Journal of Plant Growth Regulation

, Volume 38, Issue 1, pp 225–240 | Cite as

Gene Characterization and Expression Analysis Reveal the Importance of Auxin Signaling in Bud Dormancy Regulation in Tea Plant

  • Xinyuan Hao
  • Hu Tang
  • Bo Wang
  • Lu Wang
  • Hongli Cao
  • Yuchun Wang
  • Jianming Zeng
  • Shuang Fang
  • Jinfang Chu
  • Yajun YangEmail author
  • Xinchao WangEmail author


The tea plant is an economically important woody plant whose raw leaves are used for tea production. Winter bud dormancy is not only a useful biological strategy for tea plant survival but also a biological event that affects the economics of tea production. Based on our previous transcriptome analysis of axillary buds in different dormancy states, we reanalyzed a large number of differentially expressed auxin-related genes and determined the relative importance of the roles of auxin signaling in bud dormancy regulation in tea plant. Subsequently, we cloned the full-length cDNA sequence of several auxin-related genes in the AUX/LAX, PIN/PILS, AUX/IAA, GH3, and SAUR gene families, characterized these genes and performed a phylogenetic analysis, and conserved motif search using the sequences of their encoded proteins. Expression profile analyses, including tissue-specific expression and time-course expression during the active-dormant-active status transitions of overwinter buds, were carried out, combined with IAA content detection. Generally, the expression patterns of auxin-related genes were consistent with the IAA content changes in buds and their active-dormant status transition. In particular, we confirmed the crucial roles of the auxin transport gene CsLAX2 and the early auxin response genes CsGH3.6, CsGH3.9, CsGH3.10, CsIAA26, CsIAA33, CsSAUR50, and CsSAUR41 in bud dormancy regulation in tea plant. Our results validate the important role of auxin in tea plant dormancy regulation and provide useful information for further functional studies.


Auxin Bud dormancy Tea plant Expression profile IAA content 



This work was supported by the Zhejiang Provincial Natural Science Foundation (LY16C160001), the National Natural Science Foundation of China (31370690, 31600563), the Earmarked Fund for China Agriculture Research System (CARS-19), the Chinese Academy of Agricultural Sciences through an Innovation Project for Agricultural Sciences and Technology (CAAS-ASTIP-2017-TRICAAS), and CAS Key Technology Talent Program.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

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Authors and Affiliations

  1. 1.Tea Research InstituteChinese Academy of Agricultural SciencesHangzhouChina
  2. 2.National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources UtilizationMinistry of AgricultureHangzhouChina
  3. 3.College of HorticultureNorthwest A&F UniversityXianyangChina
  4. 4.National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina

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