Quantitative measurement of depth–averaged concentration fields in microchannels by means of a fluorescence intensity method
- First Online:
We have inferred and verified an optical measuring technique, based on fluorescence intensity, for the species concentration field in a liquid microchannel flow. In contrast to macroscopic flows, where a light sheet is usually applied, the complete illumination of the measuring volume in the microchannel provides a height-averaged concentration field. The technique was verified with aqueous solutions within a square glass microchannel of 110 μm width. The verification experiments confirm both a good spatial resolution and a good accuracy of even the concentration gradients within a concentration boundary layer.
- Freitag A, Dietrich T, Scholz R (2000) Glass as a material for microreaction technology. In: Proceedings of IMRET 4, Topical Conference, pp 48–54Google Scholar
- Meisel I, Ehrhard P (2005) Electrically-excited (electroosmotic) flows in microchannels for mixing applications. Eur J Mech B/Fluids (in press)Google Scholar
- Rost F (1991) Quantitative fluorescence microscopy. Cambridge University Press, CambridgeGoogle Scholar
- Rost F (1992) Fluorescence microscopy, vol 1. Cambridge University Press, CambridgeGoogle Scholar