorescence. Exp Fluids 10(1):41–49Google Scholar
  102. Van Vliet E, Van Bergen SM, Derksen JJ, Portela LM, Van den Akker HEA (2004) Time-resolved, 3D, laser-induced fluorescence measurements of fine-structure passive scalar mixing in a tubular reactor. Exp Fluids 37(1):1–21Google Scholar
  103. Wadley R, Dawson MK (2005) LIF measurements of blending in static mixers in the turbulent and transitional flow regimes. Chem Eng Sci 60(8–9):2469–2478Google Scholar
  104. Wagner C, Kuhn S, von Rohr PR (2007) Scalar transport from a point source in flows over wavy walls. Exp Fluids 43(2–3):261–271Google Scholar
  105. Walker D (1987) A fluorescence technique for measurement of concentration in mixing liquids. J Phys E Sci Instrum 20:217–224Google Scholar
  106. Wang GR, Fiedler HE (2000a) On high spatial resolution scalar measurement with LIF—part 1: photobleaching and thermal blooming. Exp Fluids 29(3):257–264Google Scholar
  107. Wang GR, Fiedler HE (2000b) On high spatial resolution scalar measurement with LIF—part 2: the noise characteristic. Exp Fluids 29(3):265–274Google Scholar
  108. Ware B, Cyr D, Gorti S, Lanni F (1983) Electrophoretic and frictional properties of particles in complex media measured by laser light scattering and fluorescence photobleaching recovery. In: Dahneke BE (ed) Measurement of suspended particles by quasi-elastic light scattering. Wiley, New York, p 200Google Scholar
  109. Webster DR, Roberts PJW, Ra’ad L (2001) Simultaneous DPTV/PLIF measurements of a turbulent jet. Exp Fluids 30(1):65–72Google Scholar
  110. Webster DR, Rahman S, Dasi LP (2003) Laser-induced fluorescence measurements of a turbulent plume. J Eng Mech ASCE 129(10):1130–1137Google Scholar
  111. Westerweel J, Hofmann T, Fukushima C, Hunt JCR (2002) The turbulent/non-turbulent interface at the outer boundary of a self-similar turbulent jet. Exp Fluids 33(6):873–878Google Scholar
  112. Yoda M, Fiedler HE (1996) The round jet in a uniform counterflow: Flow visualization and mean concentration measurements. Exp Fluids 21(6):427–436Google Scholar
  113. Yoda M, Hesselink L, Mungal MG (1994) Instantaneous 3-dimensional concentration measurements in the self-similar region of a round high-schmidt-number jet. J Fluid Mech 279:313–350Google Scholar
  114. Yu SCM, Law AWK, Ai JJ (2007) Vortex formation process in gravity-driven starting jets. Exp Fluids 42(5):783–797Google Scholar

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© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Civil and Environmental EngineeringUniversity of ColoradoBoulderUSA

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