World Journal of Urology

, Volume 31, Issue 1, pp 241–246

Mapping the cytokine profile of painful bladder syndrome/interstitial cystitis in human bladder and urine specimens

  • Anthony T. Corcoran
  • Naoki Yoshimura
  • Vikas Tyagi
  • Bruce Jacobs
  • Wendy Leng
  • Pradeep Tyagi
Original Article

DOI: 10.1007/s00345-012-0852-y

Cite this article as:
Corcoran, A.T., Yoshimura, N., Tyagi, V. et al. World J Urol (2013) 31: 241. doi:10.1007/s00345-012-0852-y

Abstract

Purpose

This study investigated the cytokine profile in bladder tissue and urine of painful bladder syndrome/interstitial cystitis (PBS/IC) patients.

Methods

Multiplex analysis of 23 cytokines was performed with a multiple antigen bead assay (Luminex 100 IS) on cold cup bladder biopsy and urine specimens collected during cystoscopy with hydrodistention (HD) under general anesthesia from 10 PBS/IC patients (ICS definition). Collected tissue specimens and urine from pre-HD and post-HD (mean 27 days) were compared to banked urine and tissue specimens (n = 10) collected from control subjects without PBS/IC symptoms.

Results

Univariate comparison of bladder tissue levels found significant elevation of IL-16, IL-18, CTACK, ICAM-1, MCP-3, SCGFβ, TRAIL, and VCAM-1 in PBS/IC relative to controls. Multivariate analysis revealed VCAM-1 and ICAM-1 were responsible for the discrimination of both tissue and urine of PBS/IC from controls. Urine levels of MCP-3 and TRAIL were significantly reduced a month after HD in concert with improvement in standardized measures of clinical symptoms (pain, urgency, and frequency (PUF) overall score [mean 25.8 ± 5.5 vs. 20.3 ± 7, p = 0.04] and symptom score [mean 18.2 ± 3.2 vs. 12.2 ± 5.9; p = 0.009]). Post-HD urine levels of MCSF(r = 0.88; p = 0.003), MCP-3 (r = 0.81; p = 0.01), SDF1α (r = 0.82; p = 0.01), and IL-18 (r = 0.64; p = 0.08) positively correlated with improved symptom scores.

Conclusions

These results indicate significant elevation of cytokines in PBS/IC bladder tissue relative to controls. Significant reduction in post-HD urine levels of MCP-3 and TRAIL relative to pre-HD in PBS/IC was associated with clinical improvement (as measured by PBS/IC symptom scores) to qualify them as biomarker candidates.

Keywords

Cytokines Interstitial cystitis Urine Bladder Biomarker 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Anthony T. Corcoran
    • 1
  • Naoki Yoshimura
    • 1
  • Vikas Tyagi
    • 1
  • Bruce Jacobs
    • 1
  • Wendy Leng
    • 1
  • Pradeep Tyagi
    • 1
  1. 1.Department of UrologyUniversity of Pittsburgh School of MedicinePittsburghUSA

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