Advertisement

Journal of Plant Growth Regulation

, Volume 37, Issue 4, pp 1222–1234 | Cite as

Effects of Exogenous Spermidine and Elevated CO2 on Physiological and Biochemical Changes in Tomato Plants Under Iso-osmotic Salt Stress

  • Zhang Yi
  • Shuo Li
  • Ying Liang
  • Hailiang Zhao
  • Leiping Hou
  • Shi Yu
  • Golam Jalal Ahammed
Article
  • 78 Downloads

Abstract

Carbon dioxide (CO2) enrichment is used to boost crop yield in greenhouse vegetable production that often exposes vegetables to the simultaneous occurrence of elevated CO2 and salinity due to frequent irrigation and fertilization in facility horticulture. The beneficial effects of exogenous spermidine (Spd, a kind of polyamine) on plant growth and development under salt stress have been widely reported; however, little information is available on the effects of Spd on the combined treatment of CO2 enrichment and iso-osmotic salt stress. In this work, the effects of exogenous Spd (0.25 mM) on plant growth, chlorophyll content, water status, osmotic adjustment, and the antioxidant system were investigated under CO2 enrichment (800 ppm) and iso-osmotic salt stress [150 mmol/L NaCl and 100 mmol/L Ca(NO3)2] in tomato (Solanum lycopersicum L.). The results showed that iso-osmotic salt stress significantly decreased fresh and dry weights of plants, relative water content, total root length, total root surface area, total root volume, and average root diameter in tomato plants. However, either elevated CO2 or exogenous Spd both attenuated iso-osmotic salt stress-induced reductions in growth parameters and the combined treatment of elevated CO2 and Spd showed a more profound effect, leading to the enhanced tolerance to salt stress in tomato plants. Elevated CO2 and/or exogenous Spd-induced alleviation of iso-osmotic salt stress was associated with efficient osmotic adjustment and antioxidant defense, which minimized the salt stress-induced oxidative stress as well. Therefore, Spd application can be advocated to mitigate secondary salinization in protected vegetable production where elevated CO2 is simultaneously used to boost crop yield.

