Journal of Plant Growth Regulation

, Volume 35, Issue 4, pp 1049–1063 | Cite as

Differential Expression of Methyl Jasmonate-Responsive Genes Correlates with Laticifer Vessel Proliferation in Phloem Tissue of Rubber Tree (Hevea brasiliensis)

  • Teerawat Laosombut
  • Poochita Arreewichit
  • Kanlaya Nirapathpongporn
  • Paweena Traiperm
  • Panida Kongsawadworakul
  • Unchera Viboonjun
  • Jarunya Narangajavana


Rubber tree (Hevea brasiliensis) is a pivotal source for natural rubber production. Polyisoprene is synthesized in laticifer vessels, which are developed from vascular cambium in the phloem area of the inner bark tissue. The transcription factors and phytohormones were reported to be involved in network regulation of plant vascular tissue differentiation. Methyl jasmonate (MeJA) was reported to act as a stimulator for laticifer vessel formation in rubber tree, but the regulatory mechanism remains largely unknown. In this study, the correlation of laticifer vessel proliferation in phloem tissue upon MeJA treatment and the MeJA-responsive gene expression was investigated. Histochemical study of the laticifer revealed the circle and diffused structure around the secondary phloem of the stem. The number of laticifer vessels was increased from the top shoot to the lower stem part. The higher average number of laticifer vessels in stems of high latex-producing clones than in low latex/high wood yield clones suggested the correlation of laticifer vessel number and latex yield potential. This study demonstrated the temporal differential expression of MeJA-responsive genes upon MeJA treatment and adjusted back to a normal level after 3 months in two high latex-producing clones. The increasing number of laticifer vessels in MeJA-treated plants confirmed the consequent effect of MeJA treatment, and the possible roles of these genes in relation with laticifer vessel proliferation are discussed. A better understanding of gene function in laticifer development would be beneficial in rubber tree improvement and exploitation.


Laticifer vessel Methyl jasmonate Rubber tree Vascular tissue differentiation 



This work was supported by Mahidol University. Teerawat Laosombut was supported by Institutional Strengthening Program, Faculty of Science, Mahidol University, Thailand. The authors thank Ms. Wipa Arsingsamanan and Ms. Palita Sittivach for technical support and assistance in histochemical study of the laticifer structure.

Authors’ Contribution

Jarunya Narangajavana designed the experiments. Teerawat Laosombut performed the experiments and drafted the manuscript. Poochita Arreewichit performed some parts of the experiments. Kanlaya Nirapathpongporn provided plant materials. Paweena Traiperm, Panida Kongsawadworakul, Unchera Viboonjun, and Jarunya Narangajavana provided technical support and discussed the results. Jarunya Narangajavana provided the funding for this work and is the corresponding author.

Supplementary material

344_2016_9603_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1122 kb)


  1. Baima S, Nobili F, Sessa G, Lucchetti S, Ruberti I, Morelli G (1995) The expression of the Athb-8 homeobox gene is restricted to provascular cells in Arabidopsis thaliana. Development 121:4171–4182PubMedGoogle Scholar
  2. Baima S, Possenti M, Matteucci A, Wisman E, Altamura MM, Ruberti I et al (2001) The arabidopsis ATHB-8 HD-zip protein acts as a differentiation-promoting transcription factor of the vascular meristems. Plant Physiol 126(3):64443–64455Google Scholar
  3. Baucher M, Jaziri ME, Vandeputte O (2007) From primary to secondary growth: origin and development of the vascular system. J Exp Bot 58(13):3485–3501CrossRefPubMedGoogle Scholar
  4. Carlsbecker A, Helariutta Y (2005) Phloem and xylem specification: pieces of the puzzle emerge. Plant Biol 8:512–517Google Scholar
  5. Chini A, Fonseca S, Fernandez G, Adie B, Chico JM, Lorenzo O et al (2007) The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448:666–671CrossRefPubMedGoogle Scholar
  6. Chow KS, Mat-Isa MN, Bahari A, Ghazali AK, Alias H, Zainuddin ZM et al (2012) Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. J Exp Bot 63(5):1863–1871CrossRefPubMedGoogle Scholar
  7. de Fäy E, Jacob JL (1989) Anatomical organization of the laticiferous system in Hevea bark. In: d’Auzac J, Jacob JL, Chrestin H (eds) Physiology of the rubber tree latex. CRC Press, Boca Raton, pp 3–15Google Scholar
  8. Duan C, Rio M, Leclercq J, Bonnot F, Oliver G, Montoro P (2010) Gene expression pattern in response to wounding, methyl-jasmonate and ethylene in the bark of Hevea brasiliensis. Tree Physiol 30:1349–1359CrossRefPubMedGoogle Scholar
  9. Farmer EE, Ryan CA (1990) Interplant communication: airborne methyl-jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Natl Acad Sci USA 87:7713–7716CrossRefPubMedPubMedCentralGoogle Scholar
  10. Hao BZ, Wu JL (2000) Laticifer differentiation in Hevea brasiliensis: induction by exogenous jasmonic acid and linolenic acid. Ann Bot 85:37–43CrossRefGoogle Scholar
  11. Hussey SG, Mizrachi E, Creux NM, Myburg AA (2013) Navigating the transcriptional roadmap regulating plant secondary cell wall deposition. Front Plant Sci 4:325CrossRefPubMedPubMedCentralGoogle Scholar
  12. Kachroo P (2013) The plant vascular system: evolution, development and functions. J Integr Plant Biol 55(4):294–388CrossRefPubMedGoogle Scholar
  13. Karpinska B, Karlsson M, Srivastava M, Stenberg A, Schrader J, Sterky F et al (2004) MYB transcription factors are differentially expressed and regulated during secondary vascular tissue development in hybrid aspen. Plant Mol Biol 56(2):255–270CrossRefPubMedGoogle Scholar
  14. Katsir L, Chung HS, Abraham JKK, Gregg AH (2008a) Jasmonate signaling: a conserved mechanism of hormone sensing. Plant Biol 11:428–435Google Scholar
  15. Katsir L, Schilmiller AL, Staswick PE, Sheng HE, Howe GA (2008b) COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. Proc Natl Acad Sci USA 105(19):7100–7105CrossRefPubMedPubMedCentralGoogle Scholar
  16. Ko JH, Jeon HW, Kim WC, Kim JY, Han KH (2004) The MYB46/MYB83-mediated transcriptional regulatory programme is a gatekeeper of secondary wall biosynthesis. Ann Bot 114:1099–1107CrossRefGoogle Scholar
  17. Lehesranta SJ, Lichtenberger R, Helariutta Y (2010) Cell-to-cell communication in vascular morphogenesis. Plant Biol 13:59–65Google Scholar
  18. Lertpanyasampatha M, Viboonjun U, Kongsawadworakul P, Chrestin H, Narangajavana J (2014) Differential expression of microRNAs and their targets reveals a possible dual role in physiological bark disorder in rubber tree. J Plant Physiol 171:1117–1126CrossRefPubMedGoogle Scholar
  19. Lescot M, Dhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y et al (2002) Plant-CARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325–327CrossRefPubMedPubMedCentralGoogle Scholar
  20. Li H, Qin Y, Xiao X, Tang C (2011) Screening of valid reference genes for real-time RT-PCR data normalization in Hevea brasiliensis and expression validation of a sucrose transporter gene HbSUT3. Plant Sci 181:132–139CrossRefPubMedGoogle Scholar
  21. Lucas WJ, Groover A, Lichtenberger R, Furuta K, Yadav SR, Helariutta Y et al (2005) The arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol 138(2):1083–1096CrossRefGoogle Scholar
  22. Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR et al (2010) Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature 468:400–405CrossRefPubMedPubMedCentralGoogle Scholar
  23. Tabata R, Ikezaki M, Fujibe T, Aida M, Tian C, Ueno Y et al (2010) Arabidopsis AUXIN RESPONSE FACTOR6 and 8 regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes. Plant Cell Physiol 51(1):164–175CrossRefPubMedGoogle Scholar
  24. Tan D, Sun X, Zhang J (2011) Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis. Plant Cell Rep 30(6):1117–1124CrossRefPubMedGoogle Scholar
  25. Tan D, Sun X, Zhang J (2014) Age dependent and jasmonic acid induced laticifer cell differentiation in anther callus cultures of rubber tree. Planta 240:337–344CrossRefPubMedGoogle Scholar
  26. Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G et al (2007) JAZ repressor proteins are targets of the SCF (COI1) complex during jasmonate signalling. Nature 448:661–665CrossRefPubMedGoogle Scholar
  27. Tiwari SB, Hagen G, Guilfoyle T (2003) The roles of auxin response factor domains in auxin-responsive transcription. Plant Cell 5(2):533–543CrossRefGoogle Scholar
  28. Zhao Y, Sun J, Xu P, Zhang R, Li L (2014) Intron-mediated alternative splicing of WOOD-ASSOCIATED NAC TRANSCRIPTION FACTOR1B regulates cell wall thickening during fiber development in Populus species. Plant Physiol 164:765–776CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Teerawat Laosombut
    • 1
  • Poochita Arreewichit
    • 1
  • Kanlaya Nirapathpongporn
    • 2
  • Paweena Traiperm
    • 3
  • Panida Kongsawadworakul
    • 3
  • Unchera Viboonjun
    • 3
  • Jarunya Narangajavana
    • 1
  1. 1.Department of Biotechnology, Faculty of ScienceMahidol UniversityRajtheweeThailand
  2. 2.Rubber Research Institute of Thailand (RRIT)BangkokThailand
  3. 3.Department of Plant Science, Faculty of ScienceMahidol UniversityBangkokThailand

Personalised recommendations