Effects of Modifiers on the Growth, Photosynthesis, and Antioxidant Enzymes of Cotton Under Cadmium Toxicity

  • MengJie An
  • HaiJiang Wang
  • Hua Fan
  • J. A. Ippolito
  • Chunmei Meng
  • Yulian E.
  • Yingbin Li
  • Kaiyong WangEmail author
  • Changzhou WeiEmail author


The effects of four liquid modifiers (organic–inorganic composite modifier, inorganic polymer compound modifier, polyacrylate compound modifier, and organic polymer compound modifier) on plant growth, cadmium (Cd) content, photosynthetic parameters and antioxidant enzymes were studied in cotton (Xinluzao) under Cd stress (5 mg kg−1) in a barrel experiment. The results showed that the Cd treatment of soil increased Cd content in cotton and reduced plant height, net photosynthesis rate (Pn), chlorophyll fluorescence parameters, antioxidant enzyme activity, and biomass. However, the application of liquid modifiers alleviated Cd stress, increased plant biomass, and decreased Cd content in plant organs as well as Cd transport coefficients of the stem and cottonseed. At the same time, there was an increase in gas exchange, photosynthetic pigment content, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activity in cotton leaves, whereas malondialdehyde content (MDA) decreased. These results suggest a positive role of liquid modifiers in alleviating Cd stress in cotton, which was due to (1) significant reduction in Cd uptake of roots and Cd-transportation to leaves and (2) improvement in the antioxidant activity, which regulated the oxidants to a level under control, minimizing the oxidative damage in cotton.


Cadmium (Cd) Liquid modifiers Transport coefficient Photosynthetic fluorescence Antioxidant 



