Advertisement

Physiological effects of different concentrations of chloride deicing salt and freeze–thaw stress on Secale cereale L. seedlings

  • Guozhang BaoEmail author
  • Fanglin He
  • Weiwei Chen
  • Jiaxing Sun
  • Xuemei Ding
Article
  • 104 Downloads

Abstract

Chloride deicing salt stress usually coincides with the event of freeze–thaw, and the short-term adaptation of Dongmu-70 Secale cereale L. seedlings to these stresses was investigated in this paper. The chloride deicing salt and the freeze–thaw (FT) simulation experiments were carried out in the lab and alternation refrigerator. The changes of soluble sugar, soluble protein, relative conductivity (RC), malondialdehyde (MDA), and catalase (CAT) activity in seedlings were studied under freeze–thaw stress (10, 5, 0, − 5, 0, 5, and 10 °C) and 0, 200, 400, and 600 mmol L−1 of chloride deicing salt stress (CK, D1, D2, and D3). The results indicated that the content of physiological index in different treatment groups rose first and then decreased within a freeze–thaw cycle. During the recovery phase (T8: 24 h after freeze–thaw stress and T9: 6 days after freeze–thaw stress), there was significant difference either in MDA and CAT activity between D2 × FT and D2 or in RC, MDA, soluble sugar, and CAT activity between D3 × FT and D3. The seedlings showed different adaptability under different intensities of combined stress, and the sequence of the changes in physiological index can be patterned as D × FT > FT > D > CK. Freeze–thaw and chloride deicing salt complex stress exhibited a synergistic effect on the plant, which indicates that the snow-melting operation would be more harmful in spring and autumn to plants than in winter.

Keywords

Secale cereale L. Chloride deicing salt Freeze–thaw Physiological response 

Notes

Acknowledgements

Financial support from the National Natural Science Foundation of China (Grant No. 31772669) to Professor Bao is gratefully acknowledged.

