World Journal of Urology

, Volume 28, Issue 4, pp 465–471 | Cite as

Maspin modulates adhesion of bladder carcinoma cells to vascular endothelium

  • Eva Juengel
  • Wolf-Dietrich C. Beecken
  • Santhosh Mundiyanapurath
  • Tobias Engl
  • Dietger Jonas
  • Roman A. Blaheta
Original Article

Abstract

Purpose

Maspin belongs to the serpin family and has been shown to suppress tumor growth and metastasis in several tumor types. The role of maspin in bladder carcinoma has not been fully elucidated, and the object of this study was to investigate whether maspin contributes to bladder tumor adhesion to vascular endothelial cells (HUVEC).

Methods

Expression of maspin-coding mRNA was evaluated in a panel of bladder carcinoma cell lines. Maspin distribution in maspin mRNAhigh versus maspin mRNAlow cells was further analyzed by flow cytometry and confocal microscopy. Adhesion to HUVEC was measured in a coculture model and correlated with the surface-bound maspin.

Results

Maspinhigh (RT-4, RT-112) cell lines strongly attached to HUVEC, whereas maspinlow (UMUC-3, MGH-U1) cell lines poorly adhered to HUVEC. Distinct cytoplasmic maspin accumulation and moderate surface-bound maspin was found in RT-4 cells. Blocking maspin surface receptors prevented tumor cell attachment to HUVEC, indicating that surface-bound maspin is responsible for triggering cell adhesion. PMA-triggered elevation of surface-bound maspin was accompanied by an enhanced adhesion capacity of RT-4 cells, compared to controls. Finally, exposing the bladder carcinoma cells to the differentiation-inducing agent valproic acid led to a surface-bound (but not cytoplasmic) maspin decrease, paralleled by a significant reduction in tumor cell binding to HUVEC.

Conclusion

Surface-bound maspin directly controls bladder carcinoma cell adhesion to the vascular wall. Blocking this process may prevent transendothelial migration and tumor cell dissemination. Therefore, therapeutic down-regulation of surface-bound maspin might become an option to prevent tumor spread into distant organs.

Keywords

Adhesion Bladder carcinoma HUVEC Maspin Valproic acid 

Notes

Acknowledgments

We would like to thank Karen Nelson for critically reading the manuscript. This work was supported by the “Horst Müggenburg-Stiftung”.

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Eva Juengel
    • 1
  • Wolf-Dietrich C. Beecken
    • 1
  • Santhosh Mundiyanapurath
    • 1
  • Tobias Engl
    • 1
  • Dietger Jonas
    • 1
  • Roman A. Blaheta
    • 1
  1. 1.Klinik für Urologie und Kinderurologie, Zentrum der ChirurgieJohann Wolfgang Goethe-UniversitätsklinikFrankfurt am MainGermany

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