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Journal of Plant Growth Regulation

, Volume 38, Issue 1, pp 325–332 | Cite as

Effects of Potato Spindle Tuber Viroid Infection on Phytohormone and Antioxidant Responses in Symptomless Solanum laxum Plants

  • Jasna Milanović
  • Jana Oklestkova
  • Ondřej Novák
  • Snježana MihaljevićEmail author
Article
  • 154 Downloads

Abstract

To better understand the role of phytohormones and antioxidative responses in plant-viroid interaction, changes in endogenous phytohormone contents and activities of key antioxidant enzymes during latent infection of Solanum laxum by potato spindle tuber viroid (PSTVd) were examined. The results showed that endogenous jasmonic acid (JA) and castasterone (CS) content significantly increased, whereas abscisic acid content significantly decreased in symptomless leaves of systemically infected plants compared to that of mock-inoculated control plants. Regarding endogenous salicylic acid and indole-3-acetic acid content, there were no differences between infected and control plants. PSTVd infection also caused excessive production of hydrogen peroxide and enhanced the activity of ascorbate peroxidase (APX) and superoxide dismutase but not the activity of peroxidase and catalase (CAT). When expressed on a protein-weight base, only APX activity increased while CAT activity decreased almost by half in systemic leaves of infected plants. Furthermore, there was a fall in total glutathione content but also a rise in the content of oxidized forms of both ascorbate and glutathione, indicating that the regeneration of glutathione is insufficient. Results of this study strongly suggest the involvement of JA, CS and enzymatic and non-enzymatic antioxidants in the physiological response of S. laxum during latent infection by PSTVd.

Keywords

Antioxidants Brassinosteroids Jasmonic acid Latent infection Peroxidase PSTVd 

Notes

Acknowledgements

This research was funded by the Ministry of Agriculture of the Republic of Croatia (National Survey of Quarantine Organisms Program), Croatian Centre for Agriculture, Food and Rural Affairs (PhD research fellowship to JM), and by the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. CR NPUI LO1204).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute for Plant ProtectionCroatian Centre for Agriculture, Food and Rural AffairsZagrebCroatia
  2. 2.Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural ResearchPalacký University & Institute of Experimental Botany ASCROlomoucCzech Republic
  3. 3.Ruđer Bošković InstituteZagrebCroatia

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