Der Ophthalmologe

, Volume 110, Issue 7, pp 622–628 | Cite as

Kurz- und Langzeitkomplikationen nach Transplantation von kultiviertem Limbusepithel

  • D. Meller
  • H. Thomasen
  • M.-S. Hanet
  • D. Dekowski
  • S. Scholz
  • K. Amir
  • K.-P. Steuhl
Leitthema

Zusammenfassung

Die in den letzten Jahren erzielten Fortschritte im Tissue-Engineering ermöglichen die Rekonstruktion des okulären Oberflächenepithels. Grenzen und Möglichkeiten der Kultivierung von Limbusepithel als auch Kurz- und Langzeitkomplikationen nach Ex-vivo-Expansion von Limbusepithel zur Behandlung der limbalen Stammzellinsuffizienz werden in dieser Übersichtsarbeit dargestellt.

Schlüsselwörter

Limbusstammzellinsuffizienz Ex-vivo-Expansion Amnionmembran Komplikationen Tissue-Engineering 

Short-term and long-term complications after transplantation of cultivated limbal epithelium

Abstract

Recent advances in tissue engineering have facilitated the development of new strategies in ocular surface reconstruction. Limitations and possibilities of ex vivo cultivation and limbal epithelium cell culture techniques as well as the short and long-term complications after transplantation of ex vivo expansion of cultivated limbal epithelium for the treatment of limbal stem cell deficiency are summarized in this review.

Keywords

Limbal stem cell deficiency Tissue engineering Ex vivo expansion Amniotic membrane Complications 

Literatur

  1. 1.
    Ban Y, Cooper LJ, Fullwood NJ et al (2003) Comparison of ultrastructure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting. Exp Eye Res 76:735–743PubMedCrossRefGoogle Scholar
  2. 2.
    Cotsarelis G, Cheng SZ, Dong G et al (1989) Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell 57:201–209PubMedCrossRefGoogle Scholar
  3. 3.
    Davanger M, Evensen A (1971) Role of the pericorneal papillary structure in renewal of corneal epithelium. Nature 229:560–561PubMedCrossRefGoogle Scholar
  4. 4.
    Grueterich M, Espana EM, Tseng SC (2003) Ex vivo expansion of limbal epithelial stem cells: amniotic membrane serving as a stem cell niche. Surv Ophthalmol 48:631–646PubMedCrossRefGoogle Scholar
  5. 5.
    Hahn A, Thanos M, Reinhard T et al (2010) Procedural guidelines. Good practice procedures for acquisition and preparation of cryopreserved human amniotic membranes from donor placentas. Ophthalmologe 107:1020–1031PubMedCrossRefGoogle Scholar
  6. 6.
    Hernandez Galindo EE, Theiss C, Steuhl KP et al (2003) Expression of Delta Np63 in response to phorbol ester in human limbal epithelial cells expanded on intact human amniotic membrane. Invest Ophthalmol Vis Sci 44:2959–2965CrossRefGoogle Scholar
  7. 7.
    Hernandez Galindo EE, Theiss C, Steuhl KP et al (2003) Gap junctional communication in microinjected human limbal and peripheral corneal epithelial cells cultured on intact amniotic membrane. Exp Eye Res 76:303–314CrossRefGoogle Scholar
  8. 8.
    Kenyon KR, Tseng SC (1989) Limbal autograft transplantation for ocular surface disorders. Ophthalmology 96:709–722 (discussion 722–703)PubMedGoogle Scholar
  9. 9.
    Koizumi N, Inatomi T, Suzuki T et al (2001) Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 108:1569–1574PubMedCrossRefGoogle Scholar
  10. 10.
    Koizumi N, Rigby H, Fullwood NJ et al (2007) Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells. Graefes Arch Clin Exp Ophthalmol 245:123–134PubMedCrossRefGoogle Scholar
  11. 11.
    Lehrer MS, Sun TT, Lavker RM (1998) Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation. J Cell Sci 111(Pt 19):2867–2875PubMedGoogle Scholar
  12. 12.
    Meller D, Pauklin M, Westekemper H et al (2010) Autologous transplantation of cultivated limbal epithelium. Ophthalmologe 107:1133–1138PubMedCrossRefGoogle Scholar
  13. 13.
    Meller D, Pires RT, Tseng SC (2002) Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol 86:463–471PubMedCrossRefGoogle Scholar
  14. 14.
    Meller D, Thomasen H, Steuhl KP (2012) Ocular surface reconstruction in limbal stem cell deficiency: transplantation of cultivated limbal epithelium. Ophthalmologe 109:863–868PubMedCrossRefGoogle Scholar
  15. 15.
    Pauklin M, Fuchsluger TA, Westekemper H et al (2010) Midterm results of cultivated autologous and allogeneic limbal epithelial transplantation in limbal stem cell deficiency. Dev Ophthalmol 45:57–70PubMedCrossRefGoogle Scholar
  16. 16.
    Pauklin M, Thomasen H, Pester A et al (2011) Expression of pluripotency and multipotency factors in human ocular surface tissues. Curr Eye Res 36:1086–1097PubMedCrossRefGoogle Scholar
  17. 17.
    Pellegrini G, Traverso CE, Franzi AT et al (1997) Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 349:990–993PubMedCrossRefGoogle Scholar
  18. 18.
    Resch MD, Schlotzer-Schrehardt U, Hofmann-Rummelt C et al (2006) Integration patterns of cryopreserved amniotic membranes into the human cornea. Ophthalmology 113:1927–1935PubMedCrossRefGoogle Scholar
  19. 19.
    Santos MS, Gomes JA, Hofling-Lima AL et al (2005) Survival analysis of conjunctival limbal grafts and amniotic membrane transplantation in eyes with total limbal stem cell deficiency. Am J Ophthalmol 140:223–230PubMedCrossRefGoogle Scholar
  20. 20.
    Schermer A, Galvin S, Sun TT (1986) Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 103:49–62PubMedCrossRefGoogle Scholar
  21. 21.
    Schwab IR, Reyes M, Isseroff RR (2000) Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea 19:421–426PubMedCrossRefGoogle Scholar
  22. 22.
    Shortt AJ, Secker GA, Notara MD et al (2007) Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results. Surv Ophthalmol 52:483–502PubMedCrossRefGoogle Scholar
  23. 23.
    Thomasen H, Steuhl KP, Meller D (2012) The biological basis of limbal stem cell deficiency. Ophthalmologe 109:843–849PubMedCrossRefGoogle Scholar
  24. 24.
    Tsai RJ, Li LM, Chen JK (2000) Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med 343:86–93PubMedCrossRefGoogle Scholar
  25. 25.
    Tseng SC (1989) Concept and application of limbal stem cells. Eye (Lond) 3(Pt 2):141–157Google Scholar
  26. 26.
    Tseng SC, Prabhasawat P, Barton K et al (1998) Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency. Arch Ophthalmol 116:431–441PubMedCrossRefGoogle Scholar
  27. 27.
    Tseng SC, Tsubota K (1997) Important concepts for treating ocular surface and tear disorders. Am J Ophthalmol 124:825–835PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • D. Meller
    • 1
  • H. Thomasen
    • 1
  • M.-S. Hanet
    • 1
  • D. Dekowski
    • 1
  • S. Scholz
    • 1
  • K. Amir
    • 1
  • K.-P. Steuhl
    • 1
  1. 1.Klinik für Erkrankungen des vorderen Augenabschnitts, Zentrum für AugenheilkundeUniversität Duisburg-EssenEssenDeutschland

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