Seed Priming with Iron Oxide Nanoparticles Triggers Iron Acquisition and Biofortification in Wheat (Triticum aestivum L.) Grains
Iron deficiency anaemia is a major challenge among consumers in developing countries. Given the deficiency of iron in the diet, there is an urgent need to devise a strategy for providing the required iron in the daily diet to counter the iron deficiency anaemia. We propose that iron biofortification of wheat (Triticum aestivum L.) through seed priming would be an innovative strategy to address this issue. This investigation attempts to find the interaction of iron oxide nanoparticles on germination, growth parameters and accumulation of grain iron in two contrasting wheat genotypes WL711 (low-iron genotype) and IITR26 (high-iron genotype). Wheat seeds were primed with different concentrations of iron oxide nanoparticles in the range of 25–600 ppm, resulting in differential accumulation of grain iron contents. We observed a pronounced increase in germination percentage and shoot length at 400 and 200 ppm treatment concentrations in IITR26 and WL711 genotypes, respectively. Intriguingly, the treatment concentration of 25 ppm demonstrated higher accumulation with a significant increase in grain iron contents to 45.7% in IITR26 and 26.8% in WL711 genotypes, respectively. Seed priming represents an innovative and user-friendly approach for wheat biofortification which triggers iron acquisition and accumulation in grains.
KeywordsIron oxide nanoparticles Seed priming Iron accumulation Biofortification
We gratefully acknowledge the financial support provided by the Department of Molecular Biology & Genetic Engineering and Govind Ballabh Pant University of Agriculture and Technology, Pantnagar to conduct this study. We thank Dr. Joy Kumar Roy, Scientist-E, National Agri-Food Biotechnology Institute (NABI), India for supplying the wheat seed samples.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Buettner GR, Jurkiewicz BA (1996) Chemistry and biochemistry of ascorbic acid. In: Cadenas E, Packer L (eds) Handbook of antioxidants. Marcel Dekker, New York, pp 91–115Google Scholar
- Schopfer P, Plachy C, Frahry G (2001) Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol 125:1591–1602CrossRefPubMedPubMedCentralGoogle Scholar
- Singh SP, Jeet R, Kumar J, Shukla V, Srivastava R, Mantri SS, Tuli R (2014) Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. PLoS ONE. https://doi.org/10.1371/journal.pone.0111718 CrossRefPubMedPubMedCentralGoogle Scholar