Journal of Plant Growth Regulation

, Volume 38, Issue 1, pp 262–272 | Cite as

Semisynthetic Triterpenes Derived from Euphorbia officinarum as Plant Growth Promoters and Inducers of Disease Resistance

  • Amal Smaili
  • Lalla Aicha Rifai
  • Noureddine Mazoir
  • Tayeb Koussa
  • Lydia Faize
  • Nuria Alburquerque
  • Lorenzo Burgos
  • Kacem Makroum
  • Belfaiza Malika
  • Ahmed Benharref
  • Mohamed FaizeEmail author


This study evaluated the effect of application of the semisynthetic triterpenes 3β-acetoxy-norlup-20-one (F4) and 3-chloro-4α,14α-dimethyl-5α-cholest-8-ene (F6) triterpene derivatives from Euphorbia officinarum on the growth of tomato seedlings under normal conditions and when challenged with the pathogens Verticillium dahliae and Agrobacterium tumefaciens. Foliar spray of F4 and F6 significantly improved growth rate, fresh weight, dry weight, and leaf area. In addition, they enhanced several physiological parameters including photosynthetic pigments, proline content, and nitrate reductase activity. Moreover, they induced H2O2 accumulation and increased the activity of several antioxidant enzymes such as catalase, ascorbate peroxidase, and guaiacol peroxidase. They also enhanced disease resistance against V. dahliae and A. tumefaciens. These results suggest that the two semisynthetic triterpenes represent new plant growth regulators and inducers of plant disease resistance.


Antioxidant defenses Euphorbia officinarum Growth Triterpene derivatives Yield Disease resistance 



This research was supported by the University Chouaib Doukkali El Jadida (Morocco).

Compliance with Ethical Standards

Conflict of interest

No potential conflict of interest was reported by the authors.

Supplementary material

344_2018_9838_MOESM1_ESM.docx (1.9 mb)
Supplementary material 1 (DOCX 1974 KB)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Amal Smaili
    • 1
  • Lalla Aicha Rifai
    • 1
  • Noureddine Mazoir
    • 2
  • Tayeb Koussa
    • 1
  • Lydia Faize
    • 3
  • Nuria Alburquerque
    • 3
  • Lorenzo Burgos
    • 3
  • Kacem Makroum
    • 1
  • Belfaiza Malika
    • 1
  • Ahmed Benharref
    • 4
  • Mohamed Faize
    • 1
    Email author
  1. 1.Laboratory of Plant Biotechnology and Ecosystem Valorisation, Faculty of SciencesUniversity Chouaib DoukkaliEl JadidaMorocco
  2. 2.Department of Chemistry, Faculty of Sciences El JadidaUniversity Chouaib DoukkaliEl JadidaMorocco
  3. 3.Group of Fruit Tree Biotechnology, Department of Plant BreedingCEBAS-CSICMurciaSpain
  4. 4.Laboratory of Biomolecular Chemistry, Natural Substances and Reactivity, URAC 16, Faculty of Sciences SemlaliaCadi Ayyad UniversityMarrakechMorocco

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