Silencing of the MADS-Box Gene SlMADS83 Enhances Adventitious Root Formation in Tomato Plants

  • Anzhou Li
  • Guoping Chen
  • Yunshu Wang
  • Honglian Liang
  • Zongli HuEmail author


Adventitious roots (ARs) are important for the growth of plants and the improvement in their stress resistance and survival capacity. Although many genes have been confirmed to be involved in adventitious root (AR) formation in Arabidopsis and tomato plants, MADS-box genes have rarely been mentioned. Here, we isolated a MADS-box gene named SlMADS83, which may negatively regulate AR formation in tomato plants, as the number of the ARs formed in the transgenic lines in which the SlMADS83 gene was silenced by RNA interference (RNAi) was increased. The above phenotype was further confirmed by the analysis of the macroscopic, anatomical, and molecular features and related statistical data. Previous Studies have proven that auxin can stimulate early AR primordium initiation. Interestingly, in the RNAi transgenic lines, the concentration of auxin in the hypocotyl base was increased, resulting in early induction of AR primordia initiation, promoting the formation of ARs. Briefly, SlMADS83 may play an important role in AR formation.


Adventitious roots Tomato SlMADS83 Auxin biosynthesis 



RNA interference


Indole-3-acetic acid





This work was supported by National Natural Science Foundation of China (No. 31572129) and the Natural Science Foundation of Chongqing of China (No. cstc2015jcyjA80026).

Author Contributions

Z.H. and G.C. designed the research; A.L., Y.W., and H.L. performed the research; A.L. wrote the paper; Z.H. modified the paper. All authors have read and approved the final version of the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

344_2019_10035_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)
344_2019_10035_MOESM2_ESM.docx (433 kb)
Supplementary material 2 (DOCX 433 kb) Supplementary Fig. S1. Phylogenetic analyses and gene expression prediction.
344_2019_10035_MOESM3_ESM.docx (20 kb)
Supplementary material 3 (DOCX 19 kb) Supplementary Table S1. Specific primer sequences used in this study.
344_2019_10035_MOESM4_ESM.docx (16 kb)
Supplementary material 4 (DOCX 16 kb) Supplementary Table S2. Relative potential MADS binding sites (GArG motifs) in the promoter sequences.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Anzhou Li
    • 1
  • Guoping Chen
    • 1
  • Yunshu Wang
    • 1
  • Honglian Liang
    • 1
  • Zongli Hu
    • 1
    • 2
    Email author
  1. 1.Laboratory of Molecular Biology of Tomato, Bioengineering CollegeChongqing UniversityChongqingPeople’s Republic of China
  2. 2.Bioengineering CollegeChongqing UniversityChongqingPeople’s Republic of China

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