Effects of Exogenous Spermidine and Elevated CO2 on Physiological and Biochemical Changes in Tomato Plants Under Iso-osmotic Salt Stress
- 78 Downloads
Carbon dioxide (CO2) enrichment is used to boost crop yield in greenhouse vegetable production that often exposes vegetables to the simultaneous occurrence of elevated CO2 and salinity due to frequent irrigation and fertilization in facility horticulture. The beneficial effects of exogenous spermidine (Spd, a kind of polyamine) on plant growth and development under salt stress have been widely reported; however, little information is available on the effects of Spd on the combined treatment of CO2 enrichment and iso-osmotic salt stress. In this work, the effects of exogenous Spd (0.25 mM) on plant growth, chlorophyll content, water status, osmotic adjustment, and the antioxidant system were investigated under CO2 enrichment (800 ppm) and iso-osmotic salt stress [150 mmol/L NaCl and 100 mmol/L Ca(NO3)2] in tomato (Solanum lycopersicum L.). The results showed that iso-osmotic salt stress significantly decreased fresh and dry weights of plants, relative water content, total root length, total root surface area, total root volume, and average root diameter in tomato plants. However, either elevated CO2 or exogenous Spd both attenuated iso-osmotic salt stress-induced reductions in growth parameters and the combined treatment of elevated CO2 and Spd showed a more profound effect, leading to the enhanced tolerance to salt stress in tomato plants. Elevated CO2 and/or exogenous Spd-induced alleviation of iso-osmotic salt stress was associated with efficient osmotic adjustment and antioxidant defense, which minimized the salt stress-induced oxidative stress as well. Therefore, Spd application can be advocated to mitigate secondary salinization in protected vegetable production where elevated CO2 is simultaneously used to boost crop yield.
KeywordsCO2 enrichment Iso-osmotic salt stress Polyamines Secondary salinization Spermidine Tomato
This work was supported by the National Natural Science Foundation of China (31501807, 31501750, 31471867), China Postdoctoral Science Foundation (2018M631769), Henan University of Science and Technology Research Start-up Fund for New Faculty (13480058), Henan Natural Science Foundation (182300410046), Science and Technology Innovation Talents Support Plan of Henan Province (19HASTIT009), Programs for Science and Technology Development of Henan province (172102410050) and the Key Laboratory of Horticultural Crop Growth and Quality Control in Protected Environment of Luoyang City.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
- Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction annual. Rev Plant Biol 55:373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701 CrossRefGoogle Scholar
- Huang SW, Gao W, Tang JW, Chun-Hua LI (2016) Total salt content and ion composition in tillage layer of soils in the main vegetable production regions of China. J Plant Nutr Fertil 22:965Google Scholar
- Sgherri C, Perez-Lopez U, Micaelli F, Miranda-Apodaca J, Mena-Petite A, Munoz-Rueda A, Quartacci MF (2017) Elevated CO2 and salinity are responsible for phenolics-enrichment in two differently pigmented lettuces. Plant Physiol Biochem. 115:269–278. https://doi.org/10.1016/j.plaphy.2017.04.006 CrossRefPubMedGoogle Scholar
- Tong H, Zhang ZX, Bin LI, Wang JW, Guo SR (2012) Effects of Iso-osmotic Ca(NO3)2 and NaCl stress on chloroplast ultra-structure and photosynthesis in cucumber leaves. China Veg 18:160–165Google Scholar
- Zandalinas SI, Mittler R (2017) ROS-induced ROS release in plant and animal cells. Free Rad Biol Med. https://doi.org/10.1016/j.freeradbiomed.2017.11.028 CrossRefPubMedGoogle Scholar
- Zhang Y et al (2013) Beneficial role of exogenous spermidine on nitrogen metabolism in tomato seedlings exposed to saline-alkaline stress. J Am Soc Hortic Sci 138:38–49Google Scholar