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Genome-Wide Identification of Trihelix Genes in Moso Bamboo (Phyllostachys edulis) and Their Expression in Response to Abiotic Stress

  • Hongyan Gao
  • Rong Huang
  • Jun Liu
  • Zhimin Gao
  • Hansheng Zhao
  • Xueping LiEmail author
Article
  • 24 Downloads

Abstract

Trihelix proteins, which are a plant-specific family of transcription factors (TFs), play important roles in plant growth and development, as well as in the plant response to biotic and abiotic stressors. The DNA-binding domain of trihelix proteins is a tryptophan-enriched tandem repeat, forming a helix–loop–helix–loop–helix structure. Here, we identified and characterized 24 full-length trihelix genes from Phyllostachys edulis and classified these genes into 6 previously described subfamilies. Within a given subfamily, the trihelix genes shared similar conserved motifs and structures. Most of the promoters of the trihelix genes in P. edulis contained stress-related cis-elements. Our tissue-specific and gene expression analysis using quantitative real-time PCR indicated that the trihelix genes were expressed primarily in the stems, followed by leaves and roots, and were regulated by cold, drought, and salt stresses. This study delivers novel insights into the phylogenetic relationships and functions of the trihelix TFs of P. edulis. Our results provide a framework for further functional studies investigating the diverse roles of trihelix genes in other bamboo species.

Keywords

Trihelix transcription factor Phyllostachys edulis Stress qRT-PCR 

Notes

Acknowledgements

Sincere thanks to the Members of the Key Laboratory of Bamboo and Rattan Science and Technology for their help in this study.

Compliance with Ethical Standards

Conflict of interest

The authors declare no competing financial interests. All the authors agreed to the submission of this article.

Supplementary material

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Supplementary material 1 (DOCX 16 KB)
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344_2019_9918_MOESM3_ESM.xlsx (19 kb)
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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hongyan Gao
    • 1
  • Rong Huang
    • 1
  • Jun Liu
    • 1
  • Zhimin Gao
    • 1
  • Hansheng Zhao
    • 1
  • Xueping Li
    • 1
    Email author
  1. 1.International Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and TechnologyNational Forestry and Grassland AdministrationBeijingPeople’s Republic of China

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