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Cladosporium cladosporioides C24G Modulates Gene Expression and Enzymatic Activity During Leaf Blast Suppression in Rice Plants

  • Amanda Abdallah Chaibub
  • Thatyane Pereira de Sousa
  • Leila Garcês de Araújo
  • Marta Cristina Corsi de FilippiEmail author
Article

Abstract

The inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization—preventive and 4: foliar spray pulverization—curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), β-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and decreasing environmental contaminations.

Keywords

Biocontrol Oryza sativa Induced resistance Real-time PCR PR proteins 

Notes

Supplementary material

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Supplementary file1 (PDF 26 kb)
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Supplementary file2 (PDF 29 kb)
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Supplementary file3 (PDF 237 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant PathologyUniversity of BrasíliaBrasíliaBrazil
  2. 2.Agronomy SchoolFederal University of GoiásGoiâniaBrazil
  3. 3.Microorganisms Genetics LaboratoryFederal University of GoiásGoiâniaBrazil
  4. 4.Agricultural Microbiology LaboratoryEmbrapa Rice and BeansSanto Antônio de GoiásBrazil

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