Journal of Plant Growth Regulation

, Volume 29, Issue 3, pp 307–315 | Cite as

Paclobutrazol Arrests Vegetative Growth and Unveils Unexpressed Yield Potential of Jatropha curcas

  • Arup Ghosh
  • Jitendra Chikara
  • D. R. Chaudhary
  • Aruna R. Prakash
  • G. Boricha
  • A. Zala


Although the process for making EN 14214 grade Jatropha methyl ester (biodiesel) capable of running unmodified diesel engines in neat form has been demonstrated, getting higher seed yield from Jatropha shrubs in wastelands is critical to the success of Jatropha biodiesel. But, low productivity is inherent to many Jatropha curcas germplasms and raising large-scale plantations using such untested planting material can lead to wasteful expenditures. Unreliable and poor flowering and fruiting are important factors responsible for low productivity in the species. Although much is known about growth retardants applied to field and horticultural crops, their role in improving the seed productivity of Jatropha has never been explored. Here we report for the first time that paclobutrazol could be an extremely useful chemical, whose dose and time of application, if optimized, can significantly reduce unwanted vegetative growth, with concomitant improvement in yield and seed oil content of Jatropha. In the year following application of paclobutrazol, an unexpected increase in seed yield, as high as 1127% relative to controls, was obtained from one such unproductive Jatropha germplasm. We hypothesize that low seed production in this species may be a result of excess vegetative growth caused by an unfavorable endogenous hormonal configuration which competes with growth and development of flower, fruit, or seed. This undesired physiological state can be reversed by paclobutrazol application to achieve maximum oil yield from this energy shrub that holds great promise in the future.


Jatropha curcas Biodiesel Paclobutrazol Seed yield Oil content Nutrient uptake 



We thank Dr. P. K. Ghosh for critical discussions and reading of the manuscript; Dr. J. S. Patolia and Mr. D. R. Parmar for support; Mr. M. N. Verma from Syngenta Ltd. for providing paclobutrazol for experimentation; Dr. Ananta Sarkar for statistical assistance; and Discipline of Analytical Sciences, CSMCRI for technical help with chemical analysis. We also gratefully acknowledge the financial support from DaimlerChrysler AG, Stuttgart and DEG, Germany, the Government of Gujarat, and the Council of Scientific and Industrial Research (CSIR), New Delhi, India.