Keywords

CO2 enrichment Iso-osmotic salt stress Polyamines Secondary salinization Spermidine Tomato 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31501807, 31501750, 31471867), China Postdoctoral Science Foundation (2018M631769), Henan University of Science and Technology Research Start-up Fund for New Faculty (13480058), Henan Natural Science Foundation (182300410046), Science and Technology Innovation Talents Support Plan of Henan Province (19HASTIT009), Programs for Science and Technology Development of Henan province (172102410050) and the Key Laboratory of Horticultural Crop Growth and Quality Control in Protected Environment of Luoyang City.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Alcazar R et al (2006) Involvement of polyamines in plant response to abiotic stress. Biotechnol Lett 28:1867–1876.  https://doi.org/10.1007/s10529-006-9179-3 CrossRefPubMedGoogle Scholar
  2. Alsaeedi A, El-Ramady H, Alshaal T, El-Garawani M, Elhawat N, Al-Otaibi A (2018) Exogenous nanosilica improves germination and growth of cucumber by maintaining K+/Na+ ratio under elevated Na+ stress. Plant Physiol Biochem.  https://doi.org/10.1016/j.plaphy.2018.02.006 CrossRefPubMedGoogle Scholar
  3. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction annual. Rev Plant Biol 55:373–399.  https://doi.org/10.1146/annurev.arplant.55.031903.141701 CrossRefGoogle Scholar
  4. Bailly C, Benamar A, Corbineau F, Come D (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiol Plant 97:104–110CrossRefGoogle Scholar
  5. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207.  https://doi.org/10.1007/bf00018060 CrossRefGoogle Scholar
  6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  7. Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98:1222–1227CrossRefPubMedGoogle Scholar
  8. Cassia R, Nocioni M, Correa-Aragunde N, Lamattina L (2018) Climate change and the impact of greenhouse gasses: CO2 and NO, friends and foes of plant oxidative stress. Front Plant Sci 9:273.  https://doi.org/10.3389/fpls.2018.00273 CrossRefPubMedGoogle Scholar
  9. Du CX, Fan HF, Guo SR, Tezuka T, Li J (2010) Proteomic analysis of cucumber seedling roots subjected to. Salt Stress Phytochem 71:1450–1459.  https://doi.org/10.1016/j.phytochem.2010.05.020 CrossRefGoogle Scholar
  10. Geissler N, Hussin S, Koyro HW (2009) Elevated atmospheric CO2 concentration ameliorates effects of NaCl salinity on photosynthesis and leaf structure of Aster tripolium L.. J Exp Botany 60:137–151.  https://doi.org/10.1093/jxb/ern271 CrossRefGoogle Scholar
  11. Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59:309–314CrossRefPubMedGoogle Scholar
  12. Hossain MS, Alam MU, Rahman A, Hasanuzzaman M, Nahar K, Al Mahmud J, Fujita M (2017) Use of iso-osmotic solution to understand salt stress responses in lentil (Lens culinaris Medik.). S Afr J Bot 113:346–354.  https://doi.org/10.1016/j.sajb.2017.09.007 CrossRefGoogle Scholar
  13. Huang SW, Gao W, Tang JW, Chun-Hua LI (2016) Total salt content and ion composition in tillage layer of soils in the main vegetable production regions of China. J Plant Nutr Fertil 22:965Google Scholar
  14. Lechno S, Zamski E, Tel-Or E (1997) Salt stress-induced responses in cucumber plants. J Plant Physiol 150:206–211.  https://doi.org/10.1016/s0176-1617(97)80204-0 CrossRefGoogle Scholar
  15. Li X et al (2015a) Carbon dioxide enrichment alleviates heat stress by improving cellular redox homeostasis through an ABA-independent process in tomato plants. Plant Biol 17:81–89.  https://doi.org/10.1111/plb.12211 CrossRefPubMedGoogle Scholar
  16. Li XJ et al (2015b) DWARF overexpression induces alteration in phytohormone homeostasis, development, architecture and carotenoid accumulation in tomato. Plant Biotechnol J  https://doi.org/10.1111/pbi.12474 CrossRefPubMedGoogle Scholar
  17. Li S, Jin H, Zhang Q (2016) The effect of exogenous spermidine concentration on polyamine metabolism and salt tolerance in Zoysiagrass (Zoysia japonica Steud) subjected to short-term salinity stress. Front Plant Sci 7:1221.  https://doi.org/10.3389/fpls.2016.01221 CrossRefPubMedGoogle Scholar
  18. Liang W, Ma X, Wan P, Liu L (2018) Plant salt-tolerance mechanism. Rev Biochem Biophys Res Commun 495:286–291.  https://doi.org/10.1016/j.bbrc.2017.11.043 CrossRefGoogle Scholar
  19. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls < em> a</em> and < em> b</em> of leaf extracts in different solvents Biochem Soc Trans 11:591–592  https://doi.org/10.1042/bst0110591 CrossRefGoogle Scholar
  20. Miyamoto N, Steudle E, Hirasawa T, Lafitte R (2001) Hydraulic conductivity of rice roots. J Exp Bot 52:1835–1846CrossRefPubMedGoogle Scholar
  21. Mostofa MG, Yoshida N, Fujita M (2014) Spermidine pretreatment enhances heat tolerance in rice seedlings through modulating antioxidative and glyoxalase systems. Plant Growth Regul 73:31–44.  https://doi.org/10.1007/s10725-013-9865-9 CrossRefGoogle Scholar
  22. Nayidu N, Bollina V, Kagale S (2013) Oilseed crop productivity under salt stress. In: Ahmad P, Azooz MM, Prasad MNV (eds) Ecophysiology and responses of plants under salt stress. Springer, New York, pp 249–265.  https://doi.org/10.1007/978-1-4614-4747-4_9 CrossRefGoogle Scholar
  23. Noctor G, Reichheld JP, Foyer CH (2017) ROS-related redox regulation and signaling in plants. Semin Cell Dev Biol.  https://doi.org/10.1016/j.semcdb.2017.07.013 CrossRefPubMedGoogle Scholar
  24. Pal M, Szalai G, Janda T (2015) Speculation: polyamines are important in abiotic stress signaling. Plant Sci 237:16–23.  https://doi.org/10.1016/j.plantsci.2015.05.003 CrossRefPubMedGoogle Scholar
  25. Patade VY, Bhargava S, Suprasanna P (2011) Salt and drought tolerance of sugarcane under iso-osmotic salt and water stress: growth, osmolytes accumulation, and antioxidant defense. J Plant Interact 6:275–282.  https://doi.org/10.1080/17429145.2011.557513 CrossRefGoogle Scholar
  26. Pérez-López U, Robredo A, Lacuesta M, Mena-Petite A, Muñoz-Rueda A (2009) The impact of salt stress on the water status of barley plants is partially mitigated by elevated CO2. Environ Exp Bot 66:463–470.  https://doi.org/10.1016/j.envexpbot.2009.03.007 CrossRefGoogle Scholar
  27. Saha J, Brauer EK, Sengupta A, Popescu SC, Gupta K, Gupta B (2015) Polyamines as redox homeostasis regulators during salt stress in plants Front Environ Sci.  https://doi.org/10.3389/fenvs.2015.00021 CrossRefGoogle Scholar
  28. Sequera-Mutiozabal M, Antoniou C, Tiburcio AF, Alcázar R, Fotopoulos V (2017) Polyamines: emerging hubs promoting drought and salt stress tolerance in plants current. Mol Biol Rep 3:28–36.  https://doi.org/10.1007/s40610-017-0052-z CrossRefGoogle Scholar
  29. Sgherri C, Perez-Lopez U, Micaelli F, Miranda-Apodaca J, Mena-Petite A, Munoz-Rueda A, Quartacci MF (2017) Elevated CO2 and salinity are responsible for phenolics-enrichment in two differently pigmented lettuces. Plant Physiol Biochem. 115:269–278.  https://doi.org/10.1016/j.plaphy.2017.04.006 CrossRefPubMedGoogle Scholar
  30. Tong H, Zhang ZX, Bin LI, Wang JW, Guo SR (2012) Effects of Iso-osmotic Ca(NO3)2 and NaCl stress on chloroplast ultra-structure and photosynthesis in cucumber leaves. China Veg 18:160–165Google Scholar
  31. Tong H, Guo SR, Zhang ZX, Sun J (2013) Effects of iso-osmotic Ca(NO3)2 and NaCl stress on ionic contents in different organs of cucumber seedlings. Acta Horticulturae 1004:181–188CrossRefGoogle Scholar
  32. Yi C et al (2015) High atmospheric carbon dioxide-dependent alleviation of salt stress is linked to RESPIRATORY BURST OXIDASE 1 (RBOH1)-dependent H2O2 production in tomato (Solanum lycopersicum). J Exp Bot 66:7391–7404.  https://doi.org/10.1093/jxb/erv435 CrossRefPubMedGoogle Scholar
  33. Zandalinas SI, Mittler R (2017) ROS-induced ROS release in plant and animal cells. Free Rad Biol Med.  https://doi.org/10.1016/j.freeradbiomed.2017.11.028 CrossRefPubMedGoogle Scholar
  34. Zhang Y et al (2013) Beneficial role of exogenous spermidine on nitrogen metabolism in tomato seedlings exposed to saline-alkaline stress. J Am Soc Hortic Sci 138:38–49Google Scholar
  35. Zhang Y, Zhang H, Zou Z-R, Liu Y, Hu X-H (2015) Deciphering the protective role of spermidine against saline–alkaline stress at physiological and proteomic levels in tomato. Phytochemistry 110:13–21.  https://doi.org/10.1016/j.phytochem.2014.12.021 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Horticulture/Collaborative Innovation Center of Quality and Profit Improvement for the Protected Vegetables of Shanxi ProvinceShanxi Agricultural UniversityTaiguPeople’s Republic of China
  2. 2.College of ForestryHenan University of Science and TechnologyLuoyangPeople’s Republic of China

Personalised recommendations