Der Ophthalmologe

, Volume 101, Issue 10, pp 993–997 | Cite as

Carboxyamido-triazol (CAI) inhibiert Unterschritte der choroidalen Neovaskularisation an choroidalen Endothelzellen und retinalen Pigmentepithelzellen in vitro

Originalien
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Zusammenfassung

Ziel:

Retinale Pigmentepithelzellen (RPEs) und choroidale Endothelzellen (CECs) spielen eine wichtige Rolle bei der Ausbildung einer choroidalen Neovaskularisation im Rahmen der altersbedingten exsudativen Makuladegeneration (AMD). In dieser Studie untersuchen wir den Einfluss der die Kalziumsignaltransduktion modulierenden Substanz Carboxyamido-triazole (CAI) auf die Proliferation und Migration dieser Zellarten.

Methoden:

Humane fetale RPE-Zellen und bovine CECs wurden mit CAI in einem Konzentrationsbereich von 0,1–10 µM inkubiert. Die Effekte des CAI auf Serum- sowie „basic fibroblast growth factor“- (bFGF) (10 ng/ml) stimulierte Proliferation dieser Zellarten wurden bestimmt. Zusätzlich wurde der Einfluss des CAI auf die Zellmigration von CECs und RPE-Zellen untersucht.

Ergebnisse:

CAI inhibiert die Proliferation von Serum-stimulierten CECs stärker als die RPE-Zellproliferation in einer Konzentration von 10 µM. Noch ausgeprägter inhibiert CAI die bFGF-stimulierte RPE-Zell- und CEC-Proliferation. Auch die fibronektininduzierte RPE-Zell- und CEC-Migration konnte durch CAI inhibiert werden, wobei die Migration von RPE-Zellen stärker gehemmt wurde als die Migration von CECs.

Schlussfolgerung:

CAI inhibiert wichtige Subschritte der choroidalen Neovaskularisation wie Proliferation und Migration von RPE-Zellen und CECs. CAI könnte somit von Bedeutung sein für die Hemmung der choroidalen Neovaskularisation bei der exsudativen Makuladegeneration.

Schlüsselwörter

Choroidale Neovaskularisation Altersabhängige Makuladegeneration Signaltransduktionstherapie Choroidale Endothelzellen Retinale Pigmentepithelzellen 

Carboxyamido-triazole inhibits substeps of choroidal neovascularization on retinal pigment epithelial cells and choroidal endothelial cells in vitro

Abstract

Background

Retinal pigment epithelial cells (RPE cells) and choroidal endothelial cells (CECs) are important cell types in the process of choroidal neovascularization in exudative age-related macular degeneration (AMD). In this study, we evaluated the antiproliferative and antimigratory abilities of carboxyamido-triazole (CAI), a drug modulating calcium-dependent signal transduction on RPE cells and CECs.

Methods

Human fetal RPE cells and bovine CECs were exposed to CAI in a concentration range of 0.1 to 10 µM. Cell proliferation was stimulated with 10% serum or 10 ng/ml bFGF. The effect of CAIs on cell proliferation was estimated. Furthermore, we evaluated CAI’s effects on CEC and RPE cell migration induced by fibronectin.

Results

CAI had a stronger inhibitory effect on serum-induced CEC proliferation than on RPE cell proliferation. A much stronger effect was seen on the proliferation of bFGF-stimulated RPE cells and CECs. Furthermore, the fibronectin-stimulated migration of RPE cells and CECs was inhibited by CAI. In this assay, a stronger inhibitory effect was seen on RPE cells than on CECs.

Conclusion

CAI inhibits important substeps of choroidal neovascularization on RPR cells and CECs. Therefore, CAI may be of value for the treatment of choroidal neovascularization in exudative AMD.

Keywords

Choroidal neovascularization Age-related macular degeneration Signal transduction therapy Choroidal endothelial cell Retinal pigment epithelial cell 
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Notes

Interessenkonflikt:

Keine Angaben

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Universitätsaugenklinik LeipzigLeipzig
  2. 2.Doheny Eye InstituteUniversity of Southern California, USC
  3. 3.Universitätsaugenklinik LeipzigLeipzig

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