This research was supported by the International Science & Technology Cooperation Program of China (2015DFA11660), the National Key Research and Development Program of China (2016YFC0501406), the National Natural Science Foundation of China (31560169), and the International Science & Technology Cooperation Promoting Plan of Shihezi University (GJHZ201706), the International Science & Technology Cooperation Program of Shihezi University (GJHZ201802).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Ahammed GJ, Choudhary SP, Chen SC, Xia XJ, Shi K, Zhou YH, Yu JQ (2013) Role of brassinosteroids in alleviation of phenanthrene-cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomato. J Exp Bot 64(1):199–213CrossRefGoogle Scholar
  2. Ali B, Huang CR, Qi ZY, Ali S, Daud MK, Geng XX, Liu HB, Zhou WJ (2013a) 5-Aminolevulinic acid ameliorates cadmium-induced morphological, biochemical, and ultrastructural changes in seedlings of oilseed rape. Environ Sci Pollut Res 20(10):7256–7267CrossRefGoogle Scholar
  3. Ali B, Wang B, Ali S, Ghani MA, Hayat MT, Yang C, Xu L, Zhou WJ (2013b) 5-Aminolevulinic acid ameliorates the growth, photosynthetic gas exchange capacity, and ultrastructural changes under cadmium stress in Brassica napus L. J Plant Growth Regul 32(3):604–614CrossRefGoogle Scholar
  4. Ali B, Qian P, Jin R, Ali S, Khan M, Aziz R, Tian T, Zhou W (2014) Physiological and ultra-structural changes in Brassica napus seedlings induced by cadmium stress. Biol Plant 58(1):131–138CrossRefGoogle Scholar
  5. Anwaar SA, Ali S, Ishaque W, Farid M, Farooq MA, Najeeb U, Abbas F, Sharif M (2014) Silicon (Si) alleviates cotton (Gossypium hirsutum L.) fromzinc (Zn) toxicity stress by limiting Zn uptake and oxidative damage. Environ Sci Pollut Res 22:3441–3450CrossRefGoogle Scholar
  6. Arif SW, Inayatullah T, Syed SA, Riyaz AD, Shaziya N (2017) Efficacy of 24-epibrassinolide in improving the nitrogen metabolism and antioxidant system in chickpea cultivars under cadmium and/or NaCl stress. Sci Hortic 225:48–55CrossRefGoogle Scholar
  7. Bao SD (2000) Soil agrochemical analysis, 3rd edition. China Agriculture Press, BeijingGoogle Scholar
  8. Bloomfield KJ, Farquhar GD, Lloyd J (2014) Photosynthesis–nitrogen relationships in tropical forest tree species as affected by soil phosphorus availability: a controlled environment study. Funct Plant Biol 41(8):820CrossRefGoogle Scholar
  9. Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98(4):1222–1227CrossRefGoogle Scholar
  10. Chu J, Zhu F, Chen X, Liang H, Wang R, Wang X, Huang X (2018) Effects of cadmium on photosynthesis of Schima superba young plant detected by chlorophyll fluorescence. Environ Sci Pollut Res 25(11):10679–10687CrossRefGoogle Scholar
  11. Daud MK, Quiling H, Lie M, Ali B, Zhu SJ (2015) Ultrastructural, metabolic and proteomic changes in leaves of upland cotton in response to cadmium stress. Chemosphere 120:309–320CrossRefGoogle Scholar
  12. Daud MK, Mei L, Azizullah A, Dawood M, Ali I, Mahmood Q, Ullah W, Jamil M, Zhu SJ (2016) Leaf-based physiological, metabolic, and ultrastructural changes in cultivated cotton cultivars under cadmium stress mediated by glutathione. Environ Sci Pollut Res 23(15):1–14CrossRefGoogle Scholar
  13. Duan G, Zhang H, Shen Y, Li G, Wang H, Cheng WD (2016) Mitigation of heavy metal accumulation in rice grain with silicon in animal manure fertilized field. Environ Eng Manage J 15(10):2223–2229CrossRefGoogle Scholar
  14. Fang ZP, Liao M, Zhang N, Lv T, Huang XH (2017) Effect of sepiolite application on the migration and redistribution of Pb and Cd in soil rice system in soil with Pb and Cd combined contamination. Environ Sci 38(7):3028–3035Google Scholar
  15. Farooq MA, Ali S, Hameed A, Bharwana SA, Rizwan M, Ishaque W, Farid M, Mahmood K, Iqbal Z (2016) Cadmium stress in cotton seedlings: physiological, photosynthesis and oxidative damages alleviated by glycinebetaine. S Afr J Bot 104:61–68CrossRefGoogle Scholar
  16. Gallego SM, Pena LB, Barcia RA, Azpilicueta CE, Iannone MF, Rosales EP, Zawoznik MS, Groppa MD, Benavides MP (2012) Unraveling cadmium toxicity and tolerance in plants: insight into regulatory mechanisms. Environ Exp Bot 83:33–46CrossRefGoogle Scholar
  17. Hasan SA, Hayat S, Ahmad A (2011) Brassinosteroids protect photosynthetic machinery against the cadmium induced oxidative stress in two tomato cultivars. Chemosphere 84:1446–1451CrossRefGoogle Scholar
  18. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I—kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198CrossRefGoogle Scholar
  19. Jia L, Liu Z, Chen W, Ye Y, Yu S, He XY (2015) Hormesis effects induced by cadmium on growth and photosynthetic performance in a hyperaccumulator, Lonicera japonica, Thunb. J Plant Growth Regul 34(1):13–21CrossRefGoogle Scholar
