Experiments in Fluids

, Volume 37, Issue 1, pp 105–119 | Cite as

Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM)

  • Jae Sung Park
  • Chang Kyoung Choi
  • Kenneth D. KihmEmail author


Optically sliced microscopic-particle image velocimetry (micro-PIV) is developed using confocal laser scanning microscopy (CLSM). The developed PIV system shows a unique optical slicing capability allowing true depth-wise resolved micro-PIV vector field mapping. A comparative study between CLSM micro-PIV and conventional epi-fluorescence micro-PIV is presented. Both techniques have been applied to the creeping Poiseuille flows in two different microtubes of 99-μm (Re=0.00275) and 516-μm ID diameters (Re=0.021), which are respectively imaged by a 40×-0.75NA objective with an estimated 2.8-μm optical slice thickness, and by a 10×-0.30NA objective with a 26.7-μm slicing. Compared to conventional micro-PIV, CLSM micro-PIV consistently shows significantly improved particle image contrasts, definitions, and measured flow vector fields agreeing more accurately with predictions based on the Poiseuille flow fields. The data improvement due to the optical slicing of CLSM micro-PIV is more pronounced with higher magnification imaging with higher NA objectives for a smaller microtube.


Particle Image Velocimetry Confocal Laser Scanning Microscopy Point Spread Function Particle Image Velocimetry Image Interrogation Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The confocal laser scanning microscope (CLSM) system was purchased by the Texas A&M Permanent University Facility (PUF) Award granted to Dr. Kihm’s Micro/nano-scale Fluidics and Heat Transport Laboratory The authors acknowledge that the current research has been partially sponsored by the NASA-Fluid Physics Research Program, grant no. NAG 3–2712, and partially by a subcontract from the R4D Program at the National Center for Microgravity Research (NCMR). The presented technical contents are not necessarily the representative views of NASA or NCMR.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Jae Sung Park
    • 1
  • Chang Kyoung Choi
    • 1
  • Kenneth D. Kihm
    • 1
    Email author
  1. 1.Micro/Nano-scale Fluidics and Heat Transport Laboratory, Department of Mechanical EngineeringTexas A&M UniversityUSA

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