Funding

This work was sponsored by the National Natural Science Foundation of China (Grant No. 31772669).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Augustyniak A, Perlikowski D, Rapacz M, Koscielniak J, Kosmala A (2018) Insight into cellular proteome of Lolium multiflorum/Festuca arundinacea introgression forms to decipher crucial mechanisms of cold acclimation in forage grasses. Plant Sci 272:22–31.  https://doi.org/10.1016/j.plantsci.2018.04.002 CrossRefGoogle Scholar
  2. Bao G, Ao Q, Li Q, Bao Y, Zheng Y, Feng X, Ding X (2017) Physiological characteristics of Medicago sativa L. in response to acid deposition and freeze-thaw stress. Water Air Soil Pollut 228:376–386.  https://doi.org/10.1007/s11270-017-3561-8 CrossRefGoogle Scholar
  3. Barbier L, Suaire R, Durickovic I, Laurent J, Simonnot MO (2018) Is a road stormwater retention pond able to intercept deicing salt? Water Air Soil Pollution 229:251–263.  https://doi.org/10.1007/s11270-018-3908-9 CrossRefGoogle Scholar
  4. Bertazzini M, Sacchi GA, Forlani G (2018) A differential tolerance to mild salt stress conditions among six Italian rice genotypes does not rely on Na+ exclusion from shoots. J Plant Physiol 226:145–153.  https://doi.org/10.1016/j.jplph.2018.04.011 CrossRefGoogle Scholar
  5. Bian W, Bao G, Qian H, Song Z, Qi Z, Zhan M, Chen W, Dong W (2018) Physiological response characteristics in Medicago sativa under freeze-thaw and deicing salt stress. Water Air Soil Pollut 229:196–203.  https://doi.org/10.1007/s11270-018-3850-x CrossRefGoogle Scholar
  6. Blomqvist G, Johansson EL (1999) Airborne spreading and deposition of de-icing salt—a case study. Sci Total Environ 235:161–168.  https://doi.org/10.1016/s0048-9697(99)00209-0 CrossRefGoogle Scholar
  7. Blomqvist G, Gustafsson M, Eram M, Unver K (2011) Prediction of salt on road surface tool to minimize use of salt. Transp Res Rec 2258:131–138.  https://doi.org/10.3141/2258-16 CrossRefGoogle Scholar
  8. Bothe A, Westermeier P, Wosnitza A, Willner E, Schum A, Dehmer KJ, Hartmann S (2018) Drought tolerance in perennial ryegrass (Lolium perenne L.) as assessed by two contrasting phenotyping systems. J Agron Crop Sci 204:375–389.  https://doi.org/10.1111/jac.12269 CrossRefGoogle Scholar
  9. Chen J, Wang X (2006) Plant physiology experiment, 2nd edn. South China University of Technology, Guangzhou, pp 64–66 (in Chinese)Google Scholar
  10. Chen J, Tao L, Zhu W (2004) Biochemical experiment, 3rd edn. Science, Beijing, pp 13–14 (in Chinese)Google Scholar
  11. Chen S, Chen T, Yao X, Lv H, Li C (2018) Physicochemical properties of an asexual epichloe endophute-modified wild barley in the presence of salt stress. Pak J Bot 50:2105–2111Google Scholar
  12. Coiner HA, Hayhoe K, Ziska LH, Van Dorn J, Sage RF (2018) Tolerance of subzero winter cold in kudzu (Pueraria montana var. lobata). Oecologia 187:839–849.  https://doi.org/10.1007/s00442-018-4157-8 CrossRefGoogle Scholar
  13. Fleck RA, Day JG, Clarke KJ, Benson EE (1999) Elucidation of the metabolic and structural basis for the cryopreservation recalcitrance of Vaucheria sessilis. Cryo-Letters 20:271–282Google Scholar
  14. Gao Z, Hu Z, Jha B, Yang S, Zhu J, Shen B, Zhang R (2014) Variability and predictability of Northeast China climate during 1948–2012. Clim Dyn 43:787–804.  https://doi.org/10.1007/s00382-013-1944-0 CrossRefGoogle Scholar
  15. He L, Xu X, Liu F, Liu X (2011) Effects of NaCl stress on physio-ecological responses of Glycine soja. Soybean Sci 30:242–245.  https://doi.org/10.11861/j.issn.1000-9841.2011.02.0242 (in Chinese).Google Scholar
  16. He A, Niu S, Zhao Q, Li Y, Gou J, Gao H, Suo S, Zhang J (2018) Induced salt tolerance of perennial ryegrass by a novel bacterium strain from the rhizosphere of a desert shrub haloxylon ammodendron. Int J Mol Sci.  https://doi.org/10.3390/ijms19020469 Google Scholar
  17. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil, vol 347, 2nd edn. California Agricultural Experiment Station, Circular, California, pp 1–32Google Scholar
  18. Hubbart JA, Kellner E, Hooper LW, Zeiger S (2017) Quantifying loading, toxic concentrations, and systemic persistence of chloride in a contemporary mixed-land-use watershed using an experimental watershed approach. Sci Total Environ 581:822–832.  https://doi.org/10.1016/j.scitotenv.2017.01.019 CrossRefGoogle Scholar
  19. Ibrahim W, Qiu C, Zhang C, Cao F, Shu Z, Wu F (2018a) Comparative physiological analysis in the tolerance to salinity and drought individual and combination in two cotton genotypes with contrasting salt tolerance. Physiol Plant 165:155–168.  https://doi.org/10.1111/ppl.12791 CrossRefGoogle Scholar
  20. Ibrahim MEH, Zhu X, Zhou G, Ali AYA, Ahmad I, Farah GA (2018b) Nitrogen fertilizer alleviated negative impacts of NaCl on some physiological parameters of wheat. Pak J Bot 50:2097–2104Google Scholar
  21. Kaya O, Kose C, Gecim T (2018) An exothermic process involved in the late spring frost injury to flower buds of some apricot cultivars (Prunus armenica L.). Sci Hortic 241:322–328.  https://doi.org/10.1016/j.scienta.2018.07.019 CrossRefGoogle Scholar
  22. Kozlowski TT, Pallardy SG (2002) Acclimation and adaptive responses of woody plants to environmental stresses. Bot Rev 68:270–334.  https://doi.org/10.1663/0006-8101(2002)068%5B0270:aaarow%5D2.0.co;2 CrossRefGoogle Scholar