  20. Jian MF, Wang SC, Yu HP, Li LY, Jian MF, Yu GJ (2016) Influence of Cd2+ or Cu2+ stress on the growth and photosynthetic fluorescence characteristics of Hydrilla verticillata. Acta Ecol Sin 36(6):1719–1727Google Scholar
  21. Li S, Yang W, Yang T, Chen Y, Ni WZ (2015) Effects of cadmium stress on leaf chlorophyll fluorescence and photosynthesis of Elsholtzia argyi: a cadmium accumulating plant. Int J Phytorem 17(1):85–92CrossRefGoogle Scholar
  22. Li L, Ai S, Li Y, Wang YH, Tang MD (2017) Exogenous silicon mediates alleviation of cadmium stress by promoting photosynthetic activity and activities of antioxidative enzymes in rice. J Plant Growth Regul 37(2):1–10Google Scholar
  23. Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148(1):350–382CrossRefGoogle Scholar
  24. Ling L, Chen JH, He QL, Muhammad KD, Zhu SJ (2012) Characterization of physiological traits, yield and fiber quality in three upland cotton cultivars grown under cadmium stress. Aust J Crop Sci 6(11):1527–1533Google Scholar
  25. Liu X, Mak M, Babla M, Wang F, Chen G, Veljanoski F, Wang G, Shabala S, Zhou M, Chen Z (2014) Linking stomatal traits and expression of slow anion channel genes HvSLAH1 and HvSLAC1with grain yield for increasing salinity tolerance in barley. Front Plant Sci 5:1–12Google Scholar
  26. Ozfidankonakci C, Yildiztugay E, Bahtiyar M, Kucukoduk M (2018) The humic acid-induced changes in the water status, chlorophyll fluorescence and antioxidant defense systems of wheat leaves with cadmium stress. Ecotoxicol Environ Saf 155:66–75CrossRefGoogle Scholar
  27. Paoletti F, Aldinucci D, Mocali A, Caparrini A (1986) A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem 154(2):536–541CrossRefGoogle Scholar
  28. Qin X, Nie Z, Liu H, Zhao P, Qin S, Shi Z (2018) Influence of selenium on root morphology and photosynthetic characteristics of winter wheat under cadmium stress. Environ Exp Bot 150:232–239CrossRefGoogle Scholar
  29. Saidi I, Ayouni M, Dhieb A, Chtourou Y, Chaïbi W, Djebali W (2013) Oxidative damages induced by short-term exposure to cadmium in bean plants: protective role of salicylic acid. S Afr J Bot 85:32–38CrossRefGoogle Scholar
  30. Seleiman MF, Kheir AM (2018) Saline soil properties, quality and productivity of wheat grown with bagasse ash and thiourea in different climatic zones. Chemosphere 193, 538e546CrossRefGoogle Scholar
  31. Wei X, Wei YX, Guo D, Sun B, Wang XD, Liu C (2015) Effects of different breaking dormancy ways on the photosynthetic characteristics and activities of protective enzymes of ‘misty’ blueberry leaves. Sci Agric Sin 48(22):4517–4528Google Scholar
  32. Xie XF, Fang ZP, Liao M, Huang Y, Huang XH (2018) Potential to ensure the safe production of heavy cadmium polluted rice fields by combination of rice variety with low cadmium accumulation, humic acid and sepiolite. Environ Sci 09:1–23CrossRefGoogle Scholar
  33. Yan JP, Ding XD, Cui L, Zhang L (2018) Effects of several modifiers and their combined application on cadmium forms and physicochemical properties of soil. J Agro-Environ Sci 37(9):1842–1849Google Scholar
  34. Yang Y, ZHou K, Xu WH, Jian L, Wang CL, Xiong SJ, Xie WW, Chen R, Xiong ZT, Wang ZY, Xie DT (2015) Effect of exogenous iron on photosynthesis, quality, and accumulation of cadmium in different varieties of tomato. J Plant Nutr Fertil 21(4):1006–1015Google Scholar
  35. Zaid A, Mohammad F (2018) Methyl jasmonate and nitrogen interact to alleviate cadmium stress in mentha arvensis by regulating physio-biochemical damages and ros detoxification. J Plant Growth Regul 37:1435–8107CrossRefGoogle Scholar
  36. Zhang Z, Rengel Z, Meney K, Pantelic L, Tomanovic R (2011) Polynuclear aromatic hydrocarbons (pahs) mediate cadmium toxicity to an emergent wetland species. J Hazard Mater 189(1):119–126CrossRefGoogle Scholar
  37. Zhao FJ, Jiang RF, Dunham SJ, McGrath SP (2006) Cadmium uptake, translocation and tolerance in the hyperaccumulator Arabidopsis halleri. New Phytol J 172:646–654CrossRefGoogle Scholar
  38. Zhou WJ, Leul M (1999) Uniconazole-induced tolerance of rape plants to heat stress in relation to changes in hormonal levels, enzyme activities and lipid peroxidation. Plant Growth Regul 27:99–104CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • MengJie An
    • 1
  • HaiJiang Wang
    • 1
  • Hua Fan
    • 1
  • J. A. Ippolito
    • 1
    • 2
  • Chunmei Meng
    • 1
  • Yulian E.
    • 1
  • Yingbin Li
    • 1
  • Kaiyong Wang
    • 1
    Email author
  • Changzhou Wei
    • 1
    Email author
  1. 1.Agriculture CollegeShihezi UniversityXinjiangChina
  2. 2.Department of Soil and Crop SciencesColorado State UniversityFort CollinsUSA

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