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Journal of Plant Growth Regulation

, Volume 37, Issue 4, pp 1385–1395 | Cite as

Fluridone Induces Leaf Bleaching by Inhibiting Pigment Biosynthesis via Downregulated Transcription Levels of Pigment Biosynthetic Genes in Rice (Oryza Sativa L.)

  • Zhengshan Zou
  • Xiao Zou
  • Songtao Zhao
  • Chan Xia
  • Ke Qian
  • Pei Wang
  • Changxi YinEmail author
Article

Abstract

Fluridone (FLU) is widely used as a herbicide and interferes with photosynthesis via induction of leaf bleaching. However, the mechanism of FLU-induced leaf bleaching remains elusive. In this study, the effects of FLU on the metabolism of leaf pigments, including chlorophyll and carotenoids, were investigated. Our results demonstrate that FLU induced rice leaf bleaching in a dose-dependent manner. Treatment with 5 µM FLU strongly induced leaf bleaching by decreasing pigment content, with carotenoids and chlorophyll decreased by 98 and 95%, respectively, in the second leaves of rice seedlings. Our results indicate that the transcription levels of leaf pigment biosynthetic and catabolic genes were significantly downregulated by 5 µM FLU treatment. These results suggest that FLU induces leaf bleaching by decreasing leaf pigment content via downregulation of the transcription levels of leaf pigment biosynthetic genes, and that the downregulation of transcription levels of leaf pigment catabolic genes is a result of feedback inhibition mediated by FLU-decreased leaf pigment content. In addition, to test whether FLU-induced leaf bleaching is due to FLU-induced abscisic acid (ABA) deficiency, the effect of FLU treatment on endogenous ABA content and the recovery effect of ABA on FLU-induced leaf bleaching were investigated. Application of FLU significantly decreased endogenous ABA content, but FLU-induced leaf bleaching was not rescued by ABA application, thus FLU-induced leaf bleaching is not due to FLU-induced ABA deficiency.

Keywords

Abscisic acid Carotenoid Chlorophyll Fluridone Leaf bleaching Rice 

Notes

Acknowledgements

This work was supported by The National Key Research and Development Program of China (No. 2016YFD0300102), the Natural Science Foundation of Hubei Province of China (No. 2016CFB436), China Postdoctoral Science Foundation (Nos. 2015M580652, 2016T90705), and the Fundamental Research Funds for the Central Universities (2662015PY158).

Author Contributions

ZZS and ZX performed most of the experiments. ZST, XC, QK, and WP were involved in the experiments. ZZS, ZX, and YCX analysed the data. YCX designed the experiments and wrote the manuscript. All authors reviewed the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. 1.College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Jiangsu Academy of Agricultural Sciences Seed Industry Research Institute Co., LtdNanjingPeople’s Republic of China

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