  1. AOAC (Association of Official Analytical Chemists) (1970) Official methods of analysis. AOAC, Washington, DCGoogle Scholar
  2. Aron Y, Monselise SP, Goren R, Costo J (1985) Chemical control of vegetative growth in citrus trees by paclobutrazol. HortScience 20:96–98Google Scholar
  3. Asin L, Alegre S, Montserrat R (2007) Effect of paclobutrazol, prohexadione-Ca, deficit irrigation, summer pruning and root pruning on shoot growth, yield, and return bloom, in a ‘Blanquilla’ pear orchard. Sci Hortic 113:142–148CrossRefGoogle Scholar
  4. Belakbir A (1998) Yield and fruit quality of pepper (Capsicum annum L.) in response to bioregulators. HortScience 33:85–87Google Scholar
  5. Berova M, Zlatev Z (2000) Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentum Mill.). Plant Growth Regul 30:117–123CrossRefGoogle Scholar
  6. Blaikie SJ, Kulkarni VJ, Muller WJ (2004) Effects of morphactin and paclobutrazol flowering treatments on shoot and root phenology in mango cv. Kensington Pride. Sci Hortic 101:51–68CrossRefGoogle Scholar
  7. Blanco A (1990) Effects of paclobutrazol and of ethephon on cropping and vegetative growth of “Crimson Gold” nectarine trees. Sci Hortic 42:65–73CrossRefGoogle Scholar
  8. Burch PL, Wells RH, Kline WL III (1996) Red maple and silver maple growth evaluated 10 years after application of paclobutrazol tree growth regulator. J Arboric 22:61–66Google Scholar
  9. Day PR (1965) Particle fraction and particle size analysis. In: Black CA et al (eds) Methods of soil analysis part 1. ASA, Madison, pp 545–567Google Scholar
  10. Erez A (1986) Growth control with paclobutrazol of peaches grown in meadow orchard system. Acta Hortic 160:217–224Google Scholar
  11. Fairless D (2007) Biofuel: the little shrub that could—maybe. Nature 449:652–655CrossRefPubMedGoogle Scholar
  12. Gent MPN (1997) Persistence of triazole growth retardants on stem elongation of Rhododendron and Kalmia. J Plant Growth Regul 16:197–203CrossRefGoogle Scholar
  13. Ghosh A, Chaudhary DR, Reddy MP, Rao SN, Chikara J, Pandya JB, Patolia JS, Gandhi MR, Adimurthy S, Vaghela N, Mishra S, Rathod MR, Prakash AR, Shethia BD, Upadhyay SC, Balakrishna V, Prakash CR, Ghosh PK (2007) Prospects for jatropha methyl ester (biodiesel) in India. Int J Environ Stud 64:659–674CrossRefGoogle Scholar
  14. Graebe JE (1987) Gibberellin biosynthesis and control. Annu Rev Plant Physiol 38:419–465CrossRefGoogle Scholar
  15. Grochowska MJ, Hodun M (1997) The dwarfing effect of a single application of growth inhibitor to the root-stem connection—“the collar tissue”—of five species of fruit trees. J Hortic Sci 72:83–91Google Scholar
  16. Hanway JJ, Heidel H (1952) Soil analysis methods as used in Iowa State College soil testing laboratory. Iowa Agric 57:1–31Google Scholar
  17. Hasan O, Reid JB (1995) Reduction of generation time in Eucalyptus globules. Plant Growth Regul 17:53–60Google Scholar
  18. Haughan PA, Burden RS, Lenton JR, Goad LJ (1989) Inhibition of celery cell growth and sterol biosynthesis by the enantiomers of paclobutrazol. Phytochemistry 28:781–787CrossRefGoogle Scholar
  19. Hedden P, Graebe JE (1985) Inhibition of gibberellin biosynthesis by paclobutrazol in cell-free homogenates of Cucurbita maxima endosperm and Malus pumila embryos. J Plant Growth Regul 4:111–122CrossRefGoogle Scholar
  20. Jackson ML (1973) Soil chemical analysis. Prentice Hall, New DelhiGoogle Scholar
  21. Jacyna T, Sparrow SM, Dodds KG (1989) Paclobutrazol in managing mature cropping apricot trees. Acta Hortic 240:139–142Google Scholar
  22. Jonschaap REE, Corre WJ, Bindraban PS, Brandenburg WA (2007) Claims and Facts on Jatropha curcas L., Report 158. Plant Research International B.V., WageningenGoogle Scholar
  23. Khalil IA (1995) Chlorophyll and carotenoid contents in cereals as affected by growth retardants of triazole series. Cereal Res Commun 23:183–189Google Scholar
  24. Khurshid T, McNeil DL, Trought MCT, Hill GD (1997) The response of young ‘Braeburn’ and ‘Oregon Spur Delicious’ apple trees growing under an ultra-high density planting system to soil-applied paclobutrazol: I. Effect on reproductive and vegetative growth. Sci Hortic 72:11–24CrossRefGoogle Scholar
  25. Kochhar S, Kochhar VK, Singh SP, Katiyar RS, Pushpangadan P (2005) Differential rooting and sprouting behaviour of two Jatropha species and associated physiological and biochemical changes. Curr Sci 89:936–939Google Scholar
  26. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592Google Scholar
  27. Lindsay WL, Norvell WA (1978) Development of DTPA soil test for Zn, Fe, Mn, and Cu. Soil Sci Soc Am J 42:421–428CrossRefGoogle Scholar
  28. Ma FW, Wang JC, Rong W (1990) Effect of plant growth regulators on in vitro propagation of apple cultivar Fuji. J Fruit Sci 7:201–206Google Scholar
  29. Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular 939. U.S. GPO, Washington, DCGoogle Scholar
  30. Richards LA (1954) Diagnosis and improvement of saline alkali soils, handbook no. 60. USDA, Washington, DCGoogle Scholar
  31. SalazarGarcia S, VazquezValdivia V (1997) Physiological persistence of paclobutrazol on the ‘Tommy Atkins’ mango (Mangifera indica L.) under rainfed conditions. J Hortic Sci 72:339–345Google Scholar
  32. Sebastian B, Alberto G, Emilio AC, Jose AF, Juan AF (2002) Growth, development and color response of potted Dianthus caryophyllus cv. Mondriaan to paclobutrazol treatment. Sci Hortic 94:371–377CrossRefGoogle Scholar
  33. Senoo S, Isoda A (2003) Effects of paclobutrazol on dry matter distribution and yield in peanut. Plant Prod Sci 6:90–94CrossRefGoogle Scholar
  34. Subbiah BV, Asija GL (1956) A rapid procedure for the determination of available nitrogen in soils. Curr Sci 25:259–260Google Scholar
  35. Walkley AJ, Black IA (1934) Estimation of soil organic carbon by chromic acid titration method. Soil Sci 37:29–38CrossRefGoogle Scholar
  36. Williams MW, Curry EA, Greene GM (1986) Chemical control of vegetative growth of pome and stone fruit trees with GA biosynthesis inhibitors. Acta Hortic 179:453–458Google Scholar
  37. Wood BW (1988) Paclobutrazol, uniconazole, and flurprimidol influence shoot growth and nut yield of young pecan trees. HortScience 23:1026–1028Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Discipline of Wasteland ResearchCentral Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research, New Delhi)BhavnagarIndia

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