Protective role of erythropoietin during testicular torsion of the rats
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Testicular torsion is an important clinical urgency. Similar mechanisms occurred after detorsion of the affected testis as in the ischemia reperfusion (I/R) damage. This study was designed to investigate the effects of erythropoietin (EPO) treatment after unilateral testicular torsion. Fifty male Sprague-Dawley rats were divided into five groups. Group 1 underwent a sham operation of the right testis under general anesthesia. Group 2 was same as sham, and EPO (3,000 IU/kg) infused i.p., group 3 underwent a similar operation but the right testis was rotated 720° clockwise for 1 h, maintained by fixing the testis to the scrotum, and saline infused during the procedure. Group 4 underwent similar torsion but EPO was infused half an hour before the detorsion procedure, and in group 5, EPO was infused after detorsion procedure. Four hours after detorsion, ipsilateral and contralateral testes were taken out for evaluation. Treatment with EPO improved testicular structures in the ipsilateral testis but improvement was less in the contralateral testis histologically, but EPO treatment decreased germ cell apoptosis in both testes following testicular IR. TNF-α, IL-1β, IL-6 and nitrite levels decreased after EPO treatment especially in the ipsilateral testis. We conclude that testicular I/R causes an increase in germ cell apoptosis both in the ipsilateral and contralateral testes. Eryhropoietin has antiapoptotic and anti-inflammatory effects following testicular torsion.
KeywordsTesticular torsion Erythropoietin Apoptosis Cytokine Nitrite
This study was supported by TUBITAK (The Scientific and Technological Research Council of Turkey) project no: 104S412 (SBAG-AYD-487).
- 12.Tunctan B, Abacioglu N (1998) J Pharm Sci 23:161–170Google Scholar
- 17.Zoja C, Wang JM, Bettoni S, Sironi M, Renzi D, Chiaffarino F, Abboud HE, Van Damme J, Mantovani A, Remuzzi G et al (1991) Interleukin-1 beta and tumor necrosis factor-alpha induce gene expression and production of leukocyte chemotactic factors, colony-stimulating factors, and interleukin-6 in human mesangial cells. Am J Pathol 138(4):991–1003PubMedGoogle Scholar
- 19.Vera Y, Erkkila K, Wang C, Nunez C, Kyttanen S, Lue Y, Dunkel L, Swerdloff RS, Sinha Hikim AP (2006) Involvement of p38 mitogen-activated protein kinase and inducible nitric oxide synthase in apoptotic signaling of murine and human male germ cells after hormone deprivation. Mol Endocrinol 20(7):1597–1609PubMedCrossRefGoogle Scholar
- 20.Basar MM, Kisa U, Tuglu D, Yilmaz E, Basar H, Caglayan O, Batislam E (2006) Testicular nitric oxide and thiobarbituric acid reactive substances levels in obstructive azoospermia: a possible role in pathophysiology of infertility. Mediators Inflamm 3:27458Google Scholar
- 22.Meroni SB, Subuo AM, Cigorraga SB (2000) Interleukin-1beta regulates nitric oxide production and gamma-glutamyl transpeptidase activity in sertoli cells. Androl 21(6):855–861Google Scholar