Journal of Plant Growth Regulation

, Volume 37, Issue 3, pp 859–866 | Cite as

Effect of Azospirillum brasilense Sp245 Lipopolysaccharides on Wheat Plant Development

  • Estefanía Chávez-Herrera
  • Alma Alejandra Hernández-Esquivel
  • Elda Castro-Mercado
  • Ernesto García-PinedaEmail author


Lipopolysaccharides (LPS) are integral and essential constituents of the outer membranes of Gram-negative bacteria that have been extensively investigated in relation to the activation of plant defense responses. In the present study, we evaluated the effect of exogenously applied Azospirillum brasilense LPS on wheat plant development, including plant aging, spike formation and size, as well as grain yield and grain chemical composition. Experiments were performed in plants cultivated in pots under greenhouse conditions. Plants were sprayed with two LPS concentrations (2 and 5 µg/mL) once a week over 3 months. LPS administration increased leaf length, especially the second leaf. Although spike formation was accelerated, spike length was reduced compared to untreated controls, and both responses were dependent on LPS concentration. Plant aging was also accelerated, and the dry weight of plants increased when treated with 5 µg/mL LPS. Moreover, the impact of exogenous LPS treatment on the protein, starch, and lipid content in grains was relatively negligible. Our results showed that A. brasilense LPS affected some aspects of wheat development such as plant aging and spike formation, but not grain chemical composition or grain yield.


Lipoplysaccharides Wheat Azospirillum brasilense Spike Rhizobacteria 



This study was supported by the Coordinación de la Investigación Científica, Universidad Michoacana de San Nicolás de Hidalgo, México.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Instituto de Investigaciones Químico BiológicasUniversidad Michoacana de San Nicolás de HidalgoMoreliaMexico

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