Journal of Plant Growth Regulation

, Volume 30, Issue 2, pp 229–234 | Cite as

Application of Digital Image Analysis System for Fine Evaluation of Varietal Differences and the Role of Ethylene in Visible Petal Senescence of Morning Glory

  • Yoshihito Shinozaki
  • Takanari Tanabata
  • Isao Ogiwara
  • Tetsuya Yamada
  • Motoki Kanekatsu


We detected differences in both onset and progression of visible petal senescence among morning glory cultivars by application of a digital image analysis system. The system is based on semiautomated time-lapse measurement of corolla areas. The system could also be applied to evaluate the effects of ethylene and its inhibitor on visible petal senescence. Both onset and progression of visible petal senescence were accelerated by ethylene treatment in all six cultivars tested. Treatment with aminooxyacetic acid (AOA), an ethylene biosynthesis inhibitor, prolonged time to onset of visible petal senescence in three of the six tested cultivars. In contrast, AOA treatment had no effect on duration of visible petal senescence in any tested cultivars. These data suggested differences among morning glory cultivars in the role of endogenous ethylene in controlling onset of visible petal senescence. In addition, we propose a new application of image analysis to fine quantification of time-lapse changes in the shape of plant organs.


Flower longevity Ipomoea nil Morphological character Phenotypic variation Quantitative determination Shape analysis 



The authors thank Dr. Eiji Nitasaka and the National BioResource Project, Japan, for providing seeds of Q61, Q62, Q65, Q438, and Q1065, and Dr. Kenichi Shibuya for his technical assistance with ethylene measurement. This work was supported by a Japan Society for the Promotion of Science Grant-in-Aid for Young Scientists (Start-up) (no. 18880009 to T.Y.) and by a Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Young Scientists (B) (no. 19780019 to T.Y.).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yoshihito Shinozaki
    • 1
  • Takanari Tanabata
    • 2
  • Isao Ogiwara
    • 1
  • Tetsuya Yamada
    • 1
  • Motoki Kanekatsu
    • 1
  1. 1.Department of Plant Production, United Graduate School of Agricultural ScienceTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.National Institute of Agrobiological SciencesTsukubaJapan

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