  23. Lang J, Shi M, Ran L (2016) Effect of repeated freeze-thaw on inhibition of Bacillus subtilis strain St-zn-34 to Alternaria alternate. Acta Microbiol Sin 56:1616–1625.  https://doi.org/10.13343/j.cnki.wsxb.20150622 (in Chinese).Google Scholar
  24. Li L, Li J, Shao H, Dong Y (2018) Effects of low-vacuum helium cold plasma treatment on seed germination, plant growth and yield of oilseed rape. Plasma Sci Technol.  https://doi.org/10.1088/2058-6272/aac3d0 Google Scholar
  25. Lindlof A, Chawade A, Sikora P, Olsson O (2015) Comparative transcriptomics of Sijung and Jumli Marshi rice during early chilling stress imply multiple protective mechanisms. PLoS ONE.  https://doi.org/10.1371/journal.pone.0125385 Google Scholar
  26. Moles TM, Mariotti L, Federico De Pedro L, Guglielminetti L, Picciarelli P, Scartazza A (2018) Drought induced changes of leaf-to-root relationships in two tomato genotypes. Plant Physiol Biochem 128:24–31.  https://doi.org/10.1016/j.plaphy.2018.05.008 CrossRefGoogle Scholar
  27. Nie X, Lei J, Chen S, Zhang Y, Zhang C, Hong W (2018) Physiological, proteomic, and gene expression analysis of turbot (Scophthalmus maximus) in response to cold acclimation. Aquaculture 495:281–287.  https://doi.org/10.1016/j.aquaculture.2018.05.054 CrossRefGoogle Scholar
  28. Palliyath S, Puthur JT (2018) The modulation of various physiochemical changes in Bruguiera cylindrica (L.) Blume affected by high concentrations of NaCl. Acta Physiol Plant.  https://doi.org/10.1007/s11738-018-2735-3 Google Scholar
  29. Pedersen JBT, Bartholdy J (2007) Exposed salt marsh morphodynamics: an example from the Danish Wadden Sea. Geomorphology 90:115–125.  https://doi.org/10.1016/j.geomorph.2007.01.012 CrossRefGoogle Scholar
  30. Sahin U, Ekinci M, Ors S, Turan M, Yildiz S, Yildirim E (2018) Effects of individual and combined effects of salinity and drought on physiological, nutritional and biochemical properties of cabbage (Brassica oleracea var. capitata). Sci Hortic 240:196–204.  https://doi.org/10.1016/j.scienta.2018.06.016 CrossRefGoogle Scholar
  31. Schoukens I, Cavezza F, Cerezo J, Vandenberghe V, Gudla VC, Ambat R (2018) Influence of de-icing salt chemistry on the corrosion behavior of AA6016. Mater Corros 69, 881–887.  https://doi.org/10.1002/maco.201709907 CrossRefGoogle Scholar
  32. Solhaug KA, Chowdhury DP, Gauslaa Y (2018) Short- and long-term freezing effects in a coastal (Lobaria virens) versus a widespread lichen (L. pulmonaria). Cryobiology 82:124–129.  https://doi.org/10.1016/j.cryobiol.2018.03.007 CrossRefGoogle Scholar
  33. Song X, Wang S, Jiang Y (2017) Genotypic variations in plant growth and nutritional elements of perennial ryegrass accessions under salinity stress. J Am Soc Hortic Sci 142:476–483.  https://doi.org/10.21273/jashs04258-17 CrossRefGoogle Scholar
  34. Wang X (2010) Principle and technology of plant physiological and biochemical experiments, 2nd edn. Higher Education, Beijing, pp 190–281 (in Chinese)Google Scholar
  35. Wang S, Huang H, Tang J, Wang R, Wang X (2012) Effects of low temperature stress on the content of malondialdehyde in rape seedlings. Hubei Agric Sci 51(20):4467–4469.  https://doi.org/10.3969/j.issn.0439-8114.2012.20.006 (in Chinese).Google Scholar
  36. Wang H, Gong M, Xin H, Tang L, Dai D, Gao Y, Liu C (2018a) Effects of chilling stress on the accumulation of soluble sugars and their key enzymes in Jatropha curcas seedlings. Physiol Mol Biol Plants 24:857–865.  https://doi.org/10.1007/s12298-018-0568-6 CrossRefGoogle Scholar
  37. Wang J, Anderson CWN, Xing Y, Fan Y, Xia J, Shaheen SM, Rinklebe J, Feng X (2018b) Thiosulphate-induced phytoextraction of mercury in Brassica juncea: spectroscopic investigations to define a mechanism for Hg uptake. Environ Pollut 242:986–993.  https://doi.org/10.1016/j.envpol.2018.07.065 CrossRefGoogle Scholar
  38. Xing K, Li T, Liu Y, Zhang J, Zhang Y, Shen X, Li X, Miao X, Feng Z, Peng X, Li Z, Qin S (2018) Antifungal and eliciting properties of chitosan against Ceratocystis fimbriata in sweet potato. Food Chem 268:188–195.  https://doi.org/10.1016/j.foodchem.2018.06.088 CrossRefGoogle Scholar
  39. Yang Z (2014) Cold stress and temperature rise on the physioligical and biochemical indexes sandalwood impact. Dissertation, Fujian Forestry University (in Chinese)Google Scholar
  40. Yao P, Sun Z, Li C, Zhao X, Li M, Deng R, Huang Y, Zhao H, Chen H, Wu Q (2018) Overexpression of Fagopyrum tataricum FtbHLH2 enhances tolerance to cold stress in transgenic Arabidopsis. Plant Physiol Biochemistry 125:85–94.  https://doi.org/10.1016/j.plaphy.2018.01.028 CrossRefGoogle Scholar
  41. Zhang Z (2000) Plant physiological experiment. Higher Education, Beijing (in Chinese)Google Scholar
  42. Zhang X, Feng L, Yang T, Xu Z, Hu L (2015) Effects of chilling stress on physiological characteristics of rapeseed seedlings in winte. Plant Physiol J 51:737–746.  https://doi.org/10.13592/j.cnki.ppj.2014.0411. (in Chinese).Google Scholar
  43. Zhao R, Guo F, An L, Chen Y, Guo A, Cao S (2016) Growth and physiological responses of Astragalus membranaceus var. mongolicus seedlings to salt stress. J Northwest For Univ 31(03):23–29.  https://doi.org/10.3969/j.issn.1001-7461.2016.03.004 (in Chinese).Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of New Energy and EnvironmentJilin UniversityChangchunChina
  2. 2.College of Animal ScienceJilin UniversityChangchunChina
  3. 3.Jilin UniversityChangchunChina

Personalised recommendations