Different Responses of Capsicum annuum L. Root and Shoot to Salt Stress with Pseudomonas putida Rs-198 Inoculation
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The aim of this work was to compare the different responses of the root and the shoot to salt stress with or without growth-promoting bacteria Pseudomonas putida Rs-198 application. The results revealed that lower salt stress did not show bad effects on plant growth although osmotic tolerance indictors were observed. The results showed that proline content increased by 7.75% and 20.20% in shoot and 323.73% and 449.38% in root under 150 mM and 300 mM salt treatment, respectively. Increasing salt stress also led to a reduction of peroxides enzyme (POD) activity by 16.50% and 15.49% in the shoot and 46.03% and 39.36% in the root under above salt treatments. More importantly, the changes observed in pepper roots were more pronounced than those in pepper shoots except for total soluble sugar and protein. In addition, inoculation of Pseudomonas putida Rs-198 could relieve the changes of these indicators. Further, these changes were more pronounced in non-inoculated control plants than in inoculated plants. Especially, Pseudomonas putida Rs-198-inoculated plants exhibited the greatest increase in polyphenol oxidase activity by 78.06% in shoot and POD activity by 25.22% in root compared with the non-inoculated plants at 150 mM NaCl stress. To the best of our knowledge, these results are the first reported that will more effectively explain the phenomena relationship between plant, salt stress, and plant growth-promoting bacteria.
Physiological changes induced by Pseudomonas putida Rs-198 interaction in Capsicum annuum L. under salt stress.
KeywordsPseudomonas putida Rs-198 Capsicum annuum L. Salinity stress Osmotic adjustment Antioxidative enzyme
We would like to thank Mike DiLegge, Vincent Lopez form Center of Rhizosphere Biology, Colorado State University for the review and language edit.
This work was financially supported by Natural Science Foundation of China (31260022, 21566035), Postgraduate Education Reform and Innovation Program of Autonomous Regions (XJGR12016031), Scientific Research Foundation for Changjiang Scholars of Shihezi University (CJXZ201501). Yanhui He was supported by China Scholarship Council (No. 201709505007).
Compliance with Ethical Standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- Ali S, Rizwan M, Qayyum MF, Ok YS, Ibrahim M, Riaz M, Arif MS, Hafeez F, Al-Wabel MI, Shahzad AN (2017) Biochar soil amendment on alleviation of drought and salt stress in plants: a critical review. Environ Sci Pollut Res Int 24:12700–12712. https://doi.org/10.1007/s11356-017-8904-x CrossRefGoogle Scholar
- Fan JW, Du YL, Wang BR, Turner NC, Wang T, Abbott LK, Stefanova K, Siddique KHM, Li FM (2016) Forage yield, soil water depletion, shoot nitrogen and phosphorus uptake and concentration, of young and old stands of alfalfa in response to nitrogen and phosphorus fertilisation in a semiarid environment. Field Crop Res 198:247–257. https://doi.org/10.1016/j.fcr.2016.08.014 CrossRefGoogle Scholar
- Guo P, Song B, Xu L, Zheng Y, Li R, Zhang H, Huang H (2011) An improved spectrophotometric method for determination of betaine content in plants. J Guangzhou Univ (Nat Sci Ed) 10:32–36Google Scholar
- Li X, Geng X, Xie R, Fu L, Jiang J, Gao L, Sun J (2016) The endophytic bacteria isolated from elephant grass (Pennisetum purpureum Schumach) promote plant growth and enhance salt tolerance of Hybrid Pennisetum. Biotechnol Biofuel 9:190. https://doi.org/10.1186/s13068-13016-10592-13060 CrossRefGoogle Scholar
- Nautiyal CS, Srivastava S, Chauhan PS, Seem K, Mishra A, Sopory SK (2013) Plant growth-promoting bacteria Bacillus amyloliquefaciens NBRISN13 modulates gene expression profile of leaf and rhizosphere community in rice during salt stress. Plant Physiol Biochem 66:1–9. https://doi.org/10.1016/j.plaphy.2013.01.020 CrossRefGoogle Scholar
- Olaiya C, Poloamina L (2013) Changes in the contents of carotenoid, chlorophyll and antioxidant enzymes in the leaf tissues of Pepper (Capsicum annuum L.) following exogenous application of bioregulators. Nat Sci 11:9–13Google Scholar
- Shalaby T, Bayoumi Y, Alshaal T, Elhawat N, Sztrik A, El-Ramady H (2017) Selenium fortification induces growth, antioxidant activity, yield and nutritional quality of lettuce in salt-affected soil using foliar and soil applications. Plant Soil 421:245–258. https://doi.org/10.1007/s11104-017-3458-8 CrossRefGoogle Scholar
- Troll W, Lindsley J (1955) A photometric method for the determination of proline. J Biol Chem 215:655–660Google Scholar
- Yang Y, Tang M, Sulpice R, Chen H, Tian S, Ban Y (2014) Arbuscular mycorrhizal fungi alter fractal dimension characteristics of Robinia pseudoacacia L. seedlings through regulating plant growth, leaf water status, photosynthesis, and nutrient concentration under drought stress. J Plant Growth Regul 33:612–625CrossRefGoogle Scholar