Effects of Potato Spindle Tuber Viroid Infection on Phytohormone and Antioxidant Responses in Symptomless Solanum laxum Plants
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To better understand the role of phytohormones and antioxidative responses in plant-viroid interaction, changes in endogenous phytohormone contents and activities of key antioxidant enzymes during latent infection of Solanum laxum by potato spindle tuber viroid (PSTVd) were examined. The results showed that endogenous jasmonic acid (JA) and castasterone (CS) content significantly increased, whereas abscisic acid content significantly decreased in symptomless leaves of systemically infected plants compared to that of mock-inoculated control plants. Regarding endogenous salicylic acid and indole-3-acetic acid content, there were no differences between infected and control plants. PSTVd infection also caused excessive production of hydrogen peroxide and enhanced the activity of ascorbate peroxidase (APX) and superoxide dismutase but not the activity of peroxidase and catalase (CAT). When expressed on a protein-weight base, only APX activity increased while CAT activity decreased almost by half in systemic leaves of infected plants. Furthermore, there was a fall in total glutathione content but also a rise in the content of oxidized forms of both ascorbate and glutathione, indicating that the regeneration of glutathione is insufficient. Results of this study strongly suggest the involvement of JA, CS and enzymatic and non-enzymatic antioxidants in the physiological response of S. laxum during latent infection by PSTVd.
KeywordsAntioxidants Brassinosteroids Jasmonic acid Latent infection Peroxidase PSTVd
This research was funded by the Ministry of Agriculture of the Republic of Croatia (National Survey of Quarantine Organisms Program), Croatian Centre for Agriculture, Food and Rural Affairs (PhD research fellowship to JM), and by the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. CR NPUI LO1204).
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Conflict of interest
The authors declare that they have no conflict of interest.
- Clarke SF, Guy PL. Jameson PE, Schmierer D, Burritt DJ (2000) Influence of white clover mosaic potexvirus infection on the endogenous levels of jasmonic acid and related compounds in Phaseolus vulgaris L. seedlings. J Plant Physiol 156:433–437. https://doi.org/10.1016/S0176-1617(00)80155-8 CrossRefGoogle Scholar
- Clouse SD, Feldmann KA (1999) Molecular genetics of brassinosteroid action. In: Sakurai A, Yokota T, Clouse SD (eds) Brassinosteroids: steroidal plant hormones. Springer, Tokyo, pp 163–190Google Scholar
- Mukherjee SP, Choudhuri MA (1983) Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170. https://doi.org/10.1111/j.1399-3054.1983.tb04162.x CrossRefGoogle Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232 Google Scholar
- Oklestkova J, Tarkowská D, Eyer L, Elbert T, Marek A, Smrzova Z, Novák O, Fránek M, Zhabinskii VN, Strnad M (2017) Immunoaffinity chromatography combined with tandem mass spectrometry: a new tool for the selective capture and analysis of brassinosteroid plant hormones. Talanta 170:432–440. https://doi.org/10.1016/j.talanta.2017.04.044 CrossRefGoogle Scholar
- Owens RA, Tech KB, Shao JY, Sano T, Baker CJ (2012) Global analysis of tomato gene expression during Potato spindle tuber viroid infection reveals a complex array of changes affecting hormone signalling. Mol Plant Microbe Interact 25:582–598. https://doi.org/10.1094/MPMI-09-11-0258 CrossRefGoogle Scholar
- Rizza S, Conesa A, Juarez J, Catara A, Navarro L. Duran-Vila N, Ancillo G (2012) Microarray analysis of Etrog citron (Citrus medica L.) reveals changes in chloroplast, cell wall, peroxidase and symporter activities in response to viroid infection. Mol Plant Pathol 13:852–886. https://doi.org/10.1111/j.1364-3703.2012.00794.x CrossRefGoogle Scholar