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Journal of Plant Growth Regulation

, Volume 31, Issue 3, pp 332–341 | Cite as

Effects of Salinity on Metabolic Profiles, Gene Expressions, and Antioxidant Enzymes in Halophyte Suaeda salsa

  • Huifeng Wu
  • Xiaoli Liu
  • Liping You
  • Linbao Zhang
  • Di Zhou
  • Jianghua Feng
  • Jianmin Zhao
  • Junbao Yu
Article

Abstract

Halophyte Suaeda salsa is native to the saline soil in the Yellow River Delta. Soil salinity can reduce plant productivity and therefore is the most important factor for the degradation of wetlands in the Yellow River Delta. In this work we characterized the salinity-induced effects in S. salsa in terms of metabolic profiling, antioxidant enzyme activities, and gene expression quantification. Our results showed that salinity inhibited plant growth of S. salsa and upregulated gene expression levels of myo-inositol-1-phosphate synthase (INPS), choline monooxygenase (CMO), betaine aldehyde dehydrogenase (BADH), and catalase (CAT), and elevated the activities of superoxide dismutase (SOD), peroxidase (POD), CAT, and glutathione peroxidase (GPx). The significant metabolic responses included the depleted amino acids malate, fumarate, choline, phosphocholine, and elevated betaine and allantoin in the aboveground part of S. salsa seedlings as well as depleted glucose and fructose and elevated proline, citrate, and sucrose in root tissues. Based on these significant biological markers, salinity treatments induced clear osmotic stress (for example, INPS, CMO, BADH, betaine, proline) and oxidative stress (for example, SOD, POD, CAT, GPx activities), disturbed protein biosynthesis/degradation (amino acids and total protein) and energy metabolism (for example, glucose, sucrose, citrate) in S. salsa.

Keywords

Suaeda salsa Salinity Metabolomics Antioxidant enzyme activity Gene expression 

Notes

Acknowledgments

We thank Dr. Mark Viant (School of Bioscience, The University of Birmingham) for use of the software ProMetab. This research was supported by the Project of National Science & Technology Pillar Program in “12th Five Year” Period (2011BAC02B01), The 100 Talents Program of the Chinese Academy of Sciences, Innovation Programs of the Chinese Academy of Sciences (KZCX2-YW-223 and KZCX2-YW-225), and Technology Development Program Projects of Shandong Province (2008GG20005006 and 2008GG3NS0700), and in part by the CAS/SAFEA International Partnership Program for Creative Research Teams.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Huifeng Wu
    • 1
    • 2
  • Xiaoli Liu
    • 1
    • 2
    • 3
  • Liping You
    • 1
    • 2
    • 3
  • Linbao Zhang
    • 1
    • 2
    • 3
  • Di Zhou
    • 1
    • 2
    • 3
  • Jianghua Feng
    • 4
  • Jianmin Zhao
    • 1
    • 2
  • Junbao Yu
    • 1
    • 2
  1. 1.Key Laboratory of Coastal Zone Environment ProcessesChinese Academy of SciencesYantaiPeople’s Republic of China
  2. 2.Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiPeople’s Republic of China
  3. 3.The Graduate School of Chinese Academy of SciencesBeijingPeople’s Republic of China
  4. 4.Department of Electronic Science, Fujian Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid SurfacesXiamen UniversityXiamenPeople’s Republic of China

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