Journal of Plant Growth Regulation

, Volume 31, Issue 4, pp 579–587

PEG and ABA Trigger the Burst of Reactive Oxygen Species to Increase Tanshinone Production in Salvia miltiorrhiza Hairy Roots

  • Dongfeng Yang
  • Dongfeng Sheng
  • Qimei Duan
  • Xiao Liang
  • Zongsuo Liang
  • Yan Liu
Article

DOI: 10.1007/s00344-012-9268-6

Cite this article as:
Yang, D., Sheng, D., Duan, Q. et al. J Plant Growth Regul (2012) 31: 579. doi:10.1007/s00344-012-9268-6

Abstract

Salvia miltiorrhiza is one of the most popular traditional Chinese medicinal plants because of its excellent performance in treating coronary heart disease. Tanshinones, a group of active compounds in S. miltiorrhiza, are derived from two biosynthetic pathways: the mevalonate (MVA) pathway in the cytosol and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway in the plastids. Water stress is well known to stimulate the accumulation of secondary metabolites in plants. Reactive oxygen species (ROS) serve as important secondary messengers in water stress-induced signal transduction pathways. In this study, the effects of polyethylene glycol (PEG) and abscisic acid (ABA) on tanshinone production in S. miltiorrhiza hairy roots were investigated and the roles of ROS in PEG- and ABA-induced tanshinone production were further elucidated. The results showed that contents and yields of four tanshinones in S. miltiorrhiza hairy roots were significantly enhanced by 2 % PEG and 200 μM ABA. Simultaneously, the mRNA levels and activities of two key enzymes (3-hydroxy-3-methylglutaryl coenzyme A reductase and 1-deoxy-D-xylulose 5-phosphate synthase) involved in tanshinone biosynthesis were upregulated. Both PEG and ABA were able to trigger the burst of H2O2 and O2. The PEG- and ABA-induced increases of tanshinone production, gene expression, and enzyme activity were all dramatically suppressed by two ROS scavengers, catalase and superoxide dismutase. In addition, ROS treatments resulted in a significant increase in tanshinone production. These results demonstrated that the MVA and MEP pathways were activated by PEG and ABA to stimulate tanshinone biosynthesis, and the increase of tanshinone production was probably via ROS signaling.

Keywords

Salvia miltiorrhiza Tanshinone Water stress Abscisic acid Reactive oxygen species 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Dongfeng Yang
    • 1
  • Dongfeng Sheng
    • 2
  • Qimei Duan
    • 1
  • Xiao Liang
    • 1
  • Zongsuo Liang
    • 1
    • 3
  • Yan Liu
    • 4
  1. 1.College of Life ScienceNorthwest A&F UniversityYanglingChina
  2. 2.Department of Life ScienceZhoukou Normal UniversityZhoukouChina
  3. 3.College of Life ScienceZhejiang Sci-Tech UniversityHangzhouChina
  4. 4.Tianjin Tasly Modern TCM Resources Co., LtdTianjinChina

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