Journal of Plant Growth Regulation

, Volume 38, Issue 4, pp 1206–1214 | Cite as

Study on the Physiological Mechanism of Early Flowering and Low Male Fertility of Limonium bicolor Mutant vrl15

  • Bingying Leng
  • Pengfei Zhao
  • Xinxiu Dong
  • Fang YuanEmail author
  • Baoshan WangEmail author


As biennial recretohalophytes, Limonium bicolor plants need 2 years to complete their life cycle. A growth habit mutant Vernalization Requirement Loss 15 (vrl15) was obtained by ion implantation. However, the biological characteristics of the mutant were unclear. In the current study, the related traits of vrl15 and some possible reasons for these traits were examined. Compared with wild type (WT), vrl15 can bolt and flower in approximately four months without vernalization. Moreover, vrl15 needed much less time to bolting and flowering than wild-type L. bicolor under different vernalization treatments. After 20 days’ vernalization, bolting vrl15 plants had 24 rosette leaves and bolting WT had 31 rosette leaves. Moreover, the pollen number per anther, the proportion of active pollen, the seed setting rate and the 1000 seed weight of vrl15 were all lower than those of WT. The soluble sugar content and soluble protein content in leaves of the vrl15 were much higher than those of WT sowed at the same time. In addition, the GA content in the leaves of bolting vrl15 was higher than that of the non-bolting WT sowed at the same time and non-bolting vrl15, whereas the contents of ABA and BR were much lower than that of the non-bolting WT. These results indicate that to some extent the increase of GA and decrease of ABA and BR content may be involved in the growth habit and male fertility alteration of mutant vrl15 of L. bicolor.


Limonium bicolor Growth habit Vernalization Requirement Loss 15 Vernalization Endogenous hormone 



This work was supported by the NSFC (National Natural Science Research Foundation of China, Project Nos. 31570251; 31600200; 31770288), Shandong Province Key Research and Development Plan (2017CXGC0313), the Natural Science Research Foundation of Shandong Province (ZR2014CZ002; ZR2017MC003), and the Higher Educational Science and Technology Program of Shandong Province (J17KA136).

Author Contributions

BSW and BYL designed the experiment. BYL, PFZ and XXD performed the experiment. BYL, PFZ and FY finished the manuscript. All authors checked and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors have declared that no competing interests exist.


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Authors and Affiliations

  1. 1.Shandong Provincial Key Laboratory of Plant Stress, College of Life SciencesShandong Normal UniversityJinanPeople’s Republic